Experiment: Bacitracin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1817 and Ac3H11_1818 are separated by 48 nucleotides Ac3H11_1818 and Ac3H11_1819 are separated by 29 nucleotides Ac3H11_1819 and Ac3H11_1820 are separated by 15 nucleotides
Ac3H11_1817 - tRNA(Cytosine32)-2-thiocytidine synthetase, at 22,284 to 23,225
_1817
Ac3H11_1818 - Dihydroneopterin aldolase (EC 4.1.2.25), at 23,274 to 23,648
_1818
Ac3H11_1819 - FolM Alternative dihydrofolate reductase 1, at 23,678 to 24,463
_1819
Ac3H11_1820 - COG1565: Uncharacterized conserved protein, at 24,479 to 25,597
_1820
Position (kb)
23
24
25 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 22.787 kb on - strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.864 kb on + strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.865 kb on - strand, within Ac3H11_1817 at 22.912 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.029 kb on + strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.030 kb on - strand, within Ac3H11_1817 at 23.219 kb on + strand at 23.219 kb on + strand at 23.220 kb on - strand at 23.973 kb on + strand, within Ac3H11_1819 at 23.974 kb on - strand, within Ac3H11_1819 at 24.318 kb on - strand, within Ac3H11_1819 at 24.318 kb on - strand, within Ac3H11_1819 at 24.457 kb on + strand at 24.457 kb on + strand at 24.458 kb on - strand at 24.458 kb on - strand at 24.458 kb on - strand at 24.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.016 mg/ml remove 22,787 - Ac3H11_1817 0.53 +0.5 22,864 + Ac3H11_1817 0.62 +1.3 22,864 + Ac3H11_1817 0.62 +0.2 22,864 + Ac3H11_1817 0.62 +0.2 22,864 + Ac3H11_1817 0.62 -0.3 22,864 + Ac3H11_1817 0.62 -0.0 22,864 + Ac3H11_1817 0.62 +0.4 22,864 + Ac3H11_1817 0.62 -0.0 22,864 + Ac3H11_1817 0.62 +0.2 22,864 + Ac3H11_1817 0.62 +0.4 22,864 + Ac3H11_1817 0.62 -1.5 22,864 + Ac3H11_1817 0.62 -0.4 22,864 + Ac3H11_1817 0.62 +1.0 22,864 + Ac3H11_1817 0.62 +0.6 22,865 - Ac3H11_1817 0.62 +1.2 22,865 - Ac3H11_1817 0.62 -2.7 22,865 - Ac3H11_1817 0.62 +1.0 22,865 - Ac3H11_1817 0.62 -1.8 22,865 - Ac3H11_1817 0.62 +0.6 22,865 - Ac3H11_1817 0.62 +0.5 22,865 - Ac3H11_1817 0.62 -0.8 22,865 - Ac3H11_1817 0.62 +0.1 22,865 - Ac3H11_1817 0.62 +1.7 22,865 - Ac3H11_1817 0.62 +1.6 22,912 + Ac3H11_1817 0.67 -1.2 23,029 + Ac3H11_1817 0.79 -0.7 23,029 + Ac3H11_1817 0.79 +1.4 23,029 + Ac3H11_1817 0.79 -0.1 23,029 + Ac3H11_1817 0.79 +0.2 23,029 + Ac3H11_1817 0.79 -0.1 23,029 + Ac3H11_1817 0.79 -0.0 23,029 + Ac3H11_1817 0.79 +2.7 23,029 + Ac3H11_1817 0.79 +0.8 23,029 + Ac3H11_1817 0.79 +0.6 23,029 + Ac3H11_1817 0.79 +0.3 23,029 + Ac3H11_1817 0.79 +0.7 23,030 - Ac3H11_1817 0.79 -1.3 23,030 - Ac3H11_1817 0.79 -0.2 23,030 - Ac3H11_1817 0.79 +1.3 23,030 - Ac3H11_1817 0.79 +0.5 23,030 - Ac3H11_1817 0.79 +1.8 23,030 - Ac3H11_1817 0.79 +1.4 23,030 - Ac3H11_1817 0.79 -0.1 23,219 + +0.5 23,219 + +1.2 23,220 - +0.2 23,973 + Ac3H11_1819 0.38 -0.8 23,974 - Ac3H11_1819 0.38 -3.5 24,318 - Ac3H11_1819 0.81 -2.4 24,318 - Ac3H11_1819 0.81 -2.6 24,457 + -2.8 24,457 + -1.9 24,458 - -0.9 24,458 - -0.6 24,458 - -0.2 24,458 - +0.5
Or see this region's nucleotide sequence