Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03116 and H281DRAFT_03117 are separated by 29 nucleotides H281DRAFT_03117 and H281DRAFT_03118 overlap by 8 nucleotides
H281DRAFT_03116: H281DRAFT_03116 - putative spermidine/putrescine transport system substrate-binding protein, at 149,008 to 150,054
_03116
H281DRAFT_03117: H281DRAFT_03117 - sarcosine oxidase subunit beta, at 150,084 to 151,211
_03117
H281DRAFT_03118: H281DRAFT_03118 - NADPH-dependent 2,4-dienoyl-CoA reductase, sulfur reductase, at 151,204 to 152,637
_03118
Position (kb) 150
151
152 Strain fitness (log2 ratio) -2
-1
0
1
2 at 149.313 kb on - strand, within H281DRAFT_03116 at 149.426 kb on + strand, within H281DRAFT_03116 at 149.427 kb on - strand, within H281DRAFT_03116 at 149.652 kb on - strand, within H281DRAFT_03116 at 149.652 kb on - strand, within H281DRAFT_03116 at 149.652 kb on - strand, within H281DRAFT_03116 at 149.854 kb on - strand, within H281DRAFT_03116 at 150.102 kb on - strand at 150.656 kb on + strand, within H281DRAFT_03117 at 151.056 kb on + strand, within H281DRAFT_03117 at 151.250 kb on + strand at 151.251 kb on - strand at 151.251 kb on - strand at 151.456 kb on + strand, within H281DRAFT_03118 at 152.009 kb on + strand, within H281DRAFT_03118
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM remove 149,313 - H281DRAFT_03116 0.29 +0.1 149,426 + H281DRAFT_03116 0.40 -1.3 149,427 - H281DRAFT_03116 0.40 +0.0 149,652 - H281DRAFT_03116 0.62 +0.4 149,652 - H281DRAFT_03116 0.62 +0.9 149,652 - H281DRAFT_03116 0.62 -1.7 149,854 - H281DRAFT_03116 0.81 -0.7 150,102 - -0.5 150,656 + H281DRAFT_03117 0.51 -0.4 151,056 + H281DRAFT_03117 0.86 -0.6 151,250 + -1.8 151,251 - -0.8 151,251 - -0.7 151,456 + H281DRAFT_03118 0.18 -2.5 152,009 + H281DRAFT_03118 0.56 +1.9
Or see this region's nucleotide sequence