Experiment: Vancomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3039 and Pf6N2E2_3040 overlap by 1 nucleotides Pf6N2E2_3040 and Pf6N2E2_3041 overlap by 13 nucleotides Pf6N2E2_3041 and Pf6N2E2_3042 overlap by 4 nucleotides Pf6N2E2_3042 and Pf6N2E2_3043 are separated by 43 nucleotides
Pf6N2E2_3039 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 3,523,850 to 3,524,527
_3039
Pf6N2E2_3040 - 5-nucleotidase SurE (EC 3.1.3.5), at 3,524,527 to 3,525,276
_3040
Pf6N2E2_3041 - tRNA pseudouridine 13 synthase (EC 4.2.1.-), at 3,525,264 to 3,526,322
_3041
Pf6N2E2_3042 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 3,526,319 to 3,526,792
_3042
Pf6N2E2_3043 - S-formylglutathione hydrolase (EC 3.1.2.12), at 3,526,836 to 3,527,681
_3043
Position (kb)
3525
3526
3527 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.396 kb on - strand, within Pf6N2E2_3039 at 3524.442 kb on + strand, within Pf6N2E2_3039 at 3524.443 kb on - strand, within Pf6N2E2_3039 at 3524.580 kb on + strand at 3524.581 kb on - strand at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.972 kb on + strand, within Pf6N2E2_3040 at 3524.973 kb on - strand, within Pf6N2E2_3040 at 3524.973 kb on - strand, within Pf6N2E2_3040 at 3525.009 kb on + strand, within Pf6N2E2_3040 at 3525.082 kb on - strand, within Pf6N2E2_3040 at 3525.113 kb on + strand, within Pf6N2E2_3040 at 3525.219 kb on - strand at 3525.219 kb on - strand at 3525.270 kb on + strand at 3525.270 kb on + strand at 3525.271 kb on - strand at 3525.271 kb on - strand at 3525.326 kb on - strand at 3525.453 kb on + strand, within Pf6N2E2_3041 at 3525.454 kb on - strand, within Pf6N2E2_3041 at 3525.454 kb on - strand, within Pf6N2E2_3041 at 3525.747 kb on + strand, within Pf6N2E2_3041 at 3525.747 kb on + strand, within Pf6N2E2_3041 at 3525.748 kb on - strand, within Pf6N2E2_3041 at 3525.748 kb on - strand, within Pf6N2E2_3041 at 3525.841 kb on + strand, within Pf6N2E2_3041 at 3525.842 kb on - strand, within Pf6N2E2_3041 at 3525.926 kb on + strand, within Pf6N2E2_3041 at 3525.926 kb on + strand, within Pf6N2E2_3041 at 3526.096 kb on + strand, within Pf6N2E2_3041 at 3526.264 kb on - strand at 3526.828 kb on + strand at 3526.829 kb on - strand at 3526.845 kb on + strand at 3526.845 kb on + strand at 3526.846 kb on - strand at 3526.900 kb on + strand at 3526.900 kb on + strand at 3526.901 kb on - strand at 3526.901 kb on - strand at 3526.901 kb on - strand at 3526.901 kb on - strand at 3526.901 kb on - strand at 3526.901 kb on - strand at 3526.901 kb on - strand at 3526.913 kb on - strand at 3527.308 kb on + strand, within Pf6N2E2_3043 at 3527.308 kb on + strand, within Pf6N2E2_3043 at 3527.308 kb on + strand, within Pf6N2E2_3043 at 3527.308 kb on + strand, within Pf6N2E2_3043 at 3527.308 kb on + strand, within Pf6N2E2_3043 at 3527.308 kb on + strand, within Pf6N2E2_3043 at 3527.309 kb on - strand, within Pf6N2E2_3043 at 3527.309 kb on - strand, within Pf6N2E2_3043 at 3527.309 kb on - strand, within Pf6N2E2_3043 at 3527.309 kb on - strand, within Pf6N2E2_3043
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml remove 3,524,298 + Pf6N2E2_3039 0.66 -2.0 3,524,298 + Pf6N2E2_3039 0.66 -3.0 3,524,298 + Pf6N2E2_3039 0.66 -3.2 3,524,298 + Pf6N2E2_3039 0.66 -1.0 3,524,298 + Pf6N2E2_3039 0.66 -0.6 3,524,298 + Pf6N2E2_3039 0.66 -2.3 3,524,298 + Pf6N2E2_3039 0.66 -1.9 3,524,298 + Pf6N2E2_3039 0.66 -3.9 3,524,298 + Pf6N2E2_3039 0.66 -3.4 3,524,298 + Pf6N2E2_3039 0.66 -1.3 3,524,299 - Pf6N2E2_3039 0.66 -2.0 3,524,299 - Pf6N2E2_3039 0.66 -1.2 3,524,299 - Pf6N2E2_3039 0.66 -3.2 3,524,299 - Pf6N2E2_3039 0.66 -4.4 3,524,299 - Pf6N2E2_3039 0.66 -1.2 3,524,299 - Pf6N2E2_3039 0.66 -1.4 3,524,299 - Pf6N2E2_3039 0.66 -3.0 3,524,299 - Pf6N2E2_3039 0.66 -3.3 3,524,396 - Pf6N2E2_3039 0.81 +0.4 3,524,442 + Pf6N2E2_3039 0.87 -1.8 3,524,443 - Pf6N2E2_3039 0.87 -1.7 3,524,580 + -2.1 3,524,581 - -1.8 3,524,612 + Pf6N2E2_3040 0.11 -2.0 3,524,612 + Pf6N2E2_3040 0.11 +0.2 3,524,612 + Pf6N2E2_3040 0.11 -1.6 3,524,612 + Pf6N2E2_3040 0.11 -1.9 3,524,612 + Pf6N2E2_3040 0.11 -1.7 3,524,612 + Pf6N2E2_3040 0.11 -1.6 3,524,612 + Pf6N2E2_3040 0.11 -2.3 3,524,612 + Pf6N2E2_3040 0.11 -1.6 3,524,613 - Pf6N2E2_3040 0.11 -0.4 3,524,613 - Pf6N2E2_3040 0.11 -1.8 3,524,613 - Pf6N2E2_3040 0.11 +0.0 3,524,613 - Pf6N2E2_3040 0.11 -0.2 3,524,613 - Pf6N2E2_3040 0.11 -3.2 3,524,613 - Pf6N2E2_3040 0.11 -2.4 3,524,613 - Pf6N2E2_3040 0.11 -1.0 3,524,613 - Pf6N2E2_3040 0.11 -2.3 3,524,613 - Pf6N2E2_3040 0.11 -0.9 3,524,972 + Pf6N2E2_3040 0.59 -1.9 3,524,973 - Pf6N2E2_3040 0.59 +0.0 3,524,973 - Pf6N2E2_3040 0.59 +1.3 3,525,009 + Pf6N2E2_3040 0.64 -1.4 3,525,082 - Pf6N2E2_3040 0.74 +1.8 3,525,113 + Pf6N2E2_3040 0.78 -2.1 3,525,219 - +1.0 3,525,219 - +0.8 3,525,270 + -2.6 3,525,270 + -1.6 3,525,271 - +1.6 3,525,271 - +2.0 3,525,326 - +2.7 3,525,453 + Pf6N2E2_3041 0.18 +2.5 3,525,454 - Pf6N2E2_3041 0.18 +1.0 3,525,454 - Pf6N2E2_3041 0.18 -1.4 3,525,747 + Pf6N2E2_3041 0.46 +1.7 3,525,747 + Pf6N2E2_3041 0.46 +1.2 3,525,748 - Pf6N2E2_3041 0.46 +0.3 3,525,748 - Pf6N2E2_3041 0.46 +0.3 3,525,841 + Pf6N2E2_3041 0.54 +1.9 3,525,842 - Pf6N2E2_3041 0.55 +1.5 3,525,926 + Pf6N2E2_3041 0.63 +2.2 3,525,926 + Pf6N2E2_3041 0.63 +2.3 3,526,096 + Pf6N2E2_3041 0.79 +2.8 3,526,264 - +2.0 3,526,828 + +0.6 3,526,829 - +1.7 3,526,845 + +0.8 3,526,845 + -0.2 3,526,846 - +0.7 3,526,900 + +1.3 3,526,900 + +0.8 3,526,901 - +0.9 3,526,901 - +0.6 3,526,901 - +0.5 3,526,901 - +1.2 3,526,901 - +1.7 3,526,901 - -0.2 3,526,901 - +1.9 3,526,913 - +1.6 3,527,308 + Pf6N2E2_3043 0.56 +1.2 3,527,308 + Pf6N2E2_3043 0.56 +0.4 3,527,308 + Pf6N2E2_3043 0.56 +0.9 3,527,308 + Pf6N2E2_3043 0.56 +0.2 3,527,308 + Pf6N2E2_3043 0.56 +0.0 3,527,308 + Pf6N2E2_3043 0.56 -0.0 3,527,309 - Pf6N2E2_3043 0.56 +0.9 3,527,309 - Pf6N2E2_3043 0.56 +0.7 3,527,309 - Pf6N2E2_3043 0.56 +1.1 3,527,309 - Pf6N2E2_3043 0.56 +1.2
Or see this region's nucleotide sequence