Experiment: Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3258 and Pf1N1B4_3259 are separated by 234 nucleotides Pf1N1B4_3259 and Pf1N1B4_3260 are separated by 1 nucleotides Pf1N1B4_3260 and Pf1N1B4_3261 are separated by 239 nucleotides
Pf1N1B4_3258 - FIG00953409: hypothetical protein, at 3,647,424 to 3,647,753
_3258
Pf1N1B4_3259 - FIG00956006: hypothetical protein, at 3,647,988 to 3,648,578
_3259
Pf1N1B4_3260 - Cardiolipin synthetase (EC 2.7.8.-), at 3,648,580 to 3,649,737
_3260
Pf1N1B4_3261 - Periplasmic beta-glucosidase (EC 3.2.1.21), at 3,649,977 to 3,652,268
_3261
Position (kb)
3648
3649
3650 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3647.583 kb on + strand, within Pf1N1B4_3258 at 3647.584 kb on - strand, within Pf1N1B4_3258 at 3647.619 kb on + strand, within Pf1N1B4_3258 at 3647.620 kb on - strand, within Pf1N1B4_3258 at 3647.631 kb on + strand, within Pf1N1B4_3258 at 3647.632 kb on - strand, within Pf1N1B4_3258 at 3647.632 kb on - strand, within Pf1N1B4_3258 at 3647.632 kb on - strand, within Pf1N1B4_3258 at 3647.632 kb on - strand, within Pf1N1B4_3258 at 3647.699 kb on + strand, within Pf1N1B4_3258 at 3647.700 kb on - strand, within Pf1N1B4_3258 at 3647.813 kb on + strand at 3647.835 kb on - strand at 3647.970 kb on - strand at 3647.970 kb on - strand at 3647.988 kb on - strand at 3647.988 kb on - strand at 3647.988 kb on - strand at 3647.988 kb on - strand at 3648.108 kb on - strand, within Pf1N1B4_3259 at 3648.119 kb on + strand, within Pf1N1B4_3259 at 3648.302 kb on - strand, within Pf1N1B4_3259 at 3648.302 kb on - strand at 3648.387 kb on + strand, within Pf1N1B4_3259 at 3648.387 kb on + strand, within Pf1N1B4_3259 at 3648.387 kb on + strand, within Pf1N1B4_3259 at 3648.387 kb on + strand, within Pf1N1B4_3259 at 3648.387 kb on + strand, within Pf1N1B4_3259 at 3648.388 kb on - strand, within Pf1N1B4_3259 at 3648.388 kb on - strand, within Pf1N1B4_3259 at 3648.388 kb on - strand, within Pf1N1B4_3259 at 3648.388 kb on - strand, within Pf1N1B4_3259 at 3648.388 kb on - strand at 3648.388 kb on - strand, within Pf1N1B4_3259 at 3648.706 kb on + strand, within Pf1N1B4_3260 at 3648.707 kb on - strand, within Pf1N1B4_3260 at 3648.707 kb on - strand, within Pf1N1B4_3260 at 3648.802 kb on + strand, within Pf1N1B4_3260 at 3648.871 kb on + strand, within Pf1N1B4_3260 at 3648.871 kb on + strand at 3648.872 kb on - strand, within Pf1N1B4_3260 at 3649.189 kb on + strand, within Pf1N1B4_3260 at 3649.279 kb on + strand, within Pf1N1B4_3260 at 3649.390 kb on + strand, within Pf1N1B4_3260 at 3649.405 kb on + strand, within Pf1N1B4_3260 at 3649.409 kb on - strand, within Pf1N1B4_3260 at 3649.448 kb on - strand, within Pf1N1B4_3260 at 3649.764 kb on + strand at 3649.764 kb on + strand at 3649.764 kb on + strand at 3649.764 kb on + strand at 3649.764 kb on + strand at 3649.764 kb on + strand at 3649.765 kb on - strand at 3649.765 kb on - strand at 3649.765 kb on - strand at 3649.799 kb on + strand at 3649.799 kb on + strand at 3649.799 kb on + strand at 3649.799 kb on + strand at 3649.927 kb on - strand at 3649.972 kb on - strand at 3649.972 kb on - strand at 3649.978 kb on + strand at 3649.978 kb on + strand at 3649.978 kb on + strand at 3649.978 kb on + strand at 3649.978 kb on + strand at 3649.978 kb on + strand at 3649.979 kb on - strand at 3649.979 kb on - strand at 3649.979 kb on - strand at 3649.979 kb on - strand at 3650.077 kb on + strand at 3650.078 kb on - strand at 3650.143 kb on - strand at 3650.206 kb on + strand at 3650.206 kb on + strand at 3650.206 kb on + strand at 3650.206 kb on + strand at 3650.206 kb on + strand at 3650.206 kb on + strand at 3650.207 kb on - strand, within Pf1N1B4_3261 at 3650.207 kb on - strand, within Pf1N1B4_3261 at 3650.207 kb on - strand, within Pf1N1B4_3261 at 3650.207 kb on - strand, within Pf1N1B4_3261 at 3650.207 kb on - strand, within Pf1N1B4_3261 at 3650.207 kb on - strand, within Pf1N1B4_3261 at 3650.332 kb on + strand, within Pf1N1B4_3261 at 3650.333 kb on - strand, within Pf1N1B4_3261 at 3650.333 kb on - strand, within Pf1N1B4_3261 at 3650.333 kb on - strand, within Pf1N1B4_3261 at 3650.383 kb on + strand, within Pf1N1B4_3261 at 3650.395 kb on + strand, within Pf1N1B4_3261 at 3650.395 kb on + strand, within Pf1N1B4_3261 at 3650.395 kb on + strand, within Pf1N1B4_3261 at 3650.395 kb on + strand, within Pf1N1B4_3261 at 3650.395 kb on + strand, within Pf1N1B4_3261 at 3650.395 kb on + strand at 3650.396 kb on - strand, within Pf1N1B4_3261 at 3650.396 kb on - strand, within Pf1N1B4_3261 at 3650.396 kb on - strand, within Pf1N1B4_3261 at 3650.396 kb on - strand, within Pf1N1B4_3261 at 3650.396 kb on - strand, within Pf1N1B4_3261 at 3650.396 kb on - strand, within Pf1N1B4_3261 at 3650.396 kb on - strand, within Pf1N1B4_3261 at 3650.396 kb on - strand, within Pf1N1B4_3261 at 3650.396 kb on - strand, within Pf1N1B4_3261 at 3650.396 kb on - strand at 3650.396 kb on - strand, within Pf1N1B4_3261 at 3650.396 kb on - strand, within Pf1N1B4_3261 at 3650.446 kb on + strand, within Pf1N1B4_3261 at 3650.446 kb on + strand, within Pf1N1B4_3261 at 3650.447 kb on - strand, within Pf1N1B4_3261 at 3650.447 kb on - strand, within Pf1N1B4_3261 at 3650.450 kb on - strand at 3650.509 kb on + strand, within Pf1N1B4_3261 at 3650.510 kb on - strand, within Pf1N1B4_3261 at 3650.510 kb on - strand, within Pf1N1B4_3261 at 3650.510 kb on - strand, within Pf1N1B4_3261 at 3650.632 kb on - strand, within Pf1N1B4_3261
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 300 mM remove 3,647,583 + Pf1N1B4_3258 0.48 +1.0 3,647,584 - Pf1N1B4_3258 0.48 +1.5 3,647,619 + Pf1N1B4_3258 0.59 +1.0 3,647,620 - Pf1N1B4_3258 0.59 +0.5 3,647,631 + Pf1N1B4_3258 0.63 -0.1 3,647,632 - Pf1N1B4_3258 0.63 -1.4 3,647,632 - Pf1N1B4_3258 0.63 -1.6 3,647,632 - Pf1N1B4_3258 0.63 -1.9 3,647,632 - Pf1N1B4_3258 0.63 -0.5 3,647,699 + Pf1N1B4_3258 0.83 +1.0 3,647,700 - Pf1N1B4_3258 0.84 +0.7 3,647,813 + +1.3 3,647,835 - -1.3 3,647,970 - -0.0 3,647,970 - -0.3 3,647,988 - -0.4 3,647,988 - -0.4 3,647,988 - -0.4 3,647,988 - +0.7 3,648,108 - Pf1N1B4_3259 0.20 +1.0 3,648,119 + Pf1N1B4_3259 0.22 +1.0 3,648,302 - Pf1N1B4_3259 0.53 -0.3 3,648,302 - -0.4 3,648,387 + Pf1N1B4_3259 0.68 -0.3 3,648,387 + Pf1N1B4_3259 0.68 -0.6 3,648,387 + Pf1N1B4_3259 0.68 -0.6 3,648,387 + Pf1N1B4_3259 0.68 -0.3 3,648,387 + Pf1N1B4_3259 0.68 -2.2 3,648,388 - Pf1N1B4_3259 0.68 -0.8 3,648,388 - Pf1N1B4_3259 0.68 -1.2 3,648,388 - Pf1N1B4_3259 0.68 -1.5 3,648,388 - Pf1N1B4_3259 0.68 +1.0 3,648,388 - -0.6 3,648,388 - Pf1N1B4_3259 0.68 -0.6 3,648,706 + Pf1N1B4_3260 0.11 -0.6 3,648,707 - Pf1N1B4_3260 0.11 -0.9 3,648,707 - Pf1N1B4_3260 0.11 -1.4 3,648,802 + Pf1N1B4_3260 0.19 -1.4 3,648,871 + Pf1N1B4_3260 0.25 +1.5 3,648,871 + -0.6 3,648,872 - Pf1N1B4_3260 0.25 -0.0 3,649,189 + Pf1N1B4_3260 0.53 -1.9 3,649,279 + Pf1N1B4_3260 0.60 -1.0 3,649,390 + Pf1N1B4_3260 0.70 +1.0 3,649,405 + Pf1N1B4_3260 0.71 -1.2 3,649,409 - Pf1N1B4_3260 0.72 -1.9 3,649,448 - Pf1N1B4_3260 0.75 -0.6 3,649,764 + +1.4 3,649,764 + -1.0 3,649,764 + -0.0 3,649,764 + +1.0 3,649,764 + +0.3 3,649,764 + +0.2 3,649,765 - -0.4 3,649,765 - +0.5 3,649,765 - -0.3 3,649,799 + -0.6 3,649,799 + -2.0 3,649,799 + +1.0 3,649,799 + -0.0 3,649,927 - -0.4 3,649,972 - -1.9 3,649,972 - +2.0 3,649,978 + +1.8 3,649,978 + +0.2 3,649,978 + +1.0 3,649,978 + -0.2 3,649,978 + -0.0 3,649,978 + +0.1 3,649,979 - -1.0 3,649,979 - +0.2 3,649,979 - -1.1 3,649,979 - +0.4 3,650,077 + +0.1 3,650,078 - -1.7 3,650,143 - +1.8 3,650,206 + +0.5 3,650,206 + +1.0 3,650,206 + +0.2 3,650,206 + -1.0 3,650,206 + +0.3 3,650,206 + +1.7 3,650,207 - Pf1N1B4_3261 0.10 +0.1 3,650,207 - Pf1N1B4_3261 0.10 -2.0 3,650,207 - Pf1N1B4_3261 0.10 -0.2 3,650,207 - Pf1N1B4_3261 0.10 +0.8 3,650,207 - Pf1N1B4_3261 0.10 -0.6 3,650,207 - Pf1N1B4_3261 0.10 -1.6 3,650,332 + Pf1N1B4_3261 0.15 +0.4 3,650,333 - Pf1N1B4_3261 0.16 +0.2 3,650,333 - Pf1N1B4_3261 0.16 +0.7 3,650,333 - Pf1N1B4_3261 0.16 +1.3 3,650,383 + Pf1N1B4_3261 0.18 -0.0 3,650,395 + Pf1N1B4_3261 0.18 -0.6 3,650,395 + Pf1N1B4_3261 0.18 +0.5 3,650,395 + Pf1N1B4_3261 0.18 -0.0 3,650,395 + Pf1N1B4_3261 0.18 -0.4 3,650,395 + Pf1N1B4_3261 0.18 +0.8 3,650,395 + +1.0 3,650,396 - Pf1N1B4_3261 0.18 -1.0 3,650,396 - Pf1N1B4_3261 0.18 -0.0 3,650,396 - Pf1N1B4_3261 0.18 -1.6 3,650,396 - Pf1N1B4_3261 0.18 +1.6 3,650,396 - Pf1N1B4_3261 0.18 +1.8 3,650,396 - Pf1N1B4_3261 0.18 +0.7 3,650,396 - Pf1N1B4_3261 0.18 -0.6 3,650,396 - Pf1N1B4_3261 0.18 +1.0 3,650,396 - Pf1N1B4_3261 0.18 -0.2 3,650,396 - -0.6 3,650,396 - Pf1N1B4_3261 0.18 -1.4 3,650,396 - Pf1N1B4_3261 0.18 +0.2 3,650,446 + Pf1N1B4_3261 0.20 +0.0 3,650,446 + Pf1N1B4_3261 0.20 +1.0 3,650,447 - Pf1N1B4_3261 0.21 +2.4 3,650,447 - Pf1N1B4_3261 0.21 +1.3 3,650,450 - +0.4 3,650,509 + Pf1N1B4_3261 0.23 +1.0 3,650,510 - Pf1N1B4_3261 0.23 -0.3 3,650,510 - Pf1N1B4_3261 0.23 -0.0 3,650,510 - Pf1N1B4_3261 0.23 +1.5 3,650,632 - Pf1N1B4_3261 0.29 -0.9
Or see this region's nucleotide sequence