Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_01245

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_01244 and H281DRAFT_01245 are separated by 17 nucleotidesH281DRAFT_01245 and H281DRAFT_01246 are separated by 144 nucleotidesH281DRAFT_01246 and H281DRAFT_01247 are separated by 99 nucleotides H281DRAFT_01244: H281DRAFT_01244 - Formylglycine-generating enzyme, required for sulfatase activity, contains SUMF1/FGE domain, at 80,613 to 81,662 _01244 H281DRAFT_01245: H281DRAFT_01245 - arylsulfatase, at 81,680 to 83,638 _01245 H281DRAFT_01246: H281DRAFT_01246 - hypothetical protein, at 83,783 to 84,214 _01246 H281DRAFT_01247: H281DRAFT_01247 - esterase, PHB depolymerase family, at 84,314 to 85,417 _01247 Position (kb) 81 82 83 84Strain fitness (log2 ratio) -2 -1 0 1at 80.896 kb on + strand, within H281DRAFT_01244at 81.372 kb on - strand, within H281DRAFT_01244at 81.732 kb on + strandat 81.732 kb on + strandat 81.732 kb on + strandat 81.732 kb on + strandat 81.732 kb on + strandat 81.733 kb on - strandat 81.823 kb on - strandat 81.941 kb on - strand, within H281DRAFT_01245at 82.002 kb on + strand, within H281DRAFT_01245at 82.009 kb on - strand, within H281DRAFT_01245at 82.020 kb on + strand, within H281DRAFT_01245at 82.020 kb on + strand, within H281DRAFT_01245at 82.227 kb on + strand, within H281DRAFT_01245at 82.444 kb on - strand, within H281DRAFT_01245at 82.515 kb on + strand, within H281DRAFT_01245at 82.604 kb on - strand, within H281DRAFT_01245at 82.605 kb on + strand, within H281DRAFT_01245at 82.639 kb on - strand, within H281DRAFT_01245at 82.792 kb on - strand, within H281DRAFT_01245at 82.792 kb on - strand, within H281DRAFT_01245at 82.804 kb on - strand, within H281DRAFT_01245at 82.804 kb on - strand, within H281DRAFT_01245at 82.864 kb on - strand, within H281DRAFT_01245at 83.001 kb on + strand, within H281DRAFT_01245at 83.083 kb on - strand, within H281DRAFT_01245at 83.083 kb on - strand, within H281DRAFT_01245at 83.155 kb on + strand, within H281DRAFT_01245at 83.175 kb on + strand, within H281DRAFT_01245at 83.175 kb on + strand, within H281DRAFT_01245at 83.176 kb on - strand, within H281DRAFT_01245at 83.176 kb on - strand, within H281DRAFT_01245at 83.202 kb on - strand, within H281DRAFT_01245at 83.202 kb on - strand, within H281DRAFT_01245at 83.473 kb on - strandat 83.645 kb on - strandat 83.719 kb on + strandat 83.720 kb on - strandat 83.720 kb on - strandat 83.794 kb on + strandat 84.038 kb on - strand, within H281DRAFT_01246at 84.385 kb on + strandat 84.387 kb on + strandat 84.388 kb on - strandat 84.578 kb on + strand, within H281DRAFT_01247at 84.578 kb on + strand, within H281DRAFT_01247at 84.578 kb on + strand, within H281DRAFT_01247at 84.579 kb on - strand, within H281DRAFT_01247

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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80,896 + H281DRAFT_01244 0.27 +0.6
81,372 - H281DRAFT_01244 0.72 +0.1
81,732 + +1.2
81,732 + +1.1
81,732 + -0.1
81,732 + +0.3
81,732 + -0.4
81,733 - +1.1
81,823 - +0.5
81,941 - H281DRAFT_01245 0.13 -1.3
82,002 + H281DRAFT_01245 0.16 +0.4
82,009 - H281DRAFT_01245 0.17 +0.2
82,020 + H281DRAFT_01245 0.17 +0.4
82,020 + H281DRAFT_01245 0.17 -0.2
82,227 + H281DRAFT_01245 0.28 +0.1
82,444 - H281DRAFT_01245 0.39 +0.9
82,515 + H281DRAFT_01245 0.43 +0.4
82,604 - H281DRAFT_01245 0.47 -0.2
82,605 + H281DRAFT_01245 0.47 +0.8
82,639 - H281DRAFT_01245 0.49 -0.8
82,792 - H281DRAFT_01245 0.57 -0.4
82,792 - H281DRAFT_01245 0.57 +0.5
82,804 - H281DRAFT_01245 0.57 -1.6
82,804 - H281DRAFT_01245 0.57 +0.5
82,864 - H281DRAFT_01245 0.60 -0.1
83,001 + H281DRAFT_01245 0.67 +0.7
83,083 - H281DRAFT_01245 0.72 -0.8
83,083 - H281DRAFT_01245 0.72 +0.2
83,155 + H281DRAFT_01245 0.75 +0.4
83,175 + H281DRAFT_01245 0.76 -0.2
83,175 + H281DRAFT_01245 0.76 +0.4
83,176 - H281DRAFT_01245 0.76 +0.2
83,176 - H281DRAFT_01245 0.76 +1.2
83,202 - H281DRAFT_01245 0.78 +0.1
83,202 - H281DRAFT_01245 0.78 -0.7
83,473 - -0.2
83,645 - +1.6
83,719 + -0.0
83,720 - -1.4
83,720 - -0.3
83,794 + -0.1
84,038 - H281DRAFT_01246 0.59 -0.5
84,385 + -1.8
84,387 + +1.2
84,388 - +0.0
84,578 + H281DRAFT_01247 0.24 +0.4
84,578 + H281DRAFT_01247 0.24 -0.1
84,578 + H281DRAFT_01247 0.24 +0.4
84,579 - H281DRAFT_01247 0.24 -1.8

Or see this region's nucleotide sequence