Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03215 and MMP_RS03220 are separated by 1 nucleotides MMP_RS03220 and MMP_RS08955 are separated by 8 nucleotides MMP_RS08955 and MMP_RS03225 are separated by 80 nucleotides MMP_RS03225 and MMP_RS03230 are separated by 95 nucleotides MMP_RS03230 and MMP_RS03235 are separated by 213 nucleotides
MMP_RS03215: MMP_RS03215 - MBL fold metallo-hydrolase, at 606,974 to 607,516
_RS03215
MMP_RS03220: MMP_RS03220 - RNA 2' 3'-cyclic phosphodiesterase, at 607,518 to 608,042
_RS03220
MMP_RS08955: MMP_RS08955 - hypothetical protein, at 608,051 to 608,197
_RS08955
MMP_RS03225: MMP_RS03225 - acetolactate decarboxylase, at 608,278 to 609,078
_RS03225
MMP_RS03230: MMP_RS03230 - flavodoxin family protein, at 609,174 to 609,749
_RS03230
MMP_RS03235: MMP_RS03235 - tRNA pseudouridine(13) synthase TruD, at 609,963 to 611,255
_RS03235
Position (kb) 608
609
610 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 607.330 kb on + strand, within MMP_RS03215 at 607.343 kb on - strand, within MMP_RS03215 at 607.405 kb on - strand, within MMP_RS03215 at 607.414 kb on + strand, within MMP_RS03215 at 607.414 kb on + strand, within MMP_RS03215 at 607.453 kb on + strand, within MMP_RS03215 at 607.474 kb on + strand at 607.474 kb on + strand at 607.520 kb on + strand at 607.559 kb on - strand at 607.560 kb on + strand at 607.560 kb on + strand at 607.569 kb on + strand at 607.927 kb on + strand, within MMP_RS03220 at 607.927 kb on + strand, within MMP_RS03220 at 608.230 kb on - strand at 608.245 kb on + strand at 608.262 kb on + strand at 608.331 kb on - strand at 608.462 kb on - strand, within MMP_RS03225 at 608.483 kb on - strand, within MMP_RS03225 at 608.536 kb on - strand, within MMP_RS03225 at 608.536 kb on - strand, within MMP_RS03225 at 608.808 kb on + strand, within MMP_RS03225 at 609.119 kb on + strand at 609.138 kb on - strand at 609.181 kb on - strand at 609.182 kb on + strand at 609.230 kb on + strand at 609.237 kb on + strand, within MMP_RS03230 at 609.283 kb on - strand, within MMP_RS03230 at 609.283 kb on - strand, within MMP_RS03230 at 609.284 kb on + strand, within MMP_RS03230 at 609.315 kb on + strand, within MMP_RS03230 at 609.324 kb on - strand, within MMP_RS03230 at 609.541 kb on - strand, within MMP_RS03230 at 609.548 kb on - strand, within MMP_RS03230 at 609.828 kb on - strand at 609.932 kb on + strand at 609.932 kb on + strand at 609.951 kb on - strand at 609.952 kb on + strand at 609.952 kb on + strand at 609.952 kb on + strand at 609.954 kb on + strand at 609.955 kb on - strand at 609.955 kb on - strand at 609.956 kb on + strand at 609.956 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 607,330 + MMP_RS03215 0.66 +1.9 607,343 - MMP_RS03215 0.68 +2.4 607,405 - MMP_RS03215 0.79 +0.9 607,414 + MMP_RS03215 0.81 +1.8 607,414 + MMP_RS03215 0.81 +1.4 607,453 + MMP_RS03215 0.88 +2.0 607,474 + +1.0 607,474 + +2.5 607,520 + +0.6 607,559 - -0.4 607,560 + -0.1 607,560 + +0.5 607,569 + +0.5 607,927 + MMP_RS03220 0.78 -0.0 607,927 + MMP_RS03220 0.78 +0.0 608,230 - +0.0 608,245 + +0.4 608,262 + -0.2 608,331 - +0.0 608,462 - MMP_RS03225 0.23 -0.0 608,483 - MMP_RS03225 0.26 -0.0 608,536 - MMP_RS03225 0.32 -0.3 608,536 - MMP_RS03225 0.32 -0.4 608,808 + MMP_RS03225 0.66 +0.0 609,119 + -0.6 609,138 - +0.2 609,181 - +0.3 609,182 + +0.8 609,230 + +0.7 609,237 + MMP_RS03230 0.11 +0.2 609,283 - MMP_RS03230 0.19 -0.6 609,283 - MMP_RS03230 0.19 +3.3 609,284 + MMP_RS03230 0.19 +1.0 609,315 + MMP_RS03230 0.24 -0.4 609,324 - MMP_RS03230 0.26 -0.0 609,541 - MMP_RS03230 0.64 +0.5 609,548 - MMP_RS03230 0.65 -0.1 609,828 - +0.1 609,932 + -2.3 609,932 + +0.2 609,951 - -1.7 609,952 + -1.5 609,952 + +0.4 609,952 + -0.2 609,954 + -1.2 609,955 - -1.4 609,955 - -0.1 609,956 + +0.6 609,956 + -0.3
Or see this region's nucleotide sequence