Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02340 and MMP_RS02345 are separated by 31 nucleotides MMP_RS02345 and MMP_RS02350 are separated by 6 nucleotides MMP_RS02350 and MMP_RS02355 are separated by 1 nucleotides MMP_RS02355 and MMP_RS02360 are separated by 97 nucleotides MMP_RS02360 and MMP_RS02365 are separated by 206 nucleotides
MMP_RS02340: MMP_RS02340 - DNA-directed RNA polymerase subunit E'', at 440,245 to 440,421
_RS02340
MMP_RS02345: MMP_RS02345 - GTP-dependent dephospho-CoA kinase family protein, at 440,453 to 440,929
_RS02345
MMP_RS02350: MMP_RS02350 - 30S ribosomal protein S24e, at 440,936 to 441,244
_RS02350
MMP_RS02355: MMP_RS02355 - 30S ribosomal protein S27ae, at 441,246 to 441,449
_RS02355
MMP_RS02360: MMP_RS02360 - hypothetical protein, at 441,547 to 441,783
_RS02360
MMP_RS02365: MMP_RS02365 - nitrogen fixation, at 441,990 to 442,316
_RS02365
Position (kb) 441
442 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 440.337 kb on + strand, within MMP_RS02340 at 440.390 kb on - strand, within MMP_RS02340 at 440.391 kb on + strand, within MMP_RS02340 at 440.401 kb on - strand, within MMP_RS02340 at 440.415 kb on - strand at 440.420 kb on + strand at 440.439 kb on - strand at 440.441 kb on - strand at 440.441 kb on - strand at 440.457 kb on + strand at 440.460 kb on - strand at 440.475 kb on - strand at 440.530 kb on + strand, within MMP_RS02345 at 440.587 kb on - strand, within MMP_RS02345 at 440.587 kb on - strand, within MMP_RS02345 at 440.587 kb on - strand, within MMP_RS02345 at 440.601 kb on - strand, within MMP_RS02345 at 440.740 kb on + strand, within MMP_RS02345 at 440.808 kb on + strand, within MMP_RS02345 at 440.951 kb on + strand at 440.966 kb on + strand at 441.254 kb on - strand at 441.292 kb on + strand, within MMP_RS02355 at 441.316 kb on - strand, within MMP_RS02355 at 441.359 kb on + strand, within MMP_RS02355 at 441.465 kb on + strand at 441.507 kb on + strand at 441.527 kb on - strand at 441.538 kb on - strand at 441.538 kb on - strand at 441.544 kb on - strand at 441.545 kb on + strand at 441.548 kb on - strand at 441.602 kb on - strand, within MMP_RS02360 at 441.602 kb on - strand, within MMP_RS02360 at 441.602 kb on - strand, within MMP_RS02360 at 441.603 kb on + strand, within MMP_RS02360 at 441.604 kb on - strand, within MMP_RS02360 at 441.604 kb on - strand, within MMP_RS02360 at 441.605 kb on + strand, within MMP_RS02360 at 441.799 kb on + strand at 441.801 kb on + strand at 441.812 kb on - strand at 441.827 kb on + strand at 441.840 kb on - strand at 441.841 kb on + strand at 441.847 kb on - strand at 441.859 kb on - strand at 441.859 kb on - strand at 441.878 kb on - strand at 441.879 kb on + strand at 441.996 kb on + strand at 442.017 kb on + strand at 442.020 kb on - strand at 442.046 kb on - strand, within MMP_RS02365 at 442.068 kb on - strand, within MMP_RS02365 at 442.068 kb on - strand, within MMP_RS02365 at 442.069 kb on + strand, within MMP_RS02365 at 442.120 kb on - strand, within MMP_RS02365 at 442.121 kb on + strand, within MMP_RS02365 at 442.121 kb on + strand, within MMP_RS02365 at 442.121 kb on + strand, within MMP_RS02365 at 442.122 kb on - strand, within MMP_RS02365 at 442.123 kb on + strand, within MMP_RS02365 at 442.123 kb on + strand, within MMP_RS02365 at 442.128 kb on - strand, within MMP_RS02365 at 442.129 kb on + strand, within MMP_RS02365 at 442.219 kb on + strand, within MMP_RS02365 at 442.219 kb on + strand, within MMP_RS02365 at 442.248 kb on - strand, within MMP_RS02365 at 442.297 kb on + strand at 442.361 kb on - strand at 442.361 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.422 kb on - strand at 442.423 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 440,337 + MMP_RS02340 0.52 -2.0 440,390 - MMP_RS02340 0.82 -1.7 440,391 + MMP_RS02340 0.82 -3.2 440,401 - MMP_RS02340 0.88 -3.6 440,415 - -4.7 440,420 + -2.4 440,439 - -3.6 440,441 - -5.7 440,441 - -3.7 440,457 + -4.1 440,460 - -2.9 440,475 - -3.5 440,530 + MMP_RS02345 0.16 -1.9 440,587 - MMP_RS02345 0.28 -1.6 440,587 - MMP_RS02345 0.28 -3.7 440,587 - MMP_RS02345 0.28 -4.8 440,601 - MMP_RS02345 0.31 -2.0 440,740 + MMP_RS02345 0.60 -1.8 440,808 + MMP_RS02345 0.74 -2.5 440,951 + -1.7 440,966 + +0.3 441,254 - -2.9 441,292 + MMP_RS02355 0.23 -1.8 441,316 - MMP_RS02355 0.34 -3.9 441,359 + MMP_RS02355 0.55 -2.8 441,465 + -1.1 441,507 + +3.1 441,527 - -0.6 441,538 - +0.6 441,538 - +0.3 441,544 - -0.2 441,545 + +0.5 441,548 - -0.9 441,602 - MMP_RS02360 0.23 +0.3 441,602 - MMP_RS02360 0.23 -0.7 441,602 - MMP_RS02360 0.23 +0.4 441,603 + MMP_RS02360 0.24 +0.2 441,604 - MMP_RS02360 0.24 +0.6 441,604 - MMP_RS02360 0.24 -0.5 441,605 + MMP_RS02360 0.24 +0.1 441,799 + -0.2 441,801 + -0.3 441,812 - +0.6 441,827 + +3.4 441,840 - -0.7 441,841 + +0.1 441,847 - +0.5 441,859 - +0.7 441,859 - +0.4 441,878 - +3.2 441,879 + -0.1 441,996 + +0.4 442,017 + +0.3 442,020 - +0.4 442,046 - MMP_RS02365 0.17 +0.3 442,068 - MMP_RS02365 0.24 +0.7 442,068 - MMP_RS02365 0.24 -0.3 442,069 + MMP_RS02365 0.24 -0.0 442,120 - MMP_RS02365 0.40 -0.3 442,121 + MMP_RS02365 0.40 +0.3 442,121 + MMP_RS02365 0.40 +0.0 442,121 + MMP_RS02365 0.40 +0.5 442,122 - MMP_RS02365 0.40 +2.1 442,123 + MMP_RS02365 0.41 -0.3 442,123 + MMP_RS02365 0.41 +0.1 442,128 - MMP_RS02365 0.42 +0.1 442,129 + MMP_RS02365 0.43 +3.5 442,219 + MMP_RS02365 0.70 +0.8 442,219 + MMP_RS02365 0.70 +0.6 442,248 - MMP_RS02365 0.79 +0.3 442,297 + -0.4 442,361 - -1.3 442,361 - +0.2 442,418 - +3.6 442,418 - -0.8 442,418 - -1.0 442,418 - +3.8 442,418 - +0.7 442,418 - +0.2 442,418 - +0.1 442,418 - +0.1 442,418 - +0.7 442,418 - -0.3 442,418 - +0.1 442,418 - -0.1 442,418 - +0.3 442,418 - -0.5 442,419 + +0.2 442,419 + -0.7 442,419 + +0.2 442,419 + +0.3 442,419 + +0.1 442,419 + -1.1 442,422 - -0.3 442,423 + -0.0
Or see this region's nucleotide sequence