Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_03774

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_03773 and H281DRAFT_03774 are separated by 48 nucleotidesH281DRAFT_03774 and H281DRAFT_03775 are separated by 380 nucleotidesH281DRAFT_03775 and H281DRAFT_03776 are separated by 47 nucleotides H281DRAFT_03773: H281DRAFT_03773 - serine/threonine protein kinase, at 289,383 to 291,551 _03773 H281DRAFT_03774: H281DRAFT_03774 - Formylglycine-generating enzyme, required for sulfatase activity, contains SUMF1/FGE domain, at 291,600 to 293,600 _03774 H281DRAFT_03775: H281DRAFT_03775 - protein of unknown function (DUF4399), at 293,981 to 294,484 _03775 H281DRAFT_03776: H281DRAFT_03776 - protein of unknown function (DUF4399), at 294,532 to 294,963 _03776 Position (kb) 291 292 293 294Strain fitness (log2 ratio) -2 -1 0 1 2at 291.071 kb on + strand, within H281DRAFT_03773at 291.222 kb on + strand, within H281DRAFT_03773at 291.266 kb on + strand, within H281DRAFT_03773at 291.590 kb on + strandat 292.045 kb on + strand, within H281DRAFT_03774at 292.251 kb on + strand, within H281DRAFT_03774at 292.619 kb on - strand, within H281DRAFT_03774at 292.656 kb on - strand, within H281DRAFT_03774at 292.690 kb on + strand, within H281DRAFT_03774at 292.767 kb on + strand, within H281DRAFT_03774at 292.768 kb on - strand, within H281DRAFT_03774at 292.997 kb on - strand, within H281DRAFT_03774at 293.082 kb on + strand, within H281DRAFT_03774at 293.298 kb on + strand, within H281DRAFT_03774at 293.433 kb on + strandat 293.661 kb on + strandat 293.694 kb on - strandat 293.879 kb on - strandat 294.053 kb on - strand, within H281DRAFT_03775

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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291,071 + H281DRAFT_03773 0.78 -0.2
291,222 + H281DRAFT_03773 0.85 +2.3
291,266 + H281DRAFT_03773 0.87 -1.2
291,590 + +0.7
292,045 + H281DRAFT_03774 0.22 -0.3
292,251 + H281DRAFT_03774 0.33 +0.6
292,619 - H281DRAFT_03774 0.51 +0.8
292,656 - H281DRAFT_03774 0.53 +0.2
292,690 + H281DRAFT_03774 0.54 +0.7
292,767 + H281DRAFT_03774 0.58 -0.3
292,768 - H281DRAFT_03774 0.58 +0.6
292,997 - H281DRAFT_03774 0.70 -1.7
293,082 + H281DRAFT_03774 0.74 -1.8
293,298 + H281DRAFT_03774 0.85 +1.7
293,433 + -0.1
293,661 + +0.9
293,694 - +0.0
293,879 - -0.0
294,053 - H281DRAFT_03775 0.14 -1.8

Or see this region's nucleotide sequence