Experiment: Recovery broth with Vancomycin from Streptomyces orientalis 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16605 and BBR_RS16610 are separated by 64 nucleotides BBR_RS16610 and BBR_RS16615 are separated by 11 nucleotides BBR_RS16615 and BBR_RS16620 overlap by 11 nucleotides BBR_RS16620 and BBR_RS16625 are separated by 15 nucleotides
BBR_RS16605: BBR_RS16605 - hypothetical protein, at 1,493,206 to 1,495,818
_RS16605
BBR_RS16610: BBR_RS16610 - hypothetical protein, at 1,495,883 to 1,496,467
_RS16610
BBR_RS16615: BBR_RS16615 - class I SAM-dependent RNA methyltransferase, at 1,496,479 to 1,497,738
_RS16615
BBR_RS16620: BBR_RS16620 - DUF3159 domain-containing protein, at 1,497,728 to 1,498,504
_RS16620
BBR_RS16625: BBR_RS16625 - DUF3710 domain-containing protein, at 1,498,520 to 1,499,365
_RS16625
Position (kb)
1496
1497
1498 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1495.517 kb on + strand, within BBR_RS16605 at 1495.538 kb on + strand, within BBR_RS16605 at 1495.538 kb on + strand, within BBR_RS16605 at 1495.538 kb on + strand, within BBR_RS16605 at 1495.546 kb on - strand, within BBR_RS16605 at 1495.660 kb on - strand at 1495.687 kb on + strand at 1495.704 kb on + strand at 1495.772 kb on - strand at 1495.816 kb on + strand at 1495.819 kb on - strand at 1495.910 kb on + strand at 1495.910 kb on + strand at 1495.962 kb on + strand, within BBR_RS16610 at 1496.003 kb on + strand, within BBR_RS16610 at 1496.009 kb on - strand, within BBR_RS16610 at 1496.023 kb on - strand, within BBR_RS16610 at 1496.053 kb on - strand, within BBR_RS16610 at 1496.075 kb on + strand, within BBR_RS16610 at 1496.086 kb on + strand, within BBR_RS16610 at 1496.104 kb on - strand, within BBR_RS16610 at 1496.235 kb on + strand, within BBR_RS16610 at 1496.253 kb on - strand, within BBR_RS16610 at 1496.377 kb on - strand, within BBR_RS16610 at 1496.412 kb on + strand at 1496.472 kb on - strand at 1496.475 kb on + strand at 1496.561 kb on - strand at 1496.565 kb on + strand at 1496.569 kb on + strand at 1496.623 kb on - strand, within BBR_RS16615 at 1496.689 kb on + strand, within BBR_RS16615 at 1496.719 kb on + strand, within BBR_RS16615 at 1496.719 kb on + strand, within BBR_RS16615 at 1496.741 kb on + strand, within BBR_RS16615 at 1496.741 kb on + strand, within BBR_RS16615 at 1496.741 kb on + strand, within BBR_RS16615 at 1496.749 kb on - strand, within BBR_RS16615 at 1496.749 kb on - strand, within BBR_RS16615 at 1496.759 kb on - strand, within BBR_RS16615 at 1496.823 kb on + strand, within BBR_RS16615 at 1496.823 kb on + strand, within BBR_RS16615 at 1496.850 kb on - strand, within BBR_RS16615 at 1496.856 kb on + strand, within BBR_RS16615 at 1496.875 kb on - strand, within BBR_RS16615 at 1496.917 kb on + strand, within BBR_RS16615 at 1496.917 kb on + strand, within BBR_RS16615 at 1496.917 kb on + strand, within BBR_RS16615 at 1496.936 kb on + strand, within BBR_RS16615 at 1496.944 kb on - strand, within BBR_RS16615 at 1496.944 kb on - strand, within BBR_RS16615 at 1496.969 kb on + strand, within BBR_RS16615 at 1497.014 kb on + strand, within BBR_RS16615 at 1497.060 kb on + strand, within BBR_RS16615 at 1497.075 kb on + strand, within BBR_RS16615 at 1497.091 kb on - strand, within BBR_RS16615 at 1497.145 kb on + strand, within BBR_RS16615 at 1497.155 kb on + strand, within BBR_RS16615 at 1497.191 kb on - strand, within BBR_RS16615 at 1497.214 kb on - strand, within BBR_RS16615 at 1497.264 kb on - strand, within BBR_RS16615 at 1497.312 kb on + strand, within BBR_RS16615 at 1497.348 kb on - strand, within BBR_RS16615 at 1497.412 kb on - strand, within BBR_RS16615 at 1497.497 kb on + strand, within BBR_RS16615 at 1497.527 kb on + strand, within BBR_RS16615 at 1497.535 kb on - strand, within BBR_RS16615 at 1497.555 kb on - strand, within BBR_RS16615 at 1497.584 kb on + strand, within BBR_RS16615 at 1497.584 kb on + strand, within BBR_RS16615 at 1497.621 kb on + strand at 1497.629 kb on - strand at 1497.681 kb on - strand at 1497.692 kb on - strand at 1497.697 kb on + strand at 1497.746 kb on - strand at 1497.903 kb on + strand, within BBR_RS16620 at 1497.911 kb on - strand, within BBR_RS16620 at 1497.925 kb on - strand, within BBR_RS16620 at 1497.959 kb on + strand, within BBR_RS16620 at 1498.012 kb on - strand, within BBR_RS16620 at 1498.012 kb on - strand, within BBR_RS16620 at 1498.041 kb on - strand, within BBR_RS16620 at 1498.116 kb on - strand, within BBR_RS16620 at 1498.193 kb on - strand, within BBR_RS16620 at 1498.221 kb on - strand, within BBR_RS16620 at 1498.240 kb on - strand, within BBR_RS16620 at 1498.267 kb on + strand, within BBR_RS16620 at 1498.388 kb on - strand, within BBR_RS16620 at 1498.483 kb on - strand at 1498.513 kb on + strand at 1498.596 kb on + strand at 1498.646 kb on - strand, within BBR_RS16625 at 1498.727 kb on - strand, within BBR_RS16625
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Vancomycin from Streptomyces orientalis 2e-04 mM remove 1,495,517 + BBR_RS16605 0.88 +0.5 1,495,538 + BBR_RS16605 0.89 +0.6 1,495,538 + BBR_RS16605 0.89 -0.3 1,495,538 + BBR_RS16605 0.89 -1.4 1,495,546 - BBR_RS16605 0.90 -0.9 1,495,660 - -0.2 1,495,687 + +0.9 1,495,704 + +0.8 1,495,772 - +0.3 1,495,816 + -0.9 1,495,819 - -2.1 1,495,910 + +0.6 1,495,910 + -0.4 1,495,962 + BBR_RS16610 0.14 +0.1 1,496,003 + BBR_RS16610 0.21 +0.0 1,496,009 - BBR_RS16610 0.22 -0.0 1,496,023 - BBR_RS16610 0.24 -0.6 1,496,053 - BBR_RS16610 0.29 -0.7 1,496,075 + BBR_RS16610 0.33 -0.2 1,496,086 + BBR_RS16610 0.35 -0.5 1,496,104 - BBR_RS16610 0.38 -0.0 1,496,235 + BBR_RS16610 0.60 +1.2 1,496,253 - BBR_RS16610 0.63 -0.9 1,496,377 - BBR_RS16610 0.84 +0.7 1,496,412 + -0.5 1,496,472 - -0.3 1,496,475 + -0.1 1,496,561 - +1.8 1,496,565 + +0.5 1,496,569 + +0.7 1,496,623 - BBR_RS16615 0.11 -0.2 1,496,689 + BBR_RS16615 0.17 -0.5 1,496,719 + BBR_RS16615 0.19 +1.3 1,496,719 + BBR_RS16615 0.19 -1.6 1,496,741 + BBR_RS16615 0.21 +0.6 1,496,741 + BBR_RS16615 0.21 -0.2 1,496,741 + BBR_RS16615 0.21 +1.2 1,496,749 - BBR_RS16615 0.21 -0.5 1,496,749 - BBR_RS16615 0.21 -1.3 1,496,759 - BBR_RS16615 0.22 -0.7 1,496,823 + BBR_RS16615 0.27 -0.2 1,496,823 + BBR_RS16615 0.27 +0.9 1,496,850 - BBR_RS16615 0.29 +0.4 1,496,856 + BBR_RS16615 0.30 -0.9 1,496,875 - BBR_RS16615 0.31 -1.2 1,496,917 + BBR_RS16615 0.35 -0.8 1,496,917 + BBR_RS16615 0.35 +0.5 1,496,917 + BBR_RS16615 0.35 -1.2 1,496,936 + BBR_RS16615 0.36 +2.3 1,496,944 - BBR_RS16615 0.37 -0.4 1,496,944 - BBR_RS16615 0.37 -0.3 1,496,969 + BBR_RS16615 0.39 +0.7 1,497,014 + BBR_RS16615 0.42 +0.5 1,497,060 + BBR_RS16615 0.46 -1.6 1,497,075 + BBR_RS16615 0.47 -0.2 1,497,091 - BBR_RS16615 0.49 -0.0 1,497,145 + BBR_RS16615 0.53 -0.0 1,497,155 + BBR_RS16615 0.54 +0.3 1,497,191 - BBR_RS16615 0.57 +0.1 1,497,214 - BBR_RS16615 0.58 -1.4 1,497,264 - BBR_RS16615 0.62 -1.8 1,497,312 + BBR_RS16615 0.66 -0.8 1,497,348 - BBR_RS16615 0.69 +1.0 1,497,412 - BBR_RS16615 0.74 +0.1 1,497,497 + BBR_RS16615 0.81 -0.4 1,497,527 + BBR_RS16615 0.83 +1.2 1,497,535 - BBR_RS16615 0.84 -0.2 1,497,555 - BBR_RS16615 0.85 +0.3 1,497,584 + BBR_RS16615 0.88 -0.3 1,497,584 + BBR_RS16615 0.88 -0.0 1,497,621 + +2.9 1,497,629 - +0.3 1,497,681 - -0.7 1,497,692 - -1.0 1,497,697 + -0.2 1,497,746 - +2.0 1,497,903 + BBR_RS16620 0.23 +0.1 1,497,911 - BBR_RS16620 0.24 +0.0 1,497,925 - BBR_RS16620 0.25 +1.7 1,497,959 + BBR_RS16620 0.30 +1.1 1,498,012 - BBR_RS16620 0.37 -0.4 1,498,012 - BBR_RS16620 0.37 +0.0 1,498,041 - BBR_RS16620 0.40 +0.8 1,498,116 - BBR_RS16620 0.50 -0.4 1,498,193 - BBR_RS16620 0.60 +0.5 1,498,221 - BBR_RS16620 0.63 -0.7 1,498,240 - BBR_RS16620 0.66 -0.4 1,498,267 + BBR_RS16620 0.69 +0.4 1,498,388 - BBR_RS16620 0.85 -0.0 1,498,483 - +1.4 1,498,513 + -0.5 1,498,596 + -0.1 1,498,646 - BBR_RS16625 0.15 -1.3 1,498,727 - BBR_RS16625 0.24 +1.0
Or see this region's nucleotide sequence