Experiment: Vancomycin 0.24 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf6N2E2_3156 and Pf6N2E2_3157 are separated by 143 nucleotides Pf6N2E2_3157 and Pf6N2E2_3158 overlap by 4 nucleotides Pf6N2E2_3158 and Pf6N2E2_3159 are separated by 736 nucleotides  
        
        Pf6N2E2_3156 - POSSIBLE MEMBRANE PROTEIN, at 3,650,436 to 3,651,080 
         
        _3156 
         
        
        Pf6N2E2_3157 - tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase, at 3,651,224 to 3,651,967 
         
        _3157 
         
        
        Pf6N2E2_3158 - tRNA (5-methoxyuridine) 34 synthase, at 3,651,964 to 3,652,920 
         
        _3158 
         
        
        Pf6N2E2_3159 - tRNA-specific adenosine-34 deaminase (EC 3.5.4.-), at 3,653,657 to 3,654,154 
         
        _3159 
         Position (kb)  
3651 
 
3652 
 
3653 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 at 3651.038 kb on + strand at 3651.038 kb on + strand at 3651.038 kb on + strand at 3651.039 kb on - strand at 3651.078 kb on + strand at 3651.078 kb on + strand at 3651.078 kb on + strand at 3651.078 kb on + strand at 3651.078 kb on + strand at 3651.079 kb on - strand at 3651.079 kb on - strand at 3651.079 kb on - strand at 3651.079 kb on - strand at 3651.112 kb on + strand at 3651.113 kb on - strand at 3651.113 kb on - strand at 3651.407 kb on + strand, within Pf6N2E2_3157 at 3651.408 kb on - strand, within Pf6N2E2_3157 at 3651.408 kb on - strand, within Pf6N2E2_3157 at 3651.494 kb on - strand, within Pf6N2E2_3157 at 3651.527 kb on + strand, within Pf6N2E2_3157 at 3651.678 kb on + strand, within Pf6N2E2_3157 at 3651.678 kb on + strand, within Pf6N2E2_3157 at 3651.678 kb on + strand, within Pf6N2E2_3157 at 3651.679 kb on - strand, within Pf6N2E2_3157 at 3651.679 kb on - strand, within Pf6N2E2_3157 at 3651.870 kb on + strand, within Pf6N2E2_3157 at 3651.870 kb on + strand, within Pf6N2E2_3157 at 3651.871 kb on - strand, within Pf6N2E2_3157 at 3651.871 kb on - strand, within Pf6N2E2_3157 at 3651.937 kb on + strand at 3651.937 kb on + strand at 3651.937 kb on + strand at 3651.938 kb on - strand at 3651.938 kb on - strand at 3651.938 kb on - strand at 3652.256 kb on + strand, within Pf6N2E2_3158 at 3652.256 kb on + strand, within Pf6N2E2_3158 at 3652.256 kb on + strand, within Pf6N2E2_3158 at 3652.257 kb on - strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.357 kb on + strand, within Pf6N2E2_3158 at 3652.358 kb on - strand, within Pf6N2E2_3158 at 3652.358 kb on - strand, within Pf6N2E2_3158 at 3652.358 kb on - strand, within Pf6N2E2_3158 at 3652.358 kb on - strand, within Pf6N2E2_3158 at 3652.358 kb on - strand, within Pf6N2E2_3158 at 3652.358 kb on - strand, within Pf6N2E2_3158 at 3652.550 kb on - strand, within Pf6N2E2_3158 at 3652.822 kb on + strand, within Pf6N2E2_3158 at 3652.823 kb on - strand, within Pf6N2E2_3158 at 3652.823 kb on - strand, within Pf6N2E2_3158 at 3652.823 kb on - strand, within Pf6N2E2_3158 at 3652.823 kb on - strand, within Pf6N2E2_3158 at 3652.823 kb on - strand, within Pf6N2E2_3158 at 3652.823 kb on - strand, within Pf6N2E2_3158 at 3652.823 kb on - strand, within Pf6N2E2_3158 at 3652.919 kb on - strand at 3652.933 kb on + strand at 3652.934 kb on - strand at 3652.945 kb on + strand at 3652.945 kb on + strand at 3652.945 kb on + strand at 3652.946 kb on - strand at 3652.946 kb on - strand at 3652.946 kb on - strand at 3653.483 kb on + strand at 3653.602 kb on + strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Vancomycin 0.24 mg/ml             remove   3,651,038   +        +1.3   3,651,038   +        -0.9   3,651,038   +        +1.6   3,651,039   -        -0.2   3,651,078   +        +0.4   3,651,078   +        -0.4   3,651,078   +        -1.0   3,651,078   +        -1.4   3,651,078   +        +0.1   3,651,079   -        -0.2   3,651,079   -        +0.1   3,651,079   -        +0.9   3,651,079   -        +0.1   3,651,112   +        +0.3   3,651,113   -        +1.3   3,651,113   -        +0.8   3,651,407   +    Pf6N2E2_3157   0.25  -0.6   3,651,408   -    Pf6N2E2_3157   0.25  +0.9   3,651,408   -    Pf6N2E2_3157   0.25  -1.3   3,651,494   -    Pf6N2E2_3157   0.36  +0.1   3,651,527   +    Pf6N2E2_3157   0.41  +0.7   3,651,678   +    Pf6N2E2_3157   0.61  -0.9   3,651,678   +    Pf6N2E2_3157   0.61  -0.7   3,651,678   +    Pf6N2E2_3157   0.61  +0.1   3,651,679   -    Pf6N2E2_3157   0.61  -1.4   3,651,679   -    Pf6N2E2_3157   0.61  -0.3   3,651,870   +    Pf6N2E2_3157   0.87  -0.8   3,651,870   +    Pf6N2E2_3157   0.87  -0.8   3,651,871   -    Pf6N2E2_3157   0.87  -0.4   3,651,871   -    Pf6N2E2_3157   0.87  +0.8   3,651,937   +        +0.0   3,651,937   +        -0.4   3,651,937   +        +0.0   3,651,938   -        -0.2   3,651,938   -        -1.1   3,651,938   -        +0.5   3,652,256   +    Pf6N2E2_3158   0.31  -1.4   3,652,256   +    Pf6N2E2_3158   0.31  +1.3   3,652,256   +    Pf6N2E2_3158   0.31  -0.3   3,652,257   -    Pf6N2E2_3158   0.31  -1.9   3,652,357   +    Pf6N2E2_3158   0.41  -0.8   3,652,357   +    Pf6N2E2_3158   0.41  +1.7   3,652,357   +    Pf6N2E2_3158   0.41  -1.5   3,652,357   +    Pf6N2E2_3158   0.41  -1.1   3,652,357   +    Pf6N2E2_3158   0.41  +0.0   3,652,357   +    Pf6N2E2_3158   0.41  +1.8   3,652,357   +    Pf6N2E2_3158   0.41  +0.2   3,652,357   +    Pf6N2E2_3158   0.41  +0.0   3,652,357   +    Pf6N2E2_3158   0.41  -0.2   3,652,357   +    Pf6N2E2_3158   0.41  -0.6   3,652,357   +    Pf6N2E2_3158   0.41  -0.8   3,652,357   +    Pf6N2E2_3158   0.41  -1.1   3,652,357   +    Pf6N2E2_3158   0.41  -0.2   3,652,357   +    Pf6N2E2_3158   0.41  +0.5   3,652,357   +    Pf6N2E2_3158   0.41  -0.8   3,652,358   -    Pf6N2E2_3158   0.41  -1.1   3,652,358   -    Pf6N2E2_3158   0.41  -0.3   3,652,358   -    Pf6N2E2_3158   0.41  -1.2   3,652,358   -    Pf6N2E2_3158   0.41  -0.7   3,652,358   -    Pf6N2E2_3158   0.41  +0.3   3,652,358   -    Pf6N2E2_3158   0.41  +0.4   3,652,550   -    Pf6N2E2_3158   0.61  -0.4   3,652,822   +    Pf6N2E2_3158   0.90  -1.6   3,652,823   -    Pf6N2E2_3158   0.90  +0.1   3,652,823   -    Pf6N2E2_3158   0.90  -0.3   3,652,823   -    Pf6N2E2_3158   0.90  -0.9   3,652,823   -    Pf6N2E2_3158   0.90  -1.8   3,652,823   -    Pf6N2E2_3158   0.90  -3.4   3,652,823   -    Pf6N2E2_3158   0.90  -0.4   3,652,823   -    Pf6N2E2_3158   0.90  -1.3   3,652,919   -        -0.2   3,652,933   +        -0.7   3,652,934   -        +0.2   3,652,945   +        +0.5   3,652,945   +        -1.6   3,652,945   +        +0.6   3,652,946   -        +0.0   3,652,946   -        -2.6   3,652,946   -        +0.4   3,653,483   +        +0.7   3,653,602   +        -0.5 
 
Or see this region's nucleotide sequence