Experiment: Bacitracin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2139 and Ac3H11_2140 are separated by 150 nucleotides Ac3H11_2140 and Ac3H11_2141 are separated by 169 nucleotides Ac3H11_2141 and Ac3H11_2142 are separated by 316 nucleotides
Ac3H11_2139 - Translation elongation factor G, at 369,138 to 371,240
_2139
Ac3H11_2140 - SSU ribosomal protein S7p (S5e), at 371,391 to 371,864
_2140
Ac3H11_2141 - SSU ribosomal protein S12p (S23e), at 372,034 to 372,411
_2141
Ac3H11_2142 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 372,728 to 373,897
_2142
Position (kb) 371
372 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 372.511 kb on - strand at 372.511 kb on - strand at 372.607 kb on - strand at 372.729 kb on + strand at 372.729 kb on + strand at 372.729 kb on + strand at 372.729 kb on + strand at 372.729 kb on + strand at 372.729 kb on + strand at 372.730 kb on - strand at 372.730 kb on - strand at 372.730 kb on - strand at 372.730 kb on - strand at 372.730 kb on - strand at 372.730 kb on - strand at 372.730 kb on - strand at 372.730 kb on - strand at 372.734 kb on - strand at 372.734 kb on - strand at 372.734 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.016 mg/ml remove 372,511 - -0.1 372,511 - -3.1 372,607 - -0.3 372,729 + -1.4 372,729 + -2.2 372,729 + -0.2 372,729 + -0.3 372,729 + -0.3 372,729 + -0.0 372,730 - -0.2 372,730 - -0.8 372,730 - +0.1 372,730 - +0.7 372,730 - +0.8 372,730 - -0.8 372,730 - -2.2 372,730 - +0.1 372,734 - -0.7 372,734 - +0.5 372,734 - -0.9
Or see this region's nucleotide sequence