Experiment: Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3773 and Pf1N1B4_3774 are separated by 77 nucleotides Pf1N1B4_3774 and Pf1N1B4_3775 are separated by 75 nucleotides Pf1N1B4_3775 and Pf1N1B4_3776 are separated by 131 nucleotides Pf1N1B4_3776 and Pf1N1B4_3777 are separated by 238 nucleotides
Pf1N1B4_3773 - GTP cyclohydrolase I (EC 3.5.4.16) type 1, at 4,183,521 to 4,184,066
_3773
Pf1N1B4_3774 - Smr domain protein, at 4,184,144 to 4,184,701
_3774
Pf1N1B4_3775 - FIG00956876: hypothetical protein, at 4,184,777 to 4,185,097
_3775
Pf1N1B4_3776 - Isochorismatase (EC 3.3.2.1), at 4,185,229 to 4,185,819
_3776
Pf1N1B4_3777 - Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p, at 4,186,058 to 4,186,966
_3777
Position (kb)
4184
4185
4186 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4184.187 kb on + strand at 4184.331 kb on - strand, within Pf1N1B4_3774 at 4184.331 kb on - strand, within Pf1N1B4_3774 at 4184.872 kb on - strand, within Pf1N1B4_3775 at 4184.872 kb on - strand, within Pf1N1B4_3775 at 4184.872 kb on - strand, within Pf1N1B4_3775 at 4185.893 kb on + strand at 4185.893 kb on + strand at 4185.894 kb on - strand at 4185.947 kb on - strand at 4185.947 kb on - strand at 4185.963 kb on - strand at 4185.973 kb on + strand at 4185.973 kb on + strand at 4185.974 kb on - strand at 4185.974 kb on - strand at 4185.974 kb on - strand at 4186.023 kb on + strand at 4186.094 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 300 mM remove 4,184,187 + -1.2 4,184,331 - Pf1N1B4_3774 0.34 +0.0 4,184,331 - Pf1N1B4_3774 0.34 -1.2 4,184,872 - Pf1N1B4_3775 0.30 +1.6 4,184,872 - Pf1N1B4_3775 0.30 -0.1 4,184,872 - Pf1N1B4_3775 0.30 +0.0 4,185,893 + -1.0 4,185,893 + -1.2 4,185,894 - +1.7 4,185,947 - -0.3 4,185,947 - +1.2 4,185,963 - -1.1 4,185,973 + +0.8 4,185,973 + -1.5 4,185,974 - -2.6 4,185,974 - -1.5 4,185,974 - -1.8 4,186,023 + +1.8 4,186,094 - +0.2
Or see this region's nucleotide sequence