Experiment: Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4585 and Pf1N1B4_4586 are separated by 73 nucleotides Pf1N1B4_4586 and Pf1N1B4_4587 are separated by 35 nucleotides Pf1N1B4_4587 and Pf1N1B4_4588 are separated by 112 nucleotides
Pf1N1B4_4585 - Putative Isoquinoline 1-oxidoreductase subunit, Mll3835 protein, at 5,056,612 to 5,057,190
_4585
Pf1N1B4_4586 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 5,057,264 to 5,057,872
_4586
Pf1N1B4_4587 - probable dioxygenase, at 5,057,908 to 5,058,333
_4587
Pf1N1B4_4588 - Transcriptional regulator, at 5,058,446 to 5,059,312
_4588
Position (kb)
5057
5058
5059 Strain fitness (log2 ratio)
-2
-1
0
1 at 5057.185 kb on - strand at 5057.236 kb on - strand at 5057.236 kb on - strand at 5057.759 kb on + strand, within Pf1N1B4_4586 at 5057.842 kb on + strand at 5058.126 kb on + strand, within Pf1N1B4_4587 at 5058.126 kb on + strand, within Pf1N1B4_4587 at 5058.126 kb on + strand, within Pf1N1B4_4587 at 5058.127 kb on - strand, within Pf1N1B4_4587 at 5058.270 kb on - strand, within Pf1N1B4_4587 at 5058.270 kb on - strand at 5058.371 kb on + strand at 5058.445 kb on + strand at 5058.445 kb on + strand at 5058.446 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 300 mM remove 5,057,185 - -0.2 5,057,236 - +1.6 5,057,236 - +1.0 5,057,759 + Pf1N1B4_4586 0.81 +0.6 5,057,842 + -0.2 5,058,126 + Pf1N1B4_4587 0.51 -2.2 5,058,126 + Pf1N1B4_4587 0.51 -0.1 5,058,126 + Pf1N1B4_4587 0.51 -1.7 5,058,127 - Pf1N1B4_4587 0.51 -1.0 5,058,270 - Pf1N1B4_4587 0.85 -0.8 5,058,270 - +0.0 5,058,371 + -1.4 5,058,445 + -0.4 5,058,445 + -1.0 5,058,446 - -1.0
Or see this region's nucleotide sequence