Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3266

Experiment: Vancomycin 0.24 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3264 and Pf6N2E2_3265 overlap by 14 nucleotidesPf6N2E2_3265 and Pf6N2E2_3266 overlap by 1 nucleotidesPf6N2E2_3266 and Pf6N2E2_3267 are separated by 188 nucleotides Pf6N2E2_3264 - Uncharacterized protein YrbK clustered with lipopolysaccharide transporters, at 3,771,281 to 3,771,850 _3264 Pf6N2E2_3265 - LptA, protein essential for LPS transport across the periplasm, at 3,771,837 to 3,772,388 _3265 Pf6N2E2_3266 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 3,772,388 to 3,773,113 _3266 Pf6N2E2_3267 - RNA polymerase sigma-54 factor RpoN, at 3,773,302 to 3,774,771 _3267 Position (kb) 3772 3773 3774Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 3771.716 kb on + strand, within Pf6N2E2_3264at 3771.716 kb on + strand, within Pf6N2E2_3264at 3771.716 kb on + strand, within Pf6N2E2_3264at 3771.716 kb on + strand, within Pf6N2E2_3264at 3771.825 kb on + strandat 3773.111 kb on + strandat 3773.111 kb on + strandat 3773.112 kb on - strandat 3773.112 kb on - strandat 3773.149 kb on - strandat 3773.155 kb on - strandat 3773.170 kb on + strandat 3773.170 kb on + strandat 3773.171 kb on - strandat 3773.197 kb on + strandat 3773.230 kb on + strandat 3773.230 kb on + strandat 3773.258 kb on + strandat 3773.258 kb on + strandat 3773.266 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml
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3,771,716 + Pf6N2E2_3264 0.76 -4.0
3,771,716 + Pf6N2E2_3264 0.76 -2.5
3,771,716 + Pf6N2E2_3264 0.76 -3.5
3,771,716 + Pf6N2E2_3264 0.76 -3.8
3,771,825 + -2.3
3,773,111 + -1.6
3,773,111 + -3.2
3,773,112 - -1.0
3,773,112 - -2.5
3,773,149 - -0.8
3,773,155 - -4.2
3,773,170 + +0.9
3,773,170 + -0.5
3,773,171 - -3.1
3,773,197 + +0.4
3,773,230 + -1.0
3,773,230 + +0.6
3,773,258 + +3.2
3,773,258 + -0.1
3,773,266 + -2.1

Or see this region's nucleotide sequence