Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04481 and H281DRAFT_04482 overlap by 4 nucleotides H281DRAFT_04482 and H281DRAFT_04483 are separated by 167 nucleotides H281DRAFT_04483 and H281DRAFT_04484 are separated by 37 nucleotides H281DRAFT_04484 and H281DRAFT_04485 are separated by 240 nucleotides
H281DRAFT_04481: H281DRAFT_04481 - Uncharacterized membrane protein, at 212,651 to 213,058
_04481
H281DRAFT_04482: H281DRAFT_04482 - NAD(P)H dehydrogenase (quinone), at 213,055 to 213,657
_04482
H281DRAFT_04483: H281DRAFT_04483 - tRNA-processing RNAse BN, at 213,825 to 215,135
_04483
H281DRAFT_04484: H281DRAFT_04484 - hypothetical protein, at 215,173 to 215,493
_04484
H281DRAFT_04485: H281DRAFT_04485 - Pimeloyl-ACP methyl ester carboxylesterase, at 215,734 to 216,567
_04485
Position (kb) 213
214
215
216 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 212.835 kb on + strand at 212.871 kb on + strand, within H281DRAFT_04481 at 212.872 kb on - strand, within H281DRAFT_04481 at 213.227 kb on + strand, within H281DRAFT_04482 at 213.392 kb on + strand at 213.392 kb on + strand, within H281DRAFT_04482 at 213.393 kb on - strand, within H281DRAFT_04482 at 213.632 kb on + strand at 213.633 kb on - strand at 213.633 kb on - strand at 213.635 kb on + strand at 213.635 kb on + strand at 213.636 kb on - strand at 213.658 kb on - strand at 213.678 kb on + strand at 213.679 kb on - strand at 213.681 kb on + strand at 213.681 kb on + strand at 213.681 kb on + strand at 213.682 kb on - strand at 213.683 kb on + strand at 213.778 kb on + strand at 213.903 kb on - strand at 214.081 kb on - strand, within H281DRAFT_04483 at 214.300 kb on - strand, within H281DRAFT_04483 at 214.413 kb on + strand, within H281DRAFT_04483 at 214.413 kb on + strand, within H281DRAFT_04483 at 214.420 kb on - strand, within H281DRAFT_04483 at 214.420 kb on - strand, within H281DRAFT_04483 at 214.512 kb on + strand, within H281DRAFT_04483 at 214.516 kb on - strand, within H281DRAFT_04483 at 214.537 kb on - strand, within H281DRAFT_04483 at 214.590 kb on + strand, within H281DRAFT_04483 at 214.590 kb on + strand, within H281DRAFT_04483 at 214.591 kb on - strand, within H281DRAFT_04483 at 214.591 kb on - strand, within H281DRAFT_04483 at 214.591 kb on - strand, within H281DRAFT_04483 at 214.755 kb on + strand, within H281DRAFT_04483 at 214.923 kb on + strand, within H281DRAFT_04483 at 215.133 kb on + strand at 215.134 kb on - strand at 215.175 kb on - strand at 215.191 kb on + strand at 215.232 kb on + strand, within H281DRAFT_04484 at 215.232 kb on + strand, within H281DRAFT_04484 at 215.235 kb on - strand, within H281DRAFT_04484 at 215.435 kb on + strand, within H281DRAFT_04484 at 215.435 kb on + strand, within H281DRAFT_04484 at 215.435 kb on + strand, within H281DRAFT_04484 at 215.436 kb on - strand, within H281DRAFT_04484 at 215.444 kb on + strand, within H281DRAFT_04484 at 215.484 kb on + strand at 215.838 kb on + strand, within H281DRAFT_04485 at 215.839 kb on - strand, within H281DRAFT_04485 at 215.839 kb on - strand, within H281DRAFT_04485 at 215.839 kb on - strand, within H281DRAFT_04485 at 215.839 kb on - strand, within H281DRAFT_04485 at 215.976 kb on - strand, within H281DRAFT_04485 at 215.979 kb on + strand, within H281DRAFT_04485 at 215.979 kb on + strand, within H281DRAFT_04485 at 215.980 kb on - strand, within H281DRAFT_04485 at 215.980 kb on - strand, within H281DRAFT_04485 at 215.980 kb on - strand, within H281DRAFT_04485
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM remove 212,835 + +0.8 212,871 + H281DRAFT_04481 0.54 +0.7 212,872 - H281DRAFT_04481 0.54 -0.4 213,227 + H281DRAFT_04482 0.29 -1.2 213,392 + +0.8 213,392 + H281DRAFT_04482 0.56 -0.1 213,393 - H281DRAFT_04482 0.56 -1.8 213,632 + -0.8 213,633 - +1.0 213,633 - -0.2 213,635 + -0.2 213,635 + -0.1 213,636 - +0.3 213,658 - +0.4 213,678 + +0.9 213,679 - -0.4 213,681 + +0.2 213,681 + -2.4 213,681 + +0.3 213,682 - -0.3 213,683 + +0.7 213,778 + +0.6 213,903 - +0.3 214,081 - H281DRAFT_04483 0.20 -1.8 214,300 - H281DRAFT_04483 0.36 -0.9 214,413 + H281DRAFT_04483 0.45 +0.5 214,413 + H281DRAFT_04483 0.45 +0.0 214,420 - H281DRAFT_04483 0.45 -0.1 214,420 - H281DRAFT_04483 0.45 +0.4 214,512 + H281DRAFT_04483 0.52 +1.3 214,516 - H281DRAFT_04483 0.53 -0.5 214,537 - H281DRAFT_04483 0.54 +0.5 214,590 + H281DRAFT_04483 0.58 +1.0 214,590 + H281DRAFT_04483 0.58 +0.5 214,591 - H281DRAFT_04483 0.58 +3.1 214,591 - H281DRAFT_04483 0.58 -0.9 214,591 - H281DRAFT_04483 0.58 -1.3 214,755 + H281DRAFT_04483 0.71 +0.4 214,923 + H281DRAFT_04483 0.84 +0.7 215,133 + +0.6 215,134 - -1.2 215,175 - -0.2 215,191 + -0.7 215,232 + H281DRAFT_04484 0.18 -0.1 215,232 + H281DRAFT_04484 0.18 -1.9 215,235 - H281DRAFT_04484 0.19 -0.9 215,435 + H281DRAFT_04484 0.82 +0.4 215,435 + H281DRAFT_04484 0.82 -1.8 215,435 + H281DRAFT_04484 0.82 -0.2 215,436 - H281DRAFT_04484 0.82 +0.1 215,444 + H281DRAFT_04484 0.84 +0.0 215,484 + -0.1 215,838 + H281DRAFT_04485 0.12 -0.2 215,839 - H281DRAFT_04485 0.13 +0.3 215,839 - H281DRAFT_04485 0.13 -2.1 215,839 - H281DRAFT_04485 0.13 -0.5 215,839 - H281DRAFT_04485 0.13 -1.6 215,976 - H281DRAFT_04485 0.29 +0.8 215,979 + H281DRAFT_04485 0.29 +0.3 215,979 + H281DRAFT_04485 0.29 +0.6 215,980 - H281DRAFT_04485 0.29 -0.0 215,980 - H281DRAFT_04485 0.29 -0.7 215,980 - H281DRAFT_04485 0.29 -0.5
Or see this region's nucleotide sequence