Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05143

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05141 and H281DRAFT_05142 are separated by 58 nucleotidesH281DRAFT_05142 and H281DRAFT_05143 are separated by 149 nucleotidesH281DRAFT_05143 and H281DRAFT_05144 are separated by 6 nucleotidesH281DRAFT_05144 and H281DRAFT_05145 are separated by 234 nucleotides H281DRAFT_05141: H281DRAFT_05141 - sulfoxide reductase catalytic subunit YedY, at 95,000 to 95,791 _05141 H281DRAFT_05142: H281DRAFT_05142 - hypothetical protein, at 95,850 to 96,464 _05142 H281DRAFT_05143: H281DRAFT_05143 - Predicted membrane protein, at 96,614 to 97,456 _05143 H281DRAFT_05144: H281DRAFT_05144 - amino acid ABC transporter substrate-binding protein, PAAT family, at 97,463 to 98,197 _05144 H281DRAFT_05145: H281DRAFT_05145 - Low affinity Fe/Cu permease, at 98,432 to 98,929 _05145 Position (kb) 96 97 98Strain fitness (log2 ratio) -2 -1 0 1 2 3at 95.651 kb on + strand, within H281DRAFT_05141at 95.652 kb on - strand, within H281DRAFT_05141at 95.739 kb on - strandat 95.769 kb on - strandat 95.846 kb on - strandat 96.187 kb on - strand, within H281DRAFT_05142at 96.187 kb on - strand, within H281DRAFT_05142at 96.279 kb on + strand, within H281DRAFT_05142at 96.280 kb on - strand, within H281DRAFT_05142at 96.319 kb on + strand, within H281DRAFT_05142at 96.320 kb on - strand, within H281DRAFT_05142at 96.323 kb on + strand, within H281DRAFT_05142at 96.323 kb on + strand, within H281DRAFT_05142at 96.323 kb on + strand, within H281DRAFT_05142at 96.323 kb on + strand, within H281DRAFT_05142at 96.411 kb on + strandat 96.411 kb on + strandat 96.412 kb on - strandat 96.463 kb on - strandat 96.779 kb on + strand, within H281DRAFT_05143at 96.939 kb on + strand, within H281DRAFT_05143at 96.993 kb on + strand, within H281DRAFT_05143at 96.993 kb on + strand, within H281DRAFT_05143at 96.994 kb on - strand, within H281DRAFT_05143at 97.219 kb on - strand, within H281DRAFT_05143at 97.273 kb on + strand, within H281DRAFT_05143at 97.273 kb on + strand, within H281DRAFT_05143at 97.274 kb on - strand, within H281DRAFT_05143at 97.274 kb on - strand, within H281DRAFT_05143at 97.275 kb on + strand, within H281DRAFT_05143at 97.275 kb on + strand, within H281DRAFT_05143at 97.446 kb on + strandat 97.446 kb on + strandat 97.447 kb on - strandat 97.884 kb on + strand, within H281DRAFT_05144at 97.905 kb on + strand, within H281DRAFT_05144at 97.906 kb on - strand, within H281DRAFT_05144at 98.317 kb on + strandat 98.433 kb on + strandat 98.433 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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95,651 + H281DRAFT_05141 0.82 -0.3
95,652 - H281DRAFT_05141 0.82 +0.9
95,739 - +0.7
95,769 - +0.5
95,846 - +1.3
96,187 - H281DRAFT_05142 0.55 +0.7
96,187 - H281DRAFT_05142 0.55 -2.3
96,279 + H281DRAFT_05142 0.70 +0.7
96,280 - H281DRAFT_05142 0.70 +1.1
96,319 + H281DRAFT_05142 0.76 +0.5
96,320 - H281DRAFT_05142 0.76 -0.8
96,323 + H281DRAFT_05142 0.77 +0.4
96,323 + H281DRAFT_05142 0.77 +1.2
96,323 + H281DRAFT_05142 0.77 -1.1
96,323 + H281DRAFT_05142 0.77 +0.5
96,411 + -0.2
96,411 + -0.5
96,412 - -0.3
96,463 - -1.7
96,779 + H281DRAFT_05143 0.20 +1.1
96,939 + H281DRAFT_05143 0.39 -0.9
96,993 + H281DRAFT_05143 0.45 +0.1
96,993 + H281DRAFT_05143 0.45 -0.4
96,994 - H281DRAFT_05143 0.45 -0.3
97,219 - H281DRAFT_05143 0.72 -0.3
97,273 + H281DRAFT_05143 0.78 -0.5
97,273 + H281DRAFT_05143 0.78 -0.2
97,274 - H281DRAFT_05143 0.78 -0.3
97,274 - H281DRAFT_05143 0.78 +0.0
97,275 + H281DRAFT_05143 0.78 -0.8
97,275 + H281DRAFT_05143 0.78 +0.8
97,446 + -0.4
97,446 + -0.8
97,447 - +0.0
97,884 + H281DRAFT_05144 0.57 +0.5
97,905 + H281DRAFT_05144 0.60 +0.5
97,906 - H281DRAFT_05144 0.60 -0.1
98,317 + +2.8
98,433 + -0.6
98,433 + +0.6

Or see this region's nucleotide sequence