Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_221

Experiment: Vancomycin 0.24 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_220 and Pf6N2E2_221 are separated by 112 nucleotidesPf6N2E2_221 and Pf6N2E2_222 are separated by 2 nucleotides Pf6N2E2_220 - Sensory histidine kinase BaeS, at 277,708 to 279,126 _220 Pf6N2E2_221 - RND efflux system, membrane fusion protein CmeA, at 279,239 to 280,432 _221 Pf6N2E2_222 - RND efflux system, inner membrane transporter CmeB, at 280,435 to 283,581 _222 Position (kb) 279 280 281Strain fitness (log2 ratio) -2 -1 0 1 2 3at 278.400 kb on + strand, within Pf6N2E2_220at 278.402 kb on + strand, within Pf6N2E2_220at 278.403 kb on - strand, within Pf6N2E2_220at 278.403 kb on - strand, within Pf6N2E2_220at 278.474 kb on + strand, within Pf6N2E2_220at 278.613 kb on - strand, within Pf6N2E2_220at 278.979 kb on - strand, within Pf6N2E2_220at 279.064 kb on - strandat 279.128 kb on + strandat 279.238 kb on + strandat 279.238 kb on + strandat 279.239 kb on - strandat 279.444 kb on + strandat 279.522 kb on - strand, within Pf6N2E2_221at 279.542 kb on + strand, within Pf6N2E2_221at 279.542 kb on + strand, within Pf6N2E2_221at 279.542 kb on + strand, within Pf6N2E2_221at 279.542 kb on + strand, within Pf6N2E2_221at 279.542 kb on + strand, within Pf6N2E2_221at 279.543 kb on - strand, within Pf6N2E2_221at 279.758 kb on + strand, within Pf6N2E2_221at 279.758 kb on + strand, within Pf6N2E2_221at 279.758 kb on + strand, within Pf6N2E2_221at 279.759 kb on - strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.879 kb on - strand, within Pf6N2E2_221at 279.882 kb on + strand, within Pf6N2E2_221at 279.882 kb on + strand, within Pf6N2E2_221at 279.882 kb on + strand, within Pf6N2E2_221at 279.882 kb on + strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.945 kb on + strand, within Pf6N2E2_221at 279.945 kb on + strand, within Pf6N2E2_221at 279.946 kb on - strand, within Pf6N2E2_221at 279.946 kb on - strand, within Pf6N2E2_221at 280.004 kb on + strand, within Pf6N2E2_221at 280.004 kb on + strand, within Pf6N2E2_221at 280.004 kb on + strand, within Pf6N2E2_221at 280.005 kb on - strand, within Pf6N2E2_221at 280.005 kb on - strand, within Pf6N2E2_221at 280.005 kb on - strand, within Pf6N2E2_221at 280.005 kb on - strand, within Pf6N2E2_221at 280.118 kb on + strand, within Pf6N2E2_221at 280.118 kb on + strand, within Pf6N2E2_221at 280.118 kb on + strand, within Pf6N2E2_221at 280.119 kb on - strand, within Pf6N2E2_221at 280.119 kb on - strand, within Pf6N2E2_221at 280.119 kb on - strand, within Pf6N2E2_221at 280.579 kb on + strandat 280.579 kb on + strandat 280.663 kb on + strandat 280.663 kb on + strandat 280.663 kb on + strandat 280.663 kb on + strandat 280.663 kb on + strandat 280.663 kb on + strandat 280.663 kb on + strandat 280.664 kb on - strandat 280.664 kb on - strandat 280.664 kb on - strandat 280.664 kb on - strandat 280.664 kb on - strandat 280.664 kb on - strandat 280.664 kb on - strandat 280.904 kb on - strand, within Pf6N2E2_222at 280.904 kb on - strand, within Pf6N2E2_222at 280.904 kb on - strand, within Pf6N2E2_222at 281.258 kb on - strand, within Pf6N2E2_222at 281.258 kb on - strand, within Pf6N2E2_222at 281.383 kb on + strand, within Pf6N2E2_222at 281.383 kb on + strand, within Pf6N2E2_222

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Per-strain Table

Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml
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278,400 + Pf6N2E2_220 0.49 -2.4
278,402 + Pf6N2E2_220 0.49 +0.7
278,403 - Pf6N2E2_220 0.49 +1.0
278,403 - Pf6N2E2_220 0.49 +0.6
278,474 + Pf6N2E2_220 0.54 -0.1
278,613 - Pf6N2E2_220 0.64 -0.4
278,979 - Pf6N2E2_220 0.90 -2.7
279,064 - +0.9
279,128 + +1.5
279,238 + +0.2
279,238 + -0.1
279,239 - -0.1
279,444 + -0.1
279,522 - Pf6N2E2_221 0.24 +0.1
279,542 + Pf6N2E2_221 0.25 -0.7
279,542 + Pf6N2E2_221 0.25 -0.6
279,542 + Pf6N2E2_221 0.25 -2.5
279,542 + Pf6N2E2_221 0.25 -0.6
279,542 + Pf6N2E2_221 0.25 +0.3
279,543 - Pf6N2E2_221 0.25 -0.5
279,758 + Pf6N2E2_221 0.43 -0.4
279,758 + Pf6N2E2_221 0.43 +1.4
279,758 + Pf6N2E2_221 0.43 -1.7
279,759 - Pf6N2E2_221 0.44 -0.5
279,878 + Pf6N2E2_221 0.54 +0.5
279,878 + Pf6N2E2_221 0.54 -1.4
279,878 + Pf6N2E2_221 0.54 -0.7
279,878 + Pf6N2E2_221 0.54 +0.5
279,878 + Pf6N2E2_221 0.54 -0.0
279,878 + Pf6N2E2_221 0.54 -0.4
279,878 + Pf6N2E2_221 0.54 -1.0
279,879 - Pf6N2E2_221 0.54 -1.2
279,882 + Pf6N2E2_221 0.54 -1.1
279,882 + Pf6N2E2_221 0.54 -0.1
279,882 + Pf6N2E2_221 0.54 -0.5
279,882 + Pf6N2E2_221 0.54 +0.4
279,883 - Pf6N2E2_221 0.54 -1.4
279,883 - Pf6N2E2_221 0.54 +1.7
279,883 - Pf6N2E2_221 0.54 +1.5
279,883 - Pf6N2E2_221 0.54 -0.8
279,883 - Pf6N2E2_221 0.54 +0.9
279,883 - Pf6N2E2_221 0.54 +0.0
279,883 - Pf6N2E2_221 0.54 -1.9
279,945 + Pf6N2E2_221 0.59 -0.5
279,945 + Pf6N2E2_221 0.59 -1.5
279,946 - Pf6N2E2_221 0.59 -0.8
279,946 - Pf6N2E2_221 0.59 +0.2
280,004 + Pf6N2E2_221 0.64 +1.0
280,004 + Pf6N2E2_221 0.64 -1.3
280,004 + Pf6N2E2_221 0.64 -0.1
280,005 - Pf6N2E2_221 0.64 -1.0
280,005 - Pf6N2E2_221 0.64 -0.2
280,005 - Pf6N2E2_221 0.64 +1.1
280,005 - Pf6N2E2_221 0.64 +0.2
280,118 + Pf6N2E2_221 0.74 -0.6
280,118 + Pf6N2E2_221 0.74 -0.3
280,118 + Pf6N2E2_221 0.74 +0.2
280,119 - Pf6N2E2_221 0.74 -1.1
280,119 - Pf6N2E2_221 0.74 +2.8
280,119 - Pf6N2E2_221 0.74 -0.8
280,579 + -1.8
280,579 + +0.3
280,663 + -0.7
280,663 + +1.2
280,663 + +0.0
280,663 + +0.3
280,663 + -0.1
280,663 + +1.7
280,663 + -0.7
280,664 - -0.4
280,664 - -0.3
280,664 - +1.5
280,664 - -1.0
280,664 - -0.7
280,664 - +0.1
280,664 - -0.5
280,904 - Pf6N2E2_222 0.15 -0.9
280,904 - Pf6N2E2_222 0.15 -0.5
280,904 - Pf6N2E2_222 0.15 +0.5
281,258 - Pf6N2E2_222 0.26 -0.1
281,258 - Pf6N2E2_222 0.26 -0.3
281,383 + Pf6N2E2_222 0.30 +1.0
281,383 + Pf6N2E2_222 0.30 -0.7

Or see this region's nucleotide sequence