Experiment: amylopectin from maize
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1237 and BT1238 are separated by 75 nucleotides BT1238 and BT1239 overlap by 29 nucleotides BT1239 and BT1240 are separated by 103 nucleotides
BT1237: BT1237 - two-component system sensor histidine kinase (NCBI ptt file), at 1,538,986 to 1,540,911
BT1237
BT1238: BT1238 - putative RNA binding protein with S1 RNA-binding domain (NCBI ptt file), at 1,540,987 to 1,543,161
BT1238
BT1239: BT1239 - putative metal-dependent membrane protease (NCBI ptt file), at 1,543,133 to 1,544,029
BT1239
BT1240: BT1240 - conserved hypothetical protein (NCBI ptt file), at 1,544,133 to 1,545,182
BT1240
Position (kb)
1540
1541
1542
1543
1544 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1540.115 kb on + strand, within BT1237 at 1540.119 kb on + strand, within BT1237 at 1540.119 kb on + strand, within BT1237 at 1540.219 kb on - strand, within BT1237 at 1540.227 kb on + strand, within BT1237 at 1540.317 kb on - strand, within BT1237 at 1540.397 kb on + strand, within BT1237 at 1540.581 kb on - strand, within BT1237 at 1540.722 kb on + strand at 1540.749 kb on - strand at 1540.760 kb on + strand at 1540.812 kb on + strand at 1540.850 kb on - strand at 1540.884 kb on + strand at 1540.898 kb on - strand at 1540.899 kb on + strand at 1540.901 kb on + strand at 1540.901 kb on + strand at 1540.902 kb on - strand at 1540.902 kb on - strand at 1540.912 kb on - strand at 1540.956 kb on + strand at 1540.959 kb on - strand at 1540.987 kb on - strand at 1541.060 kb on + strand at 1541.332 kb on + strand, within BT1238 at 1541.639 kb on - strand, within BT1238 at 1541.780 kb on + strand, within BT1238 at 1541.810 kb on + strand, within BT1238 at 1542.054 kb on + strand, within BT1238 at 1542.055 kb on - strand, within BT1238 at 1542.058 kb on + strand, within BT1238 at 1542.059 kb on - strand, within BT1238 at 1542.124 kb on + strand, within BT1238 at 1542.505 kb on + strand, within BT1238 at 1542.506 kb on - strand, within BT1238 at 1542.506 kb on - strand, within BT1238 at 1542.700 kb on + strand, within BT1238 at 1542.701 kb on - strand, within BT1238 at 1542.992 kb on + strand at 1542.992 kb on + strand at 1542.993 kb on - strand at 1543.016 kb on + strand at 1543.016 kb on + strand at 1543.042 kb on + strand at 1543.043 kb on - strand at 1543.208 kb on - strand at 1543.259 kb on + strand, within BT1239 at 1543.559 kb on + strand, within BT1239 at 1543.927 kb on - strand, within BT1239 at 1544.034 kb on - strand at 1544.067 kb on - strand at 1544.067 kb on - strand at 1544.104 kb on + strand at 1544.149 kb on + strand at 1544.150 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction amylopectin from maize remove 1,540,115 + BT1237 0.59 -1.5 1,540,119 + BT1237 0.59 -2.9 1,540,119 + BT1237 0.59 -0.2 1,540,219 - BT1237 0.64 -1.4 1,540,227 + BT1237 0.64 -1.2 1,540,317 - BT1237 0.69 -1.7 1,540,397 + BT1237 0.73 -0.6 1,540,581 - BT1237 0.83 -0.9 1,540,722 + +1.8 1,540,749 - -2.9 1,540,760 + -1.2 1,540,812 + -0.8 1,540,850 - -1.4 1,540,884 + -1.0 1,540,898 - +0.2 1,540,899 + +0.2 1,540,901 + -1.2 1,540,901 + +0.9 1,540,902 - -2.9 1,540,902 - -0.6 1,540,912 - +0.3 1,540,956 + -1.1 1,540,959 - +1.5 1,540,987 - -0.7 1,541,060 + +0.8 1,541,332 + BT1238 0.16 -1.6 1,541,639 - BT1238 0.30 +0.4 1,541,780 + BT1238 0.36 -0.2 1,541,810 + BT1238 0.38 -2.2 1,542,054 + BT1238 0.49 +0.2 1,542,055 - BT1238 0.49 +2.3 1,542,058 + BT1238 0.49 -0.5 1,542,059 - BT1238 0.49 +0.9 1,542,124 + BT1238 0.52 -0.2 1,542,505 + BT1238 0.70 -1.2 1,542,506 - BT1238 0.70 -1.8 1,542,506 - BT1238 0.70 +1.8 1,542,700 + BT1238 0.79 +2.2 1,542,701 - BT1238 0.79 -0.6 1,542,992 + -1.4 1,542,992 + +0.8 1,542,993 - +0.8 1,543,016 + -1.8 1,543,016 + +0.1 1,543,042 + -2.6 1,543,043 - +2.3 1,543,208 - -1.7 1,543,259 + BT1239 0.14 -0.2 1,543,559 + BT1239 0.47 -0.5 1,543,927 - BT1239 0.89 +0.8 1,544,034 - +0.8 1,544,067 - -1.9 1,544,067 - +0.8 1,544,104 + -1.6 1,544,149 + -1.4 1,544,150 - +2.3
Or see this region's nucleotide sequence