Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_00145

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_00143 and H281DRAFT_00144 overlap by 8 nucleotidesH281DRAFT_00144 and H281DRAFT_00145 overlap by 1 nucleotidesH281DRAFT_00145 and H281DRAFT_00146 are separated by 89 nucleotides H281DRAFT_00143: H281DRAFT_00143 - L-threonine O-3-phosphate decarboxylase, at 148,417 to 149,523 _00143 H281DRAFT_00144: H281DRAFT_00144 - adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 149,516 to 150,451 _00144 H281DRAFT_00145: H281DRAFT_00145 - adenosylcobinamide kinase /adenosylcobinamide-phosphate guanylyltransferase, at 150,451 to 151,005 _00145 H281DRAFT_00146: H281DRAFT_00146 - adenosylcobyric acid synthase (glutamine-hydrolysing), at 151,095 to 152,609 _00146 Position (kb) 150 151 152Strain fitness (log2 ratio) -3 -2 -1 0 1at 149.581 kb on + strandat 149.582 kb on - strandat 149.582 kb on - strandat 149.582 kb on - strandat 149.674 kb on - strand, within H281DRAFT_00144at 149.674 kb on - strand, within H281DRAFT_00144at 150.322 kb on - strand, within H281DRAFT_00144at 150.560 kb on + strand, within H281DRAFT_00145at 150.715 kb on + strand, within H281DRAFT_00145at 150.715 kb on + strand, within H281DRAFT_00145at 150.715 kb on + strand, within H281DRAFT_00145at 150.716 kb on - strand, within H281DRAFT_00145at 150.716 kb on - strand, within H281DRAFT_00145at 150.733 kb on + strand, within H281DRAFT_00145at 150.733 kb on + strand, within H281DRAFT_00145at 150.733 kb on + strand, within H281DRAFT_00145at 150.733 kb on + strand, within H281DRAFT_00145at 150.905 kb on + strand, within H281DRAFT_00145at 151.017 kb on - strandat 151.050 kb on - strandat 151.070 kb on + strandat 151.127 kb on - strandat 151.184 kb on + strandat 151.202 kb on - strandat 151.444 kb on - strand, within H281DRAFT_00146at 151.980 kb on + strand, within H281DRAFT_00146

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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149,581 + -2.5
149,582 - -0.2
149,582 - -0.2
149,582 - -2.1
149,674 - H281DRAFT_00144 0.17 -2.4
149,674 - H281DRAFT_00144 0.17 -0.2
150,322 - H281DRAFT_00144 0.86 -0.4
150,560 + H281DRAFT_00145 0.20 +0.2
150,715 + H281DRAFT_00145 0.48 -0.8
150,715 + H281DRAFT_00145 0.48 -2.0
150,715 + H281DRAFT_00145 0.48 -2.1
150,716 - H281DRAFT_00145 0.48 -3.3
150,716 - H281DRAFT_00145 0.48 -2.4
150,733 + H281DRAFT_00145 0.51 -1.8
150,733 + H281DRAFT_00145 0.51 -2.2
150,733 + H281DRAFT_00145 0.51 -1.5
150,733 + H281DRAFT_00145 0.51 -2.0
150,905 + H281DRAFT_00145 0.82 -2.1
151,017 - +1.4
151,050 - -0.3
151,070 + -2.0
151,127 - +0.9
151,184 + -0.7
151,202 - -3.5
151,444 - H281DRAFT_00146 0.23 -1.1
151,980 + H281DRAFT_00146 0.58 -0.8

Or see this region's nucleotide sequence