Experiment: Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4704 and Pf1N1B4_4705 are separated by 267 nucleotides Pf1N1B4_4705 and Pf1N1B4_4706 are separated by 2 nucleotides Pf1N1B4_4706 and Pf1N1B4_4707 are separated by 122 nucleotides Pf1N1B4_4707 and Pf1N1B4_4708 are separated by 165 nucleotides
Pf1N1B4_4704 - hypothetical protein, at 5,198,896 to 5,203,722
_4704
Pf1N1B4_4705 - Delta 1-piperideine-2-carboxylate reductase (EC 1.5.1.21) / Delta 1-pyrroline-2-carboxylate reductase (EC 1.5.1.1), at 5,203,990 to 5,204,832
_4705
Pf1N1B4_4706 - Delta 1-piperideine-2-carboxylate reductase (EC 1.5.1.21) / Delta 1-pyrroline-2-carboxylate reductase (EC 1.5.1.1), at 5,204,835 to 5,205,029
_4706
Pf1N1B4_4707 - Probable transmembrane protein, at 5,205,152 to 5,205,469
_4707
Pf1N1B4_4708 - FIG00958097: hypothetical protein, at 5,205,635 to 5,205,886
_4708
Position (kb)
5203
5204
5205 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5203.454 kb on + strand at 5203.455 kb on - strand at 5203.779 kb on - strand at 5203.806 kb on - strand at 5203.809 kb on + strand at 5203.813 kb on + strand at 5203.971 kb on - strand at 5204.012 kb on + strand at 5204.013 kb on - strand at 5204.016 kb on + strand at 5204.016 kb on + strand at 5204.364 kb on + strand, within Pf1N1B4_4705 at 5204.364 kb on + strand, within Pf1N1B4_4705 at 5204.583 kb on - strand, within Pf1N1B4_4705 at 5204.583 kb on - strand, within Pf1N1B4_4705 at 5204.583 kb on - strand, within Pf1N1B4_4705 at 5204.707 kb on - strand, within Pf1N1B4_4705 at 5204.738 kb on + strand, within Pf1N1B4_4705 at 5205.137 kb on + strand at 5205.137 kb on + strand at 5205.234 kb on + strand at 5205.475 kb on + strand at 5205.475 kb on + strand at 5205.476 kb on - strand at 5205.592 kb on + strand at 5205.592 kb on + strand at 5205.592 kb on + strand at 5205.592 kb on + strand at 5205.592 kb on + strand at 5205.593 kb on - strand at 5205.593 kb on - strand at 5205.593 kb on - strand at 5205.599 kb on + strand at 5205.600 kb on - strand at 5205.600 kb on - strand at 5205.611 kb on + strand at 5205.617 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 300 mM remove 5,203,454 + +0.3 5,203,455 - -0.3 5,203,779 - -1.0 5,203,806 - +3.1 5,203,809 + +0.5 5,203,813 + +0.2 5,203,971 - -0.0 5,204,012 + +1.5 5,204,013 - +0.2 5,204,016 + -0.3 5,204,016 + -0.0 5,204,364 + Pf1N1B4_4705 0.44 -1.5 5,204,364 + Pf1N1B4_4705 0.44 -0.7 5,204,583 - Pf1N1B4_4705 0.70 -0.3 5,204,583 - Pf1N1B4_4705 0.70 -1.5 5,204,583 - Pf1N1B4_4705 0.70 -2.5 5,204,707 - Pf1N1B4_4705 0.85 -2.8 5,204,738 + Pf1N1B4_4705 0.89 +1.0 5,205,137 + -1.5 5,205,137 + -0.0 5,205,234 + -1.8 5,205,475 + +0.7 5,205,475 + +0.5 5,205,476 - +0.2 5,205,592 + -1.0 5,205,592 + +2.1 5,205,592 + -1.8 5,205,592 + +1.0 5,205,592 + +0.2 5,205,593 - -0.8 5,205,593 - -0.9 5,205,593 - -2.8 5,205,599 + +1.1 5,205,600 - -0.0 5,205,600 - -0.6 5,205,611 + -1.0 5,205,617 - +0.2
Or see this region's nucleotide sequence