Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_563

Experiment: Chloride 300 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_562 and Pf1N1B4_563 overlap by 4 nucleotidesPf1N1B4_563 and Pf1N1B4_564 are separated by 233 nucleotides Pf1N1B4_562 - Histidine transporter, periplasmic histidine-binding protein, at 658,797 to 659,810 _562 Pf1N1B4_563 - RNA polymerase sigma-70 factor, at 659,807 to 660,355 _563 Pf1N1B4_564 - ABC-type phosphate/phosphonate transport system, periplasmic component, at 660,589 to 661,353 _564 Position (kb) 659 660 661Strain fitness (log2 ratio) -2 -1 0 1 2at 659.165 kb on - strand, within Pf1N1B4_562at 659.165 kb on - strand, within Pf1N1B4_562at 659.248 kb on + strand, within Pf1N1B4_562at 659.248 kb on + strand, within Pf1N1B4_562at 659.248 kb on + strand, within Pf1N1B4_562at 659.249 kb on - strand, within Pf1N1B4_562at 659.249 kb on - strand, within Pf1N1B4_562at 659.266 kb on + strandat 659.267 kb on - strand, within Pf1N1B4_562at 659.267 kb on - strand, within Pf1N1B4_562at 659.418 kb on - strand, within Pf1N1B4_562at 659.449 kb on + strand, within Pf1N1B4_562at 659.449 kb on + strand, within Pf1N1B4_562at 659.449 kb on + strand, within Pf1N1B4_562at 659.450 kb on - strand, within Pf1N1B4_562at 659.450 kb on - strand, within Pf1N1B4_562at 659.450 kb on - strand, within Pf1N1B4_562at 659.450 kb on - strand, within Pf1N1B4_562at 659.892 kb on + strand, within Pf1N1B4_563at 659.892 kb on + strand, within Pf1N1B4_563at 659.892 kb on + strand, within Pf1N1B4_563at 659.892 kb on + strand, within Pf1N1B4_563at 659.893 kb on - strand, within Pf1N1B4_563at 659.893 kb on - strand, within Pf1N1B4_563at 659.943 kb on + strand, within Pf1N1B4_563at 659.943 kb on + strand, within Pf1N1B4_563at 660.138 kb on + strand, within Pf1N1B4_563at 660.355 kb on + strandat 660.355 kb on + strandat 660.355 kb on + strandat 660.355 kb on + strandat 660.355 kb on + strandat 660.356 kb on - strandat 660.356 kb on - strandat 660.412 kb on - strandat 660.412 kb on - strandat 660.412 kb on - strandat 660.412 kb on - strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.485 kb on + strandat 660.486 kb on - strandat 660.486 kb on - strandat 660.651 kb on - strandat 660.785 kb on + strand, within Pf1N1B4_564at 660.785 kb on + strand, within Pf1N1B4_564at 660.786 kb on - strand, within Pf1N1B4_564at 660.863 kb on + strand, within Pf1N1B4_564at 661.102 kb on + strand, within Pf1N1B4_564at 661.102 kb on + strand, within Pf1N1B4_564at 661.139 kb on - strand, within Pf1N1B4_564at 661.349 kb on + strandat 661.349 kb on + strandat 661.349 kb on + strandat 661.350 kb on - strandat 661.350 kb on - strandat 661.350 kb on - strandat 661.350 kb on - strandat 661.350 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloride 300 mM
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659,165 - Pf1N1B4_562 0.36 +0.2
659,165 - Pf1N1B4_562 0.36 -0.1
659,248 + Pf1N1B4_562 0.44 +0.6
659,248 + Pf1N1B4_562 0.44 -0.1
659,248 + Pf1N1B4_562 0.44 +0.9
659,249 - Pf1N1B4_562 0.45 -0.1
659,249 - Pf1N1B4_562 0.45 +0.3
659,266 + -1.1
659,267 - Pf1N1B4_562 0.46 -0.1
659,267 - Pf1N1B4_562 0.46 -0.7
659,418 - Pf1N1B4_562 0.61 -0.3
659,449 + Pf1N1B4_562 0.64 +1.5
659,449 + Pf1N1B4_562 0.64 -0.4
659,449 + Pf1N1B4_562 0.64 +0.8
659,450 - Pf1N1B4_562 0.64 +0.6
659,450 - Pf1N1B4_562 0.64 +0.2
659,450 - Pf1N1B4_562 0.64 -1.7
659,450 - Pf1N1B4_562 0.64 +1.9
659,892 + Pf1N1B4_563 0.15 +0.3
659,892 + Pf1N1B4_563 0.15 -1.4
659,892 + Pf1N1B4_563 0.15 -2.2
659,892 + Pf1N1B4_563 0.15 -0.4
659,893 - Pf1N1B4_563 0.16 -1.7
659,893 - Pf1N1B4_563 0.16 -1.7
659,943 + Pf1N1B4_563 0.25 -0.1
659,943 + Pf1N1B4_563 0.25 -0.8
660,138 + Pf1N1B4_563 0.60 +0.3
660,355 + -2.2
660,355 + -0.1
660,355 + +1.6
660,355 + -0.2
660,355 + +0.2
660,356 - +1.9
660,356 - +0.2
660,412 - +1.9
660,412 - -0.1
660,412 - -1.2
660,412 - +0.2
660,485 + +1.9
660,485 + +0.6
660,485 + -2.1
660,485 + -1.1
660,485 + -0.1
660,485 + -1.1
660,485 + -0.1
660,485 + +0.9
660,485 + +1.7
660,486 - +0.3
660,486 - -1.1
660,651 - +0.9
660,785 + Pf1N1B4_564 0.26 -0.1
660,785 + Pf1N1B4_564 0.26 +0.4
660,786 - Pf1N1B4_564 0.26 -1.1
660,863 + Pf1N1B4_564 0.36 +0.5
661,102 + Pf1N1B4_564 0.67 +0.6
661,102 + Pf1N1B4_564 0.67 -0.2
661,139 - Pf1N1B4_564 0.72 -1.4
661,349 + -1.4
661,349 + -0.7
661,349 + -0.4
661,350 - +0.6
661,350 - -1.3
661,350 - -1.1
661,350 - -0.5
661,350 - +0.6

Or see this region's nucleotide sequence