Experiment: Vancomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1134 and Pf6N2E2_1135 are separated by 285 nucleotides Pf6N2E2_1135 and Pf6N2E2_1136 are separated by 45 nucleotides Pf6N2E2_1136 and Pf6N2E2_1137 are separated by 121 nucleotides
Pf6N2E2_1134 - Ribosomal protein S3AE, at 1,331,338 to 1,331,739
_1134
Pf6N2E2_1135 - COGs COG3558, at 1,332,025 to 1,332,504
_1135
Pf6N2E2_1136 - Transcriptional regulator, TetR family, at 1,332,550 to 1,333,113
_1136
Pf6N2E2_1137 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 1,333,235 to 1,334,578
_1137
Position (kb)
1332
1333
1334 Strain fitness (log2 ratio)
-2
-1
0
1 at 1331.950 kb on + strand at 1332.014 kb on - strand at 1332.026 kb on + strand at 1332.026 kb on + strand at 1332.140 kb on + strand, within Pf6N2E2_1135 at 1332.140 kb on + strand, within Pf6N2E2_1135 at 1332.140 kb on + strand, within Pf6N2E2_1135 at 1332.140 kb on + strand, within Pf6N2E2_1135 at 1332.141 kb on - strand, within Pf6N2E2_1135 at 1332.141 kb on - strand, within Pf6N2E2_1135 at 1332.141 kb on - strand, within Pf6N2E2_1135 at 1332.182 kb on + strand, within Pf6N2E2_1135 at 1332.222 kb on - strand, within Pf6N2E2_1135 at 1332.222 kb on - strand, within Pf6N2E2_1135 at 1332.222 kb on - strand, within Pf6N2E2_1135 at 1332.228 kb on - strand, within Pf6N2E2_1135 at 1332.713 kb on + strand, within Pf6N2E2_1136 at 1332.978 kb on - strand, within Pf6N2E2_1136 at 1333.048 kb on - strand, within Pf6N2E2_1136 at 1333.194 kb on + strand at 1333.194 kb on + strand at 1333.195 kb on - strand at 1333.195 kb on - strand at 1333.234 kb on + strand at 1333.235 kb on - strand at 1333.235 kb on - strand at 1333.376 kb on + strand, within Pf6N2E2_1137 at 1333.376 kb on + strand, within Pf6N2E2_1137 at 1333.377 kb on - strand, within Pf6N2E2_1137 at 1333.377 kb on - strand, within Pf6N2E2_1137 at 1333.377 kb on - strand, within Pf6N2E2_1137 at 1333.391 kb on + strand, within Pf6N2E2_1137 at 1333.392 kb on - strand, within Pf6N2E2_1137 at 1333.589 kb on + strand, within Pf6N2E2_1137 at 1333.590 kb on - strand, within Pf6N2E2_1137 at 1333.590 kb on - strand, within Pf6N2E2_1137 at 1333.688 kb on - strand, within Pf6N2E2_1137 at 1333.688 kb on - strand, within Pf6N2E2_1137 at 1333.688 kb on - strand, within Pf6N2E2_1137 at 1333.694 kb on + strand, within Pf6N2E2_1137 at 1333.890 kb on - strand, within Pf6N2E2_1137 at 1333.899 kb on - strand, within Pf6N2E2_1137 at 1333.899 kb on - strand, within Pf6N2E2_1137 at 1333.899 kb on - strand, within Pf6N2E2_1137 at 1333.899 kb on - strand, within Pf6N2E2_1137 at 1334.039 kb on + strand, within Pf6N2E2_1137 at 1334.039 kb on + strand, within Pf6N2E2_1137 at 1334.040 kb on - strand, within Pf6N2E2_1137 at 1334.040 kb on - strand, within Pf6N2E2_1137 at 1334.040 kb on - strand, within Pf6N2E2_1137
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml remove 1,331,950 + +0.7 1,332,014 - -0.9 1,332,026 + -1.4 1,332,026 + -0.8 1,332,140 + Pf6N2E2_1135 0.24 +0.4 1,332,140 + Pf6N2E2_1135 0.24 -0.7 1,332,140 + Pf6N2E2_1135 0.24 -0.4 1,332,140 + Pf6N2E2_1135 0.24 +0.0 1,332,141 - Pf6N2E2_1135 0.24 +0.4 1,332,141 - Pf6N2E2_1135 0.24 -1.3 1,332,141 - Pf6N2E2_1135 0.24 +0.9 1,332,182 + Pf6N2E2_1135 0.33 +1.0 1,332,222 - Pf6N2E2_1135 0.41 -1.0 1,332,222 - Pf6N2E2_1135 0.41 +1.1 1,332,222 - Pf6N2E2_1135 0.41 -0.2 1,332,228 - Pf6N2E2_1135 0.42 -0.1 1,332,713 + Pf6N2E2_1136 0.29 +0.1 1,332,978 - Pf6N2E2_1136 0.76 -1.9 1,333,048 - Pf6N2E2_1136 0.88 -2.7 1,333,194 + -2.3 1,333,194 + -0.1 1,333,195 - +0.5 1,333,195 - -2.2 1,333,234 + +1.3 1,333,235 - -1.1 1,333,235 - -0.1 1,333,376 + Pf6N2E2_1137 0.10 +0.8 1,333,376 + Pf6N2E2_1137 0.10 -1.1 1,333,377 - Pf6N2E2_1137 0.11 +0.5 1,333,377 - Pf6N2E2_1137 0.11 +0.3 1,333,377 - Pf6N2E2_1137 0.11 -0.7 1,333,391 + Pf6N2E2_1137 0.12 +0.7 1,333,392 - Pf6N2E2_1137 0.12 +1.0 1,333,589 + Pf6N2E2_1137 0.26 +0.2 1,333,590 - Pf6N2E2_1137 0.26 +1.1 1,333,590 - Pf6N2E2_1137 0.26 -0.1 1,333,688 - Pf6N2E2_1137 0.34 -0.8 1,333,688 - Pf6N2E2_1137 0.34 -1.0 1,333,688 - Pf6N2E2_1137 0.34 +1.2 1,333,694 + Pf6N2E2_1137 0.34 -0.7 1,333,890 - Pf6N2E2_1137 0.49 -0.5 1,333,899 - Pf6N2E2_1137 0.49 -0.4 1,333,899 - Pf6N2E2_1137 0.49 -0.4 1,333,899 - Pf6N2E2_1137 0.49 -0.7 1,333,899 - Pf6N2E2_1137 0.49 -0.7 1,334,039 + Pf6N2E2_1137 0.60 -0.7 1,334,039 + Pf6N2E2_1137 0.60 +0.9 1,334,040 - Pf6N2E2_1137 0.60 -0.3 1,334,040 - Pf6N2E2_1137 0.60 +0.4 1,334,040 - Pf6N2E2_1137 0.60 -0.2
Or see this region's nucleotide sequence