Experiment: Vancomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2577 and Pf6N2E2_2578 are separated by 3 nucleotides Pf6N2E2_2578 and Pf6N2E2_2579 are separated by 4 nucleotides Pf6N2E2_2579 and Pf6N2E2_2580 are separated by 23 nucleotides Pf6N2E2_2580 and Pf6N2E2_2581 overlap by 1 nucleotides
Pf6N2E2_2577 - Flagellar biosynthesis protein FlhB, at 3,036,721 to 3,037,857
_2577
Pf6N2E2_2578 - Flagellar biosynthesis protein FliR, at 3,037,861 to 3,038,643
_2578
Pf6N2E2_2579 - Flagellar biosynthesis protein FliQ, at 3,038,648 to 3,038,917
_2579
Pf6N2E2_2580 - Flagellar biosynthesis protein FliP, at 3,038,941 to 3,039,690
_2580
Pf6N2E2_2581 - Flagellar biosynthesis protein FliQ, at 3,039,690 to 3,040,127
_2581
Position (kb)
3038
3039 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.710 kb on - strand, within Pf6N2E2_2577 at 3037.710 kb on - strand, within Pf6N2E2_2577 at 3037.710 kb on - strand, within Pf6N2E2_2577 at 3037.885 kb on + strand at 3037.886 kb on - strand at 3038.077 kb on + strand, within Pf6N2E2_2578 at 3038.286 kb on + strand, within Pf6N2E2_2578 at 3038.504 kb on + strand, within Pf6N2E2_2578 at 3038.607 kb on + strand at 3038.669 kb on - strand at 3038.669 kb on - strand at 3038.677 kb on - strand, within Pf6N2E2_2579 at 3038.677 kb on - strand, within Pf6N2E2_2579 at 3038.691 kb on + strand, within Pf6N2E2_2579 at 3038.691 kb on + strand, within Pf6N2E2_2579 at 3038.691 kb on + strand, within Pf6N2E2_2579 at 3038.691 kb on + strand, within Pf6N2E2_2579 at 3038.692 kb on - strand, within Pf6N2E2_2579 at 3038.692 kb on - strand, within Pf6N2E2_2579 at 3038.692 kb on - strand, within Pf6N2E2_2579 at 3038.692 kb on - strand, within Pf6N2E2_2579 at 3038.709 kb on - strand, within Pf6N2E2_2579 at 3038.861 kb on + strand, within Pf6N2E2_2579 at 3038.861 kb on + strand, within Pf6N2E2_2579 at 3038.862 kb on - strand, within Pf6N2E2_2579 at 3038.903 kb on + strand at 3038.903 kb on + strand at 3038.904 kb on - strand at 3038.917 kb on + strand at 3038.942 kb on + strand at 3038.943 kb on - strand at 3039.207 kb on + strand, within Pf6N2E2_2580 at 3039.335 kb on + strand, within Pf6N2E2_2580 at 3039.335 kb on + strand, within Pf6N2E2_2580 at 3039.336 kb on - strand, within Pf6N2E2_2580 at 3039.336 kb on - strand, within Pf6N2E2_2580 at 3039.569 kb on + strand, within Pf6N2E2_2580 at 3039.569 kb on + strand, within Pf6N2E2_2580 at 3039.692 kb on - strand at 3039.692 kb on - strand at 3039.855 kb on + strand, within Pf6N2E2_2581
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml remove 3,037,709 + Pf6N2E2_2577 0.87 -2.4 3,037,709 + Pf6N2E2_2577 0.87 -0.5 3,037,709 + Pf6N2E2_2577 0.87 -1.2 3,037,709 + Pf6N2E2_2577 0.87 -1.6 3,037,709 + Pf6N2E2_2577 0.87 +0.1 3,037,709 + Pf6N2E2_2577 0.87 +2.3 3,037,709 + Pf6N2E2_2577 0.87 -0.1 3,037,710 - Pf6N2E2_2577 0.87 -0.7 3,037,710 - Pf6N2E2_2577 0.87 +0.1 3,037,710 - Pf6N2E2_2577 0.87 -2.3 3,037,885 + -1.7 3,037,886 - +0.1 3,038,077 + Pf6N2E2_2578 0.28 -1.4 3,038,286 + Pf6N2E2_2578 0.54 -1.6 3,038,504 + Pf6N2E2_2578 0.82 -2.9 3,038,607 + -1.3 3,038,669 - -1.4 3,038,669 - -1.0 3,038,677 - Pf6N2E2_2579 0.11 -0.8 3,038,677 - Pf6N2E2_2579 0.11 -0.3 3,038,691 + Pf6N2E2_2579 0.16 +0.4 3,038,691 + Pf6N2E2_2579 0.16 -1.0 3,038,691 + Pf6N2E2_2579 0.16 -1.6 3,038,691 + Pf6N2E2_2579 0.16 -1.3 3,038,692 - Pf6N2E2_2579 0.16 -0.0 3,038,692 - Pf6N2E2_2579 0.16 -1.5 3,038,692 - Pf6N2E2_2579 0.16 -0.8 3,038,692 - Pf6N2E2_2579 0.16 -1.2 3,038,709 - Pf6N2E2_2579 0.23 -2.5 3,038,861 + Pf6N2E2_2579 0.79 -0.8 3,038,861 + Pf6N2E2_2579 0.79 -0.0 3,038,862 - Pf6N2E2_2579 0.79 -0.7 3,038,903 + -2.4 3,038,903 + -0.3 3,038,904 - -2.2 3,038,917 + -1.0 3,038,942 + -2.0 3,038,943 - -1.4 3,039,207 + Pf6N2E2_2580 0.35 -1.2 3,039,335 + Pf6N2E2_2580 0.53 -0.8 3,039,335 + Pf6N2E2_2580 0.53 -1.1 3,039,336 - Pf6N2E2_2580 0.53 -1.3 3,039,336 - Pf6N2E2_2580 0.53 -0.0 3,039,569 + Pf6N2E2_2580 0.84 -1.0 3,039,569 + Pf6N2E2_2580 0.84 -0.5 3,039,692 - -0.8 3,039,692 - -1.9 3,039,855 + Pf6N2E2_2581 0.38 +0.4
Or see this region's nucleotide sequence