Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05141

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05139 and H281DRAFT_05140 are separated by 91 nucleotidesH281DRAFT_05140 and H281DRAFT_05141 are separated by 2 nucleotidesH281DRAFT_05141 and H281DRAFT_05142 are separated by 58 nucleotidesH281DRAFT_05142 and H281DRAFT_05143 are separated by 149 nucleotides H281DRAFT_05139: H281DRAFT_05139 - Copper binding protein CusF, at 94,001 to 94,258 _05139 H281DRAFT_05140: H281DRAFT_05140 - Thiosulfate reductase cytochrome b subunit, at 94,350 to 94,997 _05140 H281DRAFT_05141: H281DRAFT_05141 - sulfoxide reductase catalytic subunit YedY, at 95,000 to 95,791 _05141 H281DRAFT_05142: H281DRAFT_05142 - hypothetical protein, at 95,850 to 96,464 _05142 H281DRAFT_05143: H281DRAFT_05143 - Predicted membrane protein, at 96,614 to 97,456 _05143 Position (kb) 94 95 96Strain fitness (log2 ratio) -2 -1 0 1at 94.334 kb on + strandat 94.335 kb on - strandat 94.411 kb on - strandat 94.626 kb on + strand, within H281DRAFT_05140at 94.626 kb on + strand, within H281DRAFT_05140at 94.626 kb on + strand, within H281DRAFT_05140at 94.627 kb on - strand, within H281DRAFT_05140at 94.753 kb on - strand, within H281DRAFT_05140at 94.776 kb on + strandat 94.776 kb on + strand, within H281DRAFT_05140at 95.310 kb on - strand, within H281DRAFT_05141at 95.310 kb on - strand, within H281DRAFT_05141at 95.310 kb on - strand, within H281DRAFT_05141at 95.315 kb on - strand, within H281DRAFT_05141at 95.443 kb on + strand, within H281DRAFT_05141at 95.443 kb on + strand, within H281DRAFT_05141at 95.538 kb on - strand, within H281DRAFT_05141at 95.565 kb on - strand, within H281DRAFT_05141at 95.651 kb on + strand, within H281DRAFT_05141at 95.652 kb on - strand, within H281DRAFT_05141at 95.739 kb on - strandat 95.769 kb on - strandat 95.846 kb on - strandat 96.187 kb on - strand, within H281DRAFT_05142at 96.187 kb on - strand, within H281DRAFT_05142at 96.279 kb on + strand, within H281DRAFT_05142at 96.280 kb on - strand, within H281DRAFT_05142at 96.319 kb on + strand, within H281DRAFT_05142at 96.320 kb on - strand, within H281DRAFT_05142at 96.323 kb on + strand, within H281DRAFT_05142at 96.323 kb on + strand, within H281DRAFT_05142at 96.323 kb on + strand, within H281DRAFT_05142at 96.323 kb on + strand, within H281DRAFT_05142at 96.411 kb on + strandat 96.411 kb on + strandat 96.412 kb on - strandat 96.463 kb on - strandat 96.779 kb on + strand, within H281DRAFT_05143

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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94,334 + -0.2
94,335 - +0.5
94,411 - +0.9
94,626 + H281DRAFT_05140 0.43 -0.6
94,626 + H281DRAFT_05140 0.43 -2.1
94,626 + H281DRAFT_05140 0.43 -0.4
94,627 - H281DRAFT_05140 0.43 -0.4
94,753 - H281DRAFT_05140 0.62 +0.0
94,776 + -0.4
94,776 + H281DRAFT_05140 0.66 -0.3
95,310 - H281DRAFT_05141 0.39 -1.2
95,310 - H281DRAFT_05141 0.39 -0.1
95,310 - H281DRAFT_05141 0.39 +0.5
95,315 - H281DRAFT_05141 0.40 -0.5
95,443 + H281DRAFT_05141 0.56 +0.3
95,443 + H281DRAFT_05141 0.56 -0.0
95,538 - H281DRAFT_05141 0.68 -0.3
95,565 - H281DRAFT_05141 0.71 -1.6
95,651 + H281DRAFT_05141 0.82 -0.3
95,652 - H281DRAFT_05141 0.82 +0.9
95,739 - +0.7
95,769 - +0.5
95,846 - +1.3
96,187 - H281DRAFT_05142 0.55 +0.7
96,187 - H281DRAFT_05142 0.55 -2.3
96,279 + H281DRAFT_05142 0.70 +0.7
96,280 - H281DRAFT_05142 0.70 +1.1
96,319 + H281DRAFT_05142 0.76 +0.5
96,320 - H281DRAFT_05142 0.76 -0.8
96,323 + H281DRAFT_05142 0.77 +0.4
96,323 + H281DRAFT_05142 0.77 +1.2
96,323 + H281DRAFT_05142 0.77 -1.1
96,323 + H281DRAFT_05142 0.77 +0.5
96,411 + -0.2
96,411 + -0.5
96,412 - -0.3
96,463 - -1.7
96,779 + H281DRAFT_05143 0.20 +1.1

Or see this region's nucleotide sequence