Experiment: Vancomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3514 and Pf6N2E2_3515 are separated by 30 nucleotides Pf6N2E2_3515 and Pf6N2E2_3516 are separated by 13 nucleotides Pf6N2E2_3516 and Pf6N2E2_3517 are separated by 147 nucleotides
Pf6N2E2_3514 - Ribosomal protein L11 methyltransferase (EC 2.1.1.-), at 4,098,951 to 4,099,829
_3514
Pf6N2E2_3515 - Probable transmembrane protein, at 4,099,860 to 4,100,942
_3515
Pf6N2E2_3516 - Probable transmembrane protein, at 4,100,956 to 4,101,090
_3516
Pf6N2E2_3517 - tRNA dihydrouridine synthase B (EC 1.-.-.-), at 4,101,238 to 4,102,245
_3517
Position (kb)
4099
4100
4101 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4098.889 kb on + strand at 4098.889 kb on + strand at 4098.926 kb on + strand at 4098.926 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.967 kb on + strand at 4098.968 kb on - strand at 4098.968 kb on - strand at 4098.968 kb on - strand at 4099.002 kb on + strand at 4099.002 kb on + strand at 4099.002 kb on + strand at 4099.003 kb on - strand at 4099.024 kb on + strand at 4099.024 kb on + strand at 4099.025 kb on - strand at 4099.587 kb on + strand, within Pf6N2E2_3514 at 4099.587 kb on + strand, within Pf6N2E2_3514 at 4099.588 kb on - strand, within Pf6N2E2_3514 at 4099.755 kb on + strand at 4099.756 kb on - strand at 4099.827 kb on + strand at 4099.842 kb on + strand at 4100.256 kb on + strand, within Pf6N2E2_3515 at 4100.256 kb on + strand, within Pf6N2E2_3515 at 4100.603 kb on - strand, within Pf6N2E2_3515 at 4100.603 kb on - strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.723 kb on + strand, within Pf6N2E2_3515 at 4100.723 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.923 kb on + strand at 4101.022 kb on + strand, within Pf6N2E2_3516 at 4101.022 kb on + strand, within Pf6N2E2_3516 at 4101.023 kb on - strand, within Pf6N2E2_3516 at 4101.129 kb on + strand at 4101.129 kb on + strand at 4101.130 kb on - strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.262 kb on + strand at 4101.262 kb on + strand at 4101.262 kb on + strand at 4101.262 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.775 kb on + strand, within Pf6N2E2_3517 at 4101.775 kb on + strand, within Pf6N2E2_3517
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml remove 4,098,889 + -0.0 4,098,889 + -1.5 4,098,926 + +1.1 4,098,926 + +0.3 4,098,967 + +0.1 4,098,967 + -2.3 4,098,967 + +0.1 4,098,967 + -0.7 4,098,967 + -0.8 4,098,967 + -2.3 4,098,967 + -3.7 4,098,967 + -2.6 4,098,967 + -2.8 4,098,968 - -0.9 4,098,968 - -1.3 4,098,968 - -0.9 4,099,002 + +0.1 4,099,002 + -1.8 4,099,002 + -1.0 4,099,003 - -2.5 4,099,024 + -1.1 4,099,024 + -1.5 4,099,025 - -2.4 4,099,587 + Pf6N2E2_3514 0.72 -0.8 4,099,587 + Pf6N2E2_3514 0.72 -0.5 4,099,588 - Pf6N2E2_3514 0.72 -2.6 4,099,755 + +0.8 4,099,756 - -2.6 4,099,827 + +0.3 4,099,842 + -2.5 4,100,256 + Pf6N2E2_3515 0.37 -4.1 4,100,256 + Pf6N2E2_3515 0.37 -2.4 4,100,603 - Pf6N2E2_3515 0.69 -1.6 4,100,603 - Pf6N2E2_3515 0.69 -2.9 4,100,635 + Pf6N2E2_3515 0.72 -2.6 4,100,635 + Pf6N2E2_3515 0.72 -0.6 4,100,635 + Pf6N2E2_3515 0.72 -3.8 4,100,635 + Pf6N2E2_3515 0.72 +0.4 4,100,636 - Pf6N2E2_3515 0.72 -1.1 4,100,636 - Pf6N2E2_3515 0.72 -3.9 4,100,636 - Pf6N2E2_3515 0.72 -3.6 4,100,636 - Pf6N2E2_3515 0.72 -2.1 4,100,723 + Pf6N2E2_3515 0.80 -2.7 4,100,723 + Pf6N2E2_3515 0.80 -4.2 4,100,821 + Pf6N2E2_3515 0.89 -2.6 4,100,821 + Pf6N2E2_3515 0.89 -2.6 4,100,821 + Pf6N2E2_3515 0.89 -1.0 4,100,821 + Pf6N2E2_3515 0.89 -2.3 4,100,822 - Pf6N2E2_3515 0.89 -1.6 4,100,822 - Pf6N2E2_3515 0.89 -2.2 4,100,822 - Pf6N2E2_3515 0.89 -3.4 4,100,822 - Pf6N2E2_3515 0.89 -3.3 4,100,822 - Pf6N2E2_3515 0.89 -2.8 4,100,923 + -1.6 4,101,022 + Pf6N2E2_3516 0.49 -0.9 4,101,022 + Pf6N2E2_3516 0.49 -2.8 4,101,023 - Pf6N2E2_3516 0.50 -1.5 4,101,129 + -0.0 4,101,129 + -1.5 4,101,130 - -2.2 4,101,237 + +0.5 4,101,237 + -0.5 4,101,237 + -0.1 4,101,237 + -0.4 4,101,237 + +1.6 4,101,262 + -0.3 4,101,262 + +0.7 4,101,262 + -0.8 4,101,262 + +1.4 4,101,264 + +0.7 4,101,264 + +0.3 4,101,264 + +0.6 4,101,264 + -2.9 4,101,264 + -0.1 4,101,264 + +0.6 4,101,264 + -0.4 4,101,264 + +1.2 4,101,264 + +1.9 4,101,264 + -1.6 4,101,264 + -0.1 4,101,264 + -1.9 4,101,264 + -1.3 4,101,264 + -0.9 4,101,264 + +0.4 4,101,264 + +0.2 4,101,264 + -0.2 4,101,264 + -0.0 4,101,775 + Pf6N2E2_3517 0.53 -0.6 4,101,775 + Pf6N2E2_3517 0.53 -3.2
Or see this region's nucleotide sequence