Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07605 and MMP_RS07610 are separated by 113 nucleotides MMP_RS07610 and MMP_RS07615 are separated by 172 nucleotides MMP_RS07615 and MMP_RS07620 overlap by 1 nucleotides
MMP_RS07605: MMP_RS07605 - diadenylate cyclase, at 1,439,730 to 1,440,662
_RS07605
MMP_RS07610: MMP_RS07610 - homoaconitase large subunit, at 1,440,776 to 1,442,032
_RS07610
MMP_RS07615: MMP_RS07615 - energy-coupling factor ABC transporter permease, at 1,442,205 to 1,442,882
_RS07615
MMP_RS07620: MMP_RS07620 - energy-coupling factor ABC transporter substrate-binding protein, at 1,442,882 to 1,443,178
_RS07620
Position (kb)
1440
1441
1442
1443 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1439.987 kb on - strand, within MMP_RS07605 at 1439.988 kb on + strand, within MMP_RS07605 at 1440.123 kb on + strand, within MMP_RS07605 at 1440.160 kb on + strand, within MMP_RS07605 at 1440.692 kb on - strand at 1440.693 kb on + strand at 1440.704 kb on + strand at 1440.710 kb on + strand at 1440.725 kb on + strand at 1440.729 kb on - strand at 1440.730 kb on + strand at 1440.749 kb on - strand at 1440.749 kb on - strand at 1440.749 kb on - strand at 1440.749 kb on - strand at 1440.750 kb on + strand at 1440.750 kb on + strand at 1440.750 kb on + strand at 1440.750 kb on + strand at 1441.005 kb on - strand at 1441.335 kb on + strand at 1441.456 kb on - strand at 1441.456 kb on - strand at 1441.462 kb on - strand at 1441.467 kb on + strand at 1441.789 kb on - strand at 1441.813 kb on + strand at 1441.845 kb on - strand at 1441.850 kb on + strand at 1441.908 kb on - strand at 1441.909 kb on + strand at 1442.021 kb on + strand at 1442.070 kb on + strand at 1442.073 kb on - strand at 1442.074 kb on + strand at 1442.074 kb on + strand at 1442.074 kb on + strand at 1442.076 kb on - strand at 1442.077 kb on + strand at 1442.079 kb on - strand at 1442.097 kb on - strand at 1442.099 kb on - strand at 1442.099 kb on - strand at 1442.100 kb on + strand at 1442.100 kb on + strand at 1442.109 kb on + strand at 1442.143 kb on - strand at 1442.143 kb on - strand at 1442.143 kb on - strand at 1442.143 kb on - strand at 1442.144 kb on + strand at 1442.145 kb on - strand at 1442.146 kb on + strand at 1442.180 kb on - strand at 1442.181 kb on + strand at 1442.195 kb on + strand at 1442.277 kb on + strand, within MMP_RS07615 at 1442.278 kb on - strand, within MMP_RS07615 at 1442.279 kb on + strand, within MMP_RS07615 at 1442.279 kb on + strand, within MMP_RS07615 at 1442.500 kb on - strand, within MMP_RS07615 at 1442.609 kb on - strand, within MMP_RS07615 at 1442.769 kb on + strand, within MMP_RS07615 at 1442.779 kb on - strand, within MMP_RS07615 at 1442.793 kb on + strand, within MMP_RS07615 at 1442.816 kb on + strand at 1442.881 kb on + strand at 1442.926 kb on - strand, within MMP_RS07620 at 1442.948 kb on - strand, within MMP_RS07620 at 1442.975 kb on - strand, within MMP_RS07620
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 1,439,987 - MMP_RS07605 0.28 -3.0 1,439,988 + MMP_RS07605 0.28 -2.5 1,440,123 + MMP_RS07605 0.42 -3.3 1,440,160 + MMP_RS07605 0.46 -5.0 1,440,692 - -0.5 1,440,693 + +0.6 1,440,704 + +0.5 1,440,710 + -0.3 1,440,725 + -0.3 1,440,729 - -3.1 1,440,730 + -0.1 1,440,749 - -4.2 1,440,749 - -2.4 1,440,749 - -2.5 1,440,749 - -4.9 1,440,750 + -1.5 1,440,750 + -0.1 1,440,750 + -2.6 1,440,750 + -0.2 1,441,005 - -3.4 1,441,335 + -4.4 1,441,456 - -2.9 1,441,456 - -1.5 1,441,462 - -2.2 1,441,467 + -2.5 1,441,789 - -2.6 1,441,813 + -3.1 1,441,845 - -2.9 1,441,850 + -2.1 1,441,908 - -2.2 1,441,909 + -2.3 1,442,021 + -2.8 1,442,070 + -0.2 1,442,073 - +0.7 1,442,074 + +3.5 1,442,074 + -0.6 1,442,074 + -0.3 1,442,076 - -2.4 1,442,077 + +0.7 1,442,079 - +0.0 1,442,097 - +0.0 1,442,099 - +0.0 1,442,099 - -0.2 1,442,100 + +0.0 1,442,100 + +0.8 1,442,109 + -0.4 1,442,143 - +0.7 1,442,143 - -0.7 1,442,143 - -2.8 1,442,143 - -1.0 1,442,144 + -0.0 1,442,145 - -0.4 1,442,146 + +1.0 1,442,180 - -0.0 1,442,181 + +0.5 1,442,195 + -0.5 1,442,277 + MMP_RS07615 0.11 -0.4 1,442,278 - MMP_RS07615 0.11 -0.1 1,442,279 + MMP_RS07615 0.11 +0.3 1,442,279 + MMP_RS07615 0.11 +0.3 1,442,500 - MMP_RS07615 0.44 +2.6 1,442,609 - MMP_RS07615 0.60 +0.1 1,442,769 + MMP_RS07615 0.83 +0.1 1,442,779 - MMP_RS07615 0.85 +0.6 1,442,793 + MMP_RS07615 0.87 -0.5 1,442,816 + +0.3 1,442,881 + -0.4 1,442,926 - MMP_RS07620 0.15 +0.4 1,442,948 - MMP_RS07620 0.22 -0.3 1,442,975 - MMP_RS07620 0.31 -1.7
Or see this region's nucleotide sequence