Experiment: amylopectin from maize
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0025 and BT0026 are separated by 261 nucleotides BT0026 and BT0027 are separated by 185 nucleotides BT0027 and BT0028 are separated by 471 nucleotides BT0028 and BT0029 are separated by 75 nucleotides
BT0025: BT0025 - putative transposase (NCBI ptt file), at 20,537 to 20,674
BT0025
BT0026: BT0026 - putative transposase (NCBI ptt file), at 20,936 to 21,358
BT0026
BT0027: BT0027 - putative transposase (NCBI ptt file), at 21,544 to 22,080
BT0027
BT0028: BT0028 - alkaline phosphatase (NCBI ptt file), at 22,552 to 22,830
BT0028
BT0029: BT0029 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 22,906 to 26,169
BT0029
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 20.621 kb on - strand, within BT0025 at 20.624 kb on - strand, within BT0025 at 20.699 kb on + strand at 20.701 kb on + strand at 20.701 kb on + strand at 20.740 kb on - strand at 20.835 kb on - strand at 20.998 kb on - strand, within BT0026 at 21.454 kb on - strand at 21.467 kb on - strand at 21.496 kb on + strand at 21.583 kb on - strand at 21.657 kb on + strand, within BT0027 at 21.657 kb on + strand, within BT0027 at 21.658 kb on - strand, within BT0027 at 21.658 kb on - strand, within BT0027 at 21.658 kb on - strand, within BT0027 at 21.658 kb on - strand, within BT0027 at 21.679 kb on - strand, within BT0027 at 21.715 kb on - strand, within BT0027 at 21.719 kb on - strand, within BT0027 at 21.727 kb on + strand, within BT0027 at 21.728 kb on - strand, within BT0027 at 21.802 kb on - strand, within BT0027 at 21.803 kb on + strand, within BT0027 at 21.804 kb on - strand, within BT0027 at 21.957 kb on + strand, within BT0027 at 21.969 kb on + strand, within BT0027 at 21.969 kb on + strand, within BT0027 at 22.102 kb on + strand at 22.115 kb on + strand at 22.119 kb on - strand at 22.258 kb on - strand at 22.259 kb on + strand at 22.336 kb on - strand at 22.440 kb on - strand at 22.614 kb on + strand, within BT0028 at 22.634 kb on + strand, within BT0028 at 22.635 kb on - strand, within BT0028 at 22.635 kb on - strand, within BT0028 at 22.639 kb on - strand, within BT0028 at 22.650 kb on + strand, within BT0028 at 22.685 kb on - strand, within BT0028 at 22.690 kb on - strand, within BT0028 at 22.841 kb on - strand at 22.861 kb on - strand at 22.900 kb on + strand at 23.039 kb on + strand at 23.060 kb on + strand at 23.078 kb on + strand at 23.079 kb on - strand at 23.079 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction amylopectin from maize remove 20,621 - BT0025 0.61 +0.4 20,624 - BT0025 0.63 -1.4 20,699 + -2.8 20,701 + +1.6 20,701 + -2.3 20,740 - +0.0 20,835 - -2.0 20,998 - BT0026 0.15 -1.2 21,454 - -1.6 21,467 - +0.3 21,496 + -0.8 21,583 - +0.6 21,657 + BT0027 0.21 -1.8 21,657 + BT0027 0.21 -1.0 21,658 - BT0027 0.21 -1.2 21,658 - BT0027 0.21 +0.6 21,658 - BT0027 0.21 -1.6 21,658 - BT0027 0.21 -0.4 21,679 - BT0027 0.25 -2.0 21,715 - BT0027 0.32 -1.0 21,719 - BT0027 0.33 -3.0 21,727 + BT0027 0.34 +0.6 21,728 - BT0027 0.34 +0.0 21,802 - BT0027 0.48 +0.0 21,803 + BT0027 0.48 -1.0 21,804 - BT0027 0.48 -1.2 21,957 + BT0027 0.77 -2.2 21,969 + BT0027 0.79 -1.2 21,969 + BT0027 0.79 -1.0 22,102 + -3.8 22,115 + -3.0 22,119 - -0.9 22,258 - -0.4 22,259 + -0.4 22,336 - +1.6 22,440 - -1.4 22,614 + BT0028 0.22 -0.4 22,634 + BT0028 0.29 -1.6 22,635 - BT0028 0.30 -0.7 22,635 - BT0028 0.30 +0.3 22,639 - BT0028 0.31 -1.5 22,650 + BT0028 0.35 +1.2 22,685 - BT0028 0.48 -3.1 22,690 - BT0028 0.49 +0.6 22,841 - -0.4 22,861 - -0.3 22,900 + -1.3 23,039 + -0.4 23,060 + -3.3 23,078 + +1.6 23,079 - +0.3 23,079 - -1.8
Or see this region's nucleotide sequence