Experiment: Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4952 and Pf1N1B4_4953 overlap by 4 nucleotides Pf1N1B4_4953 and Pf1N1B4_4954 are separated by 891 nucleotides
Pf1N1B4_4952 - L-seryl-tRNA(Sec) selenium transferase (EC 2.9.1.1), at 5,488,640 to 5,490,070
_4952
Pf1N1B4_4953 - Selenocysteine-specific translation elongation factor, at 5,490,067 to 5,491,986
_4953
Pf1N1B4_4954 - Enoyl-[acyl-carrier-protein] reductase [NADPH] (EC 1.3.1.10), at 5,492,878 to 5,493,624
_4954
Position (kb)
5490
5491
5492 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5489.560 kb on - strand, within Pf1N1B4_4952 at 5489.779 kb on - strand, within Pf1N1B4_4952 at 5489.779 kb on - strand, within Pf1N1B4_4952 at 5490.191 kb on - strand at 5490.192 kb on + strand at 5490.192 kb on + strand at 5490.193 kb on - strand at 5490.196 kb on + strand at 5490.393 kb on + strand, within Pf1N1B4_4953 at 5491.182 kb on - strand, within Pf1N1B4_4953 at 5491.517 kb on + strand, within Pf1N1B4_4953 at 5491.518 kb on - strand, within Pf1N1B4_4953 at 5491.617 kb on - strand, within Pf1N1B4_4953 at 5491.898 kb on - strand at 5492.113 kb on - strand at 5492.116 kb on + strand at 5492.131 kb on + strand at 5492.354 kb on + strand at 5492.355 kb on - strand at 5492.416 kb on - strand at 5492.417 kb on - strand at 5492.451 kb on - strand at 5492.472 kb on + strand at 5492.473 kb on - strand at 5492.565 kb on - strand at 5492.593 kb on + strand at 5492.594 kb on - strand at 5492.627 kb on - strand at 5492.729 kb on - strand at 5492.729 kb on - strand at 5492.813 kb on - strand at 5492.853 kb on + strand at 5492.854 kb on - strand at 5492.854 kb on - strand at 5492.854 kb on - strand at 5492.854 kb on - strand at 5492.854 kb on - strand at 5492.880 kb on - strand at 5492.904 kb on + strand at 5492.905 kb on - strand at 5492.905 kb on - strand at 5492.961 kb on + strand, within Pf1N1B4_4954 at 5492.965 kb on + strand, within Pf1N1B4_4954
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 300 mM remove 5,489,560 - Pf1N1B4_4952 0.64 +0.5 5,489,779 - Pf1N1B4_4952 0.80 +0.2 5,489,779 - Pf1N1B4_4952 0.80 -2.0 5,490,191 - +0.5 5,490,192 + -0.5 5,490,192 + +0.5 5,490,193 - +1.1 5,490,196 + +0.5 5,490,393 + Pf1N1B4_4953 0.17 +0.3 5,491,182 - Pf1N1B4_4953 0.58 -1.8 5,491,517 + Pf1N1B4_4953 0.76 -1.5 5,491,518 - Pf1N1B4_4953 0.76 -0.6 5,491,617 - Pf1N1B4_4953 0.81 -1.3 5,491,898 - +1.1 5,492,113 - -0.5 5,492,116 + -0.5 5,492,131 + +1.1 5,492,354 + -0.1 5,492,355 - -0.8 5,492,416 - +1.0 5,492,417 - -1.6 5,492,451 - +0.3 5,492,472 + -0.2 5,492,473 - -0.4 5,492,565 - -0.1 5,492,593 + +1.0 5,492,594 - -0.0 5,492,627 - +1.8 5,492,729 - +0.4 5,492,729 - +1.1 5,492,813 - -0.0 5,492,853 + +1.4 5,492,854 - +0.3 5,492,854 - -0.1 5,492,854 - +0.6 5,492,854 - +0.3 5,492,854 - -0.4 5,492,880 - -1.4 5,492,904 + -0.2 5,492,905 - -1.9 5,492,905 - -0.7 5,492,961 + Pf1N1B4_4954 0.11 +0.6 5,492,965 + Pf1N1B4_4954 0.12 -1.8
Or see this region's nucleotide sequence