Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05847

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05846 and H281DRAFT_05847 are separated by 155 nucleotidesH281DRAFT_05847 and H281DRAFT_05848 are separated by 347 nucleotidesH281DRAFT_05848 and H281DRAFT_05849 are separated by 158 nucleotides H281DRAFT_05846: H281DRAFT_05846 - malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+), at 122,903 to 125,185 _05846 H281DRAFT_05847: H281DRAFT_05847 - indolepyruvate ferredoxin oxidoreductase, at 125,341 to 128,940 _05847 H281DRAFT_05848: H281DRAFT_05848 - hypothetical protein, at 129,288 to 129,485 _05848 H281DRAFT_05849: H281DRAFT_05849 - 4-hydroxyphenylpyruvate dioxygenase, at 129,644 to 130,741 _05849 Position (kb) 125 126 127 128 129Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 124.419 kb on - strand, within H281DRAFT_05846at 124.690 kb on + strand, within H281DRAFT_05846at 124.694 kb on + strand, within H281DRAFT_05846at 124.694 kb on + strand, within H281DRAFT_05846at 124.694 kb on + strand, within H281DRAFT_05846at 124.694 kb on + strand, within H281DRAFT_05846at 124.695 kb on - strand, within H281DRAFT_05846at 124.695 kb on - strand, within H281DRAFT_05846at 124.695 kb on - strand, within H281DRAFT_05846at 125.018 kb on + strandat 125.019 kb on - strandat 125.019 kb on - strandat 125.184 kb on - strandat 125.184 kb on - strandat 125.666 kb on + strandat 125.666 kb on + strandat 125.667 kb on - strandat 125.667 kb on - strandat 125.667 kb on - strandat 125.667 kb on - strandat 125.730 kb on + strand, within H281DRAFT_05847at 125.731 kb on - strand, within H281DRAFT_05847at 125.792 kb on + strand, within H281DRAFT_05847at 125.810 kb on + strand, within H281DRAFT_05847at 125.810 kb on + strand, within H281DRAFT_05847at 125.811 kb on - strand, within H281DRAFT_05847at 125.811 kb on - strand, within H281DRAFT_05847at 125.823 kb on - strand, within H281DRAFT_05847at 125.945 kb on + strand, within H281DRAFT_05847at 125.946 kb on - strand, within H281DRAFT_05847at 125.972 kb on + strand, within H281DRAFT_05847at 126.015 kb on + strand, within H281DRAFT_05847at 126.015 kb on + strand, within H281DRAFT_05847at 126.016 kb on - strand, within H281DRAFT_05847at 126.081 kb on - strand, within H281DRAFT_05847at 126.081 kb on - strand, within H281DRAFT_05847at 126.171 kb on + strand, within H281DRAFT_05847at 126.192 kb on - strand, within H281DRAFT_05847at 126.192 kb on - strand, within H281DRAFT_05847at 126.192 kb on - strand, within H281DRAFT_05847at 126.192 kb on - strand, within H281DRAFT_05847at 126.297 kb on + strand, within H281DRAFT_05847at 126.298 kb on - strand, within H281DRAFT_05847at 126.702 kb on - strand, within H281DRAFT_05847at 126.887 kb on + strand, within H281DRAFT_05847at 126.888 kb on - strand, within H281DRAFT_05847at 127.046 kb on + strand, within H281DRAFT_05847at 127.046 kb on + strand, within H281DRAFT_05847at 127.046 kb on + strand, within H281DRAFT_05847at 127.047 kb on - strand, within H281DRAFT_05847at 127.154 kb on + strand, within H281DRAFT_05847at 127.155 kb on - strand, within H281DRAFT_05847at 127.284 kb on - strand, within H281DRAFT_05847at 127.284 kb on - strand, within H281DRAFT_05847at 127.284 kb on - strand, within H281DRAFT_05847at 127.295 kb on + strand, within H281DRAFT_05847at 127.295 kb on + strand, within H281DRAFT_05847at 127.296 kb on - strand, within H281DRAFT_05847at 127.296 kb on - strand, within H281DRAFT_05847at 127.299 kb on + strand, within H281DRAFT_05847at 127.299 kb on + strand, within H281DRAFT_05847at 127.299 kb on + strand, within H281DRAFT_05847at 127.299 kb on + strand, within H281DRAFT_05847at 127.299 kb on + strand, within H281DRAFT_05847at 127.299 kb on + strand, within H281DRAFT_05847at 127.300 kb on - strand, within H281DRAFT_05847at 127.300 kb on - strand, within H281DRAFT_05847at 127.300 kb on - strand, within H281DRAFT_05847at 127.355 kb on + strand, within H281DRAFT_05847at 127.356 kb on - strand, within H281DRAFT_05847at 127.484 kb on + strand, within H281DRAFT_05847at 127.485 kb on - strand, within H281DRAFT_05847at 127.485 kb on - strand, within H281DRAFT_05847at 127.485 kb on - strand, within H281DRAFT_05847at 127.720 kb on + strand, within H281DRAFT_05847at 127.720 kb on + strand, within H281DRAFT_05847at 127.721 kb on - strand, within H281DRAFT_05847at 127.838 kb on + strand, within H281DRAFT_05847at 127.838 kb on + strand, within H281DRAFT_05847at 127.838 kb on + strand, within H281DRAFT_05847at 127.838 kb on + strand, within H281DRAFT_05847at 127.838 kb on + strand, within H281DRAFT_05847at 127.838 kb on + strand, within H281DRAFT_05847at 127.839 kb on - strand, within H281DRAFT_05847at 127.839 kb on - strand, within H281DRAFT_05847at 127.964 kb on + strand, within H281DRAFT_05847at 127.964 kb on + strand, within H281DRAFT_05847at 127.965 kb on - strand, within H281DRAFT_05847at 127.965 kb on - strand, within H281DRAFT_05847at 127.965 kb on - strand, within H281DRAFT_05847at 127.965 kb on - strand, within H281DRAFT_05847at 127.972 kb on + strand, within H281DRAFT_05847at 127.972 kb on + strand, within H281DRAFT_05847at 128.039 kb on + strand, within H281DRAFT_05847at 128.040 kb on - strand, within H281DRAFT_05847at 128.040 kb on - strand, within H281DRAFT_05847at 128.040 kb on - strand, within H281DRAFT_05847at 128.121 kb on - strand, within H281DRAFT_05847at 128.136 kb on - strand, within H281DRAFT_05847at 128.363 kb on + strand, within H281DRAFT_05847at 128.364 kb on - strand, within H281DRAFT_05847at 128.586 kb on - strandat 128.601 kb on - strandat 128.767 kb on - strandat 128.840 kb on + strandat 128.840 kb on + strandat 128.841 kb on - strandat 128.841 kb on - strandat 128.864 kb on + strandat 128.864 kb on + strandat 128.864 kb on + strandat 128.864 kb on + strandat 128.864 kb on + strandat 128.865 kb on - strandat 128.865 kb on - strandat 128.890 kb on + strandat 128.891 kb on - strandat 128.926 kb on + strandat 128.941 kb on + strandat 128.941 kb on + strandat 128.941 kb on + strandat 128.947 kb on + strandat 128.947 kb on + strandat 128.947 kb on + strandat 128.947 kb on + strandat 128.948 kb on - strandat 128.948 kb on - strandat 129.007 kb on + strandat 129.025 kb on - strandat 129.025 kb on - strandat 129.038 kb on - strandat 129.047 kb on + strandat 129.047 kb on + strandat 129.047 kb on + strandat 129.047 kb on + strandat 129.048 kb on - strandat 129.048 kb on - strandat 129.048 kb on - strandat 129.149 kb on + strandat 129.149 kb on + strandat 129.150 kb on - strandat 129.202 kb on - strandat 129.202 kb on - strandat 129.219 kb on + strandat 129.219 kb on + strandat 129.219 kb on + strandat 129.219 kb on + strandat 129.219 kb on + strandat 129.220 kb on - strandat 129.220 kb on - strandat 129.220 kb on - strandat 129.283 kb on + strandat 129.283 kb on + strandat 129.284 kb on - strandat 129.284 kb on - strandat 129.297 kb on - strandat 129.302 kb on + strandat 129.494 kb on + strandat 129.504 kb on + strandat 129.505 kb on - strandat 129.550 kb on - strandat 129.569 kb on + strandat 129.570 kb on - strandat 129.593 kb on - strandat 129.897 kb on + strand, within H281DRAFT_05849at 129.897 kb on + strand, within H281DRAFT_05849at 129.897 kb on + strand, within H281DRAFT_05849at 129.897 kb on + strand, within H281DRAFT_05849at 129.898 kb on - strand, within H281DRAFT_05849at 129.900 kb on + strand, within H281DRAFT_05849at 129.900 kb on + strand, within H281DRAFT_05849at 129.900 kb on + strand, within H281DRAFT_05849at 129.901 kb on - strand, within H281DRAFT_05849

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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124,419 - H281DRAFT_05846 0.66 -2.1
124,690 + H281DRAFT_05846 0.78 +0.4
124,694 + H281DRAFT_05846 0.78 +0.1
124,694 + H281DRAFT_05846 0.78 -1.6
124,694 + H281DRAFT_05846 0.78 +0.6
124,694 + H281DRAFT_05846 0.78 +0.0
124,695 - H281DRAFT_05846 0.78 +0.3
124,695 - H281DRAFT_05846 0.78 -2.5
124,695 - H281DRAFT_05846 0.78 +0.5
125,018 + +0.9
125,019 - -0.0
125,019 - +0.3
125,184 - +0.2
125,184 - -1.5
125,666 + +0.6
125,666 + -0.2
125,667 - +0.5
125,667 - +1.0
125,667 - +0.7
125,667 - -0.3
125,730 + H281DRAFT_05847 0.11 +0.5
125,731 - H281DRAFT_05847 0.11 -2.1
125,792 + H281DRAFT_05847 0.13 +2.0
125,810 + H281DRAFT_05847 0.13 +1.0
125,810 + H281DRAFT_05847 0.13 -0.4
125,811 - H281DRAFT_05847 0.13 +0.3
125,811 - H281DRAFT_05847 0.13 +1.0
125,823 - H281DRAFT_05847 0.13 +2.0
125,945 + H281DRAFT_05847 0.17 -0.4
125,946 - H281DRAFT_05847 0.17 -0.2
125,972 + H281DRAFT_05847 0.18 -0.6
126,015 + H281DRAFT_05847 0.19 +0.5
126,015 + H281DRAFT_05847 0.19 +0.1
126,016 - H281DRAFT_05847 0.19 -0.6
126,081 - H281DRAFT_05847 0.21 +0.1
126,081 - H281DRAFT_05847 0.21 -1.3
126,171 + H281DRAFT_05847 0.23 +1.0
126,192 - H281DRAFT_05847 0.24 +0.0
126,192 - H281DRAFT_05847 0.24 -0.5
126,192 - H281DRAFT_05847 0.24 +1.5
126,192 - H281DRAFT_05847 0.24 -0.2
126,297 + H281DRAFT_05847 0.27 -0.1
126,298 - H281DRAFT_05847 0.27 +0.4
126,702 - H281DRAFT_05847 0.38 -0.1
126,887 + H281DRAFT_05847 0.43 -0.1
126,888 - H281DRAFT_05847 0.43 -1.4
127,046 + H281DRAFT_05847 0.47 -0.7
127,046 + H281DRAFT_05847 0.47 +0.2
127,046 + H281DRAFT_05847 0.47 +0.1
127,047 - H281DRAFT_05847 0.47 +0.4
127,154 + H281DRAFT_05847 0.50 -1.5
127,155 - H281DRAFT_05847 0.50 -0.9
127,284 - H281DRAFT_05847 0.54 -1.5
127,284 - H281DRAFT_05847 0.54 -2.3
127,284 - H281DRAFT_05847 0.54 -0.2
127,295 + H281DRAFT_05847 0.54 +0.1
127,295 + H281DRAFT_05847 0.54 -1.5
127,296 - H281DRAFT_05847 0.54 -0.3
127,296 - H281DRAFT_05847 0.54 +0.3
127,299 + H281DRAFT_05847 0.54 +0.5
127,299 + H281DRAFT_05847 0.54 -0.0
127,299 + H281DRAFT_05847 0.54 +0.1
127,299 + H281DRAFT_05847 0.54 +0.8
127,299 + H281DRAFT_05847 0.54 +0.5
127,299 + H281DRAFT_05847 0.54 -2.5
127,300 - H281DRAFT_05847 0.54 -0.5
127,300 - H281DRAFT_05847 0.54 +0.5
127,300 - H281DRAFT_05847 0.54 +0.9
127,355 + H281DRAFT_05847 0.56 -0.1
127,356 - H281DRAFT_05847 0.56 -3.0
127,484 + H281DRAFT_05847 0.60 -1.0
127,485 - H281DRAFT_05847 0.60 -0.8
127,485 - H281DRAFT_05847 0.60 +0.0
127,485 - H281DRAFT_05847 0.60 +1.1
127,720 + H281DRAFT_05847 0.66 -0.4
127,720 + H281DRAFT_05847 0.66 +0.3
127,721 - H281DRAFT_05847 0.66 -2.5
127,838 + H281DRAFT_05847 0.69 -0.1
127,838 + H281DRAFT_05847 0.69 +0.2
127,838 + H281DRAFT_05847 0.69 +0.2
127,838 + H281DRAFT_05847 0.69 -0.1
127,838 + H281DRAFT_05847 0.69 -0.9
127,838 + H281DRAFT_05847 0.69 +0.0
127,839 - H281DRAFT_05847 0.69 -0.3
127,839 - H281DRAFT_05847 0.69 -2.2
127,964 + H281DRAFT_05847 0.73 -0.1
127,964 + H281DRAFT_05847 0.73 -0.2
127,965 - H281DRAFT_05847 0.73 +0.1
127,965 - H281DRAFT_05847 0.73 +0.4
127,965 - H281DRAFT_05847 0.73 -0.9
127,965 - H281DRAFT_05847 0.73 -0.4
127,972 + H281DRAFT_05847 0.73 +0.2
127,972 + H281DRAFT_05847 0.73 +0.1
128,039 + H281DRAFT_05847 0.75 -1.5
128,040 - H281DRAFT_05847 0.75 +0.1
128,040 - H281DRAFT_05847 0.75 -1.2
128,040 - H281DRAFT_05847 0.75 +0.7
128,121 - H281DRAFT_05847 0.77 +1.2
128,136 - H281DRAFT_05847 0.78 +1.1
128,363 + H281DRAFT_05847 0.84 +1.8
128,364 - H281DRAFT_05847 0.84 -0.3
128,586 - -0.7
128,601 - +0.1
128,767 - -0.1
128,840 + +0.2
128,840 + -0.7
128,841 - +0.7
128,841 - -0.8
128,864 + +0.6
128,864 + +1.7
128,864 + -3.5
128,864 + -1.0
128,864 + +0.5
128,865 - -1.2
128,865 - -0.0
128,890 + +0.4
128,891 - +0.6
128,926 + +0.4
128,941 + +0.0
128,941 + +0.5
128,941 + +0.8
128,947 + -0.2
128,947 + +0.5
128,947 + +0.8
128,947 + -0.5
128,948 - +0.0
128,948 - -0.2
129,007 + -1.9
129,025 - -1.8
129,025 - +0.4
129,038 - +0.6
129,047 + -0.5
129,047 + +0.7
129,047 + +0.6
129,047 + -0.8
129,048 - +0.0
129,048 - -0.5
129,048 - +0.2
129,149 + -1.4
129,149 + -0.1
129,150 - -0.3
129,202 - -0.4
129,202 - +0.3
129,219 + -1.5
129,219 + -1.8
129,219 + +0.2
129,219 + -0.0
129,219 + -0.4
129,220 - -1.3
129,220 - -0.4
129,220 - -0.2
129,283 + +0.6
129,283 + -1.0
129,284 - -0.3
129,284 - -0.2
129,297 - -0.5
129,302 + +0.5
129,494 + +0.3
129,504 + -0.1
129,505 - +0.6
129,550 - +0.4
129,569 + +0.1
129,570 - +0.2
129,593 - -0.2
129,897 + H281DRAFT_05849 0.23 -0.1
129,897 + H281DRAFT_05849 0.23 -0.5
129,897 + H281DRAFT_05849 0.23 -0.4
129,897 + H281DRAFT_05849 0.23 +0.3
129,898 - H281DRAFT_05849 0.23 -0.1
129,900 + H281DRAFT_05849 0.23 +0.4
129,900 + H281DRAFT_05849 0.23 -0.4
129,900 + H281DRAFT_05849 0.23 -0.4
129,901 - H281DRAFT_05849 0.23 +0.4

Or see this region's nucleotide sequence