Strain Fitness in Methanococcus maripaludis S2 around MMP_RS07740

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS07735 and MMP_RS07740 are separated by 21 nucleotidesMMP_RS07740 and MMP_RS07745 are separated by 14 nucleotidesMMP_RS07745 and MMP_RS07750 are separated by 267 nucleotides MMP_RS07735: MMP_RS07735 - 2-ketoisovalerate ferredoxin oxidoreductase subunit alpha, at 1,464,410 to 1,465,573 _RS07735 MMP_RS07740: MMP_RS07740 - 4Fe-4S binding protein, at 1,465,595 to 1,465,852 _RS07740 MMP_RS07745: MMP_RS07745 - pyruvate ferredoxin oxidoreductase subunit gamma, at 1,465,867 to 1,466,397 _RS07745 MMP_RS07750: MMP_RS07750 - DUF357 domain-containing protein, at 1,466,665 to 1,466,970 _RS07750 Position (kb) 1465 1466Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1464.773 kb on - strand, within MMP_RS07735at 1464.993 kb on - strand, within MMP_RS07735at 1465.827 kb on - strandat 1466.148 kb on - strandat 1466.189 kb on + strandat 1466.474 kb on + strandat 1466.534 kb on + strandat 1466.574 kb on + strandat 1466.583 kb on + strandat 1466.583 kb on + strandat 1466.588 kb on - strandat 1466.682 kb on - strandat 1466.683 kb on + strandat 1466.843 kb on - strand, within MMP_RS07750

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
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1,464,773 - MMP_RS07735 0.31 -2.3
1,464,993 - MMP_RS07735 0.50 -0.8
1,465,827 - -1.4
1,466,148 - -1.4
1,466,189 + +0.4
1,466,474 + -0.5
1,466,534 + +0.7
1,466,574 + +0.2
1,466,583 + -1.2
1,466,583 + +0.4
1,466,588 - +3.2
1,466,682 - -0.2
1,466,683 + -1.1
1,466,843 - MMP_RS07750 0.58 -2.5

Or see this region's nucleotide sequence