Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03422 and H281DRAFT_03423 are separated by 18 nucleotides H281DRAFT_03423 and H281DRAFT_03424 are separated by 88 nucleotides H281DRAFT_03424 and H281DRAFT_03425 are separated by 132 nucleotides
H281DRAFT_03422: H281DRAFT_03422 - dihydroxyacid dehydratase (EC 4.2.1.9), at 495,717 to 497,495
_03422
H281DRAFT_03423: H281DRAFT_03423 - Regulator of RNase E activity RraA, at 497,514 to 498,248
_03423
H281DRAFT_03424: H281DRAFT_03424 - Sugar phosphate permease, at 498,337 to 499,665
_03424
H281DRAFT_03425: H281DRAFT_03425 - transcriptional regulator, LacI family, at 499,798 to 500,865
_03425
Position (kb) 498
499
500 Strain fitness (log2 ratio) -1
0
1 at 498.290 kb on - strand at 498.911 kb on + strand, within H281DRAFT_03424 at 499.099 kb on + strand, within H281DRAFT_03424 at 499.102 kb on + strand, within H281DRAFT_03424 at 499.172 kb on + strand, within H281DRAFT_03424 at 499.173 kb on - strand, within H281DRAFT_03424 at 499.176 kb on + strand, within H281DRAFT_03424 at 499.176 kb on + strand, within H281DRAFT_03424 at 499.177 kb on - strand, within H281DRAFT_03424 at 499.179 kb on + strand, within H281DRAFT_03424 at 499.179 kb on + strand, within H281DRAFT_03424 at 499.390 kb on + strand, within H281DRAFT_03424 at 499.391 kb on - strand, within H281DRAFT_03424
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM remove 498,290 - -1.6 498,911 + H281DRAFT_03424 0.43 +0.4 499,099 + H281DRAFT_03424 0.57 -1.7 499,102 + H281DRAFT_03424 0.58 -0.1 499,172 + H281DRAFT_03424 0.63 +1.2 499,173 - H281DRAFT_03424 0.63 +0.2 499,176 + H281DRAFT_03424 0.63 -0.6 499,176 + H281DRAFT_03424 0.63 +1.7 499,177 - H281DRAFT_03424 0.63 -1.3 499,179 + H281DRAFT_03424 0.63 +1.6 499,179 + H281DRAFT_03424 0.63 -0.9 499,390 + H281DRAFT_03424 0.79 -0.0 499,391 - H281DRAFT_03424 0.79 +0.2
Or see this region's nucleotide sequence