Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05318 and H281DRAFT_05319 are separated by 183 nucleotides H281DRAFT_05319 and H281DRAFT_05320 are separated by 233 nucleotides
H281DRAFT_05318: H281DRAFT_05318 - MFS transporter, ACS family, glucarate transporter, at 68,372 to 69,721
_05318
H281DRAFT_05319: H281DRAFT_05319 - 5-dehydro-4-deoxyglucarate dehydratase, at 69,905 to 70,822
_05319
H281DRAFT_05320: H281DRAFT_05320 - galactarate dehydratase, at 71,056 to 72,645
_05320
Position (kb)
69
70
71 Strain fitness (log2 ratio)
-2
-1
0
1 at 68.911 kb on - strand, within H281DRAFT_05318 at 68.931 kb on + strand, within H281DRAFT_05318 at 69.028 kb on + strand, within H281DRAFT_05318 at 69.029 kb on - strand, within H281DRAFT_05318 at 69.066 kb on + strand, within H281DRAFT_05318 at 69.066 kb on + strand, within H281DRAFT_05318 at 69.067 kb on - strand, within H281DRAFT_05318 at 69.143 kb on + strand, within H281DRAFT_05318 at 69.169 kb on - strand, within H281DRAFT_05318 at 69.221 kb on + strand, within H281DRAFT_05318 at 69.221 kb on + strand, within H281DRAFT_05318 at 69.237 kb on + strand, within H281DRAFT_05318 at 69.517 kb on - strand, within H281DRAFT_05318 at 69.517 kb on - strand, within H281DRAFT_05318 at 69.543 kb on + strand, within H281DRAFT_05318 at 69.544 kb on - strand, within H281DRAFT_05318 at 69.544 kb on - strand, within H281DRAFT_05318 at 69.622 kb on - strand at 69.638 kb on + strand at 69.639 kb on - strand at 69.646 kb on + strand at 69.647 kb on - strand at 69.733 kb on + strand at 69.733 kb on + strand at 69.734 kb on - strand at 69.740 kb on + strand at 69.740 kb on + strand at 69.741 kb on - strand at 69.741 kb on - strand at 69.741 kb on - strand at 70.173 kb on - strand, within H281DRAFT_05319 at 70.209 kb on + strand, within H281DRAFT_05319 at 70.210 kb on - strand, within H281DRAFT_05319 at 70.395 kb on + strand, within H281DRAFT_05319 at 70.395 kb on + strand, within H281DRAFT_05319 at 70.396 kb on - strand, within H281DRAFT_05319 at 70.479 kb on + strand, within H281DRAFT_05319 at 70.479 kb on + strand, within H281DRAFT_05319 at 70.480 kb on - strand, within H281DRAFT_05319 at 70.639 kb on + strand, within H281DRAFT_05319 at 70.640 kb on - strand, within H281DRAFT_05319 at 70.722 kb on + strand, within H281DRAFT_05319 at 70.816 kb on + strand at 70.816 kb on + strand at 70.817 kb on - strand at 70.827 kb on + strand at 70.827 kb on + strand at 70.827 kb on + strand at 70.827 kb on + strand at 70.827 kb on + strand at 70.827 kb on + strand at 70.827 kb on + strand at 70.828 kb on - strand at 70.828 kb on - strand at 70.828 kb on - strand at 70.828 kb on - strand at 70.828 kb on - strand at 70.828 kb on - strand at 70.828 kb on - strand at 70.828 kb on - strand at 70.832 kb on - strand at 70.846 kb on - strand at 70.947 kb on + strand at 70.948 kb on - strand at 70.948 kb on - strand at 70.948 kb on - strand at 70.968 kb on + strand at 70.968 kb on + strand at 70.968 kb on + strand at 70.968 kb on + strand at 70.968 kb on + strand at 70.969 kb on - strand at 71.074 kb on + strand at 71.237 kb on - strand, within H281DRAFT_05320 at 71.238 kb on + strand, within H281DRAFT_05320 at 71.238 kb on + strand, within H281DRAFT_05320 at 71.560 kb on + strand, within H281DRAFT_05320 at 71.560 kb on + strand, within H281DRAFT_05320 at 71.560 kb on + strand, within H281DRAFT_05320 at 71.560 kb on + strand, within H281DRAFT_05320 at 71.561 kb on - strand, within H281DRAFT_05320 at 71.561 kb on - strand, within H281DRAFT_05320 at 71.561 kb on - strand, within H281DRAFT_05320 at 71.623 kb on - strand, within H281DRAFT_05320
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM remove 68,911 - H281DRAFT_05318 0.40 +0.4 68,931 + H281DRAFT_05318 0.41 -1.4 69,028 + H281DRAFT_05318 0.49 -0.3 69,029 - H281DRAFT_05318 0.49 -0.5 69,066 + H281DRAFT_05318 0.51 -0.3 69,066 + H281DRAFT_05318 0.51 +0.4 69,067 - H281DRAFT_05318 0.51 -1.3 69,143 + H281DRAFT_05318 0.57 -0.4 69,169 - H281DRAFT_05318 0.59 -0.2 69,221 + H281DRAFT_05318 0.63 +1.5 69,221 + H281DRAFT_05318 0.63 -0.2 69,237 + H281DRAFT_05318 0.64 +0.9 69,517 - H281DRAFT_05318 0.85 -1.2 69,517 - H281DRAFT_05318 0.85 -0.5 69,543 + H281DRAFT_05318 0.87 -0.2 69,544 - H281DRAFT_05318 0.87 +0.6 69,544 - H281DRAFT_05318 0.87 +0.8 69,622 - -0.3 69,638 + +0.4 69,639 - +0.5 69,646 + +0.7 69,647 - +0.5 69,733 + +0.8 69,733 + -2.1 69,734 - +1.2 69,740 + -0.2 69,740 + +0.0 69,741 - -0.7 69,741 - -0.1 69,741 - +0.4 70,173 - H281DRAFT_05319 0.29 +1.3 70,209 + H281DRAFT_05319 0.33 +0.6 70,210 - H281DRAFT_05319 0.33 +0.5 70,395 + H281DRAFT_05319 0.53 -0.2 70,395 + H281DRAFT_05319 0.53 -0.0 70,396 - H281DRAFT_05319 0.53 +0.3 70,479 + H281DRAFT_05319 0.63 -0.4 70,479 + H281DRAFT_05319 0.63 -0.6 70,480 - H281DRAFT_05319 0.63 +0.5 70,639 + H281DRAFT_05319 0.80 +1.1 70,640 - H281DRAFT_05319 0.80 -0.1 70,722 + H281DRAFT_05319 0.89 +0.3 70,816 + +0.6 70,816 + -0.4 70,817 - +0.6 70,827 + -0.4 70,827 + -1.2 70,827 + -0.1 70,827 + +0.1 70,827 + +0.7 70,827 + -0.2 70,827 + -0.0 70,828 - +0.9 70,828 - -0.1 70,828 - +0.1 70,828 - -1.6 70,828 - +0.6 70,828 - +0.1 70,828 - -0.8 70,828 - -0.8 70,832 - +0.0 70,846 - -0.0 70,947 + +0.6 70,948 - +0.3 70,948 - -0.6 70,948 - -1.0 70,968 + -0.2 70,968 + +0.6 70,968 + +0.4 70,968 + -0.3 70,968 + +0.0 70,969 - -0.8 71,074 + +0.0 71,237 - H281DRAFT_05320 0.11 -0.8 71,238 + H281DRAFT_05320 0.11 +0.8 71,238 + H281DRAFT_05320 0.11 -0.2 71,560 + H281DRAFT_05320 0.32 +0.8 71,560 + H281DRAFT_05320 0.32 +0.8 71,560 + H281DRAFT_05320 0.32 -2.1 71,560 + H281DRAFT_05320 0.32 -0.7 71,561 - H281DRAFT_05320 0.32 +0.4 71,561 - H281DRAFT_05320 0.32 +0.5 71,561 - H281DRAFT_05320 0.32 +0.4 71,623 - H281DRAFT_05320 0.36 +0.2
Or see this region's nucleotide sequence