Experiment: Bacitracin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2740 and Ac3H11_2741 are separated by 287 nucleotides Ac3H11_2741 and Ac3H11_2742 are separated by 19 nucleotides Ac3H11_2742 and Ac3H11_2743 overlap by 4 nucleotides
Ac3H11_2740 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 1,039,225 to 1,040,109
_2740
Ac3H11_2741 - 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35), at 1,040,397 to 1,041,302
_2741
Ac3H11_2742 - Fumarylacetoacetate hydrolase family protein, at 1,041,322 to 1,042,167
_2742
Ac3H11_2743 - 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase (EC 4.1.1.45), at 1,042,164 to 1,043,252
_2743
Position (kb)
1040
1041
1042 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1040.179 kb on + strand at 1040.200 kb on - strand at 1040.280 kb on + strand at 1040.459 kb on - strand at 1040.511 kb on + strand, within Ac3H11_2741 at 1040.512 kb on - strand, within Ac3H11_2741 at 1041.084 kb on - strand, within Ac3H11_2741 at 1041.294 kb on - strand at 1041.304 kb on + strand at 1041.304 kb on + strand at 1041.304 kb on + strand at 1041.304 kb on + strand at 1041.304 kb on + strand at 1041.304 kb on + strand at 1041.305 kb on - strand at 1041.323 kb on + strand at 1041.731 kb on + strand, within Ac3H11_2742 at 1041.936 kb on - strand, within Ac3H11_2742 at 1041.936 kb on - strand, within Ac3H11_2742
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.016 mg/ml remove 1,040,179 + -3.0 1,040,200 - +0.1 1,040,280 + +1.1 1,040,459 - +1.5 1,040,511 + Ac3H11_2741 0.13 -0.9 1,040,512 - Ac3H11_2741 0.13 -0.4 1,041,084 - Ac3H11_2741 0.76 -0.7 1,041,294 - -0.2 1,041,304 + +0.2 1,041,304 + -0.0 1,041,304 + +0.2 1,041,304 + +0.2 1,041,304 + +0.2 1,041,304 + -0.2 1,041,305 - -1.5 1,041,323 + -0.5 1,041,731 + Ac3H11_2742 0.48 +2.5 1,041,936 - Ac3H11_2742 0.73 -0.5 1,041,936 - Ac3H11_2742 0.73 -0.4
Or see this region's nucleotide sequence