Experiment: Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2839 and Pf1N1B4_2840 are separated by 8 nucleotides Pf1N1B4_2840 and Pf1N1B4_2841 are separated by 1 nucleotides Pf1N1B4_2841 and Pf1N1B4_2842 are separated by 2 nucleotides
Pf1N1B4_2839 - Dephospho-CoA kinase (EC 2.7.1.24), at 3,183,777 to 3,184,388
_2839
Pf1N1B4_2840 - Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-), at 3,184,397 to 3,185,269
_2840
Pf1N1B4_2841 - Type IV fimbrial assembly protein PilC, at 3,185,271 to 3,186,488
_2841
Pf1N1B4_2842 - Type IV fimbrial assembly, ATPase PilB, at 3,186,491 to 3,188,191
_2842
Position (kb)
3185
3186
3187 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3184.395 kb on - strand at 3184.423 kb on - strand at 3184.423 kb on - strand at 3184.423 kb on - strand at 3184.423 kb on - strand at 3184.423 kb on - strand at 3184.434 kb on - strand at 3184.434 kb on - strand at 3184.480 kb on - strand at 3184.673 kb on + strand, within Pf1N1B4_2840 at 3185.001 kb on + strand, within Pf1N1B4_2840 at 3185.001 kb on + strand, within Pf1N1B4_2840 at 3185.002 kb on - strand at 3185.002 kb on - strand, within Pf1N1B4_2840 at 3185.002 kb on - strand, within Pf1N1B4_2840 at 3185.002 kb on - strand, within Pf1N1B4_2840 at 3185.002 kb on - strand, within Pf1N1B4_2840 at 3185.073 kb on - strand, within Pf1N1B4_2840 at 3185.082 kb on + strand, within Pf1N1B4_2840 at 3185.082 kb on + strand, within Pf1N1B4_2840 at 3185.082 kb on + strand, within Pf1N1B4_2840 at 3185.083 kb on - strand, within Pf1N1B4_2840 at 3185.083 kb on - strand, within Pf1N1B4_2840 at 3185.083 kb on - strand, within Pf1N1B4_2840 at 3185.083 kb on - strand, within Pf1N1B4_2840 at 3185.083 kb on - strand, within Pf1N1B4_2840 at 3185.269 kb on + strand at 3185.269 kb on + strand at 3185.270 kb on - strand at 3185.270 kb on - strand at 3185.270 kb on - strand at 3185.270 kb on - strand at 3185.270 kb on - strand at 3185.270 kb on - strand at 3185.305 kb on + strand at 3185.305 kb on + strand at 3185.306 kb on - strand at 3185.343 kb on + strand at 3185.344 kb on - strand at 3185.346 kb on + strand at 3185.346 kb on + strand at 3185.347 kb on - strand at 3185.347 kb on - strand at 3185.410 kb on + strand, within Pf1N1B4_2841 at 3185.410 kb on + strand, within Pf1N1B4_2841 at 3185.411 kb on - strand, within Pf1N1B4_2841 at 3185.411 kb on - strand, within Pf1N1B4_2841 at 3185.496 kb on + strand, within Pf1N1B4_2841 at 3185.496 kb on + strand, within Pf1N1B4_2841 at 3185.496 kb on + strand at 3185.496 kb on + strand, within Pf1N1B4_2841 at 3185.497 kb on - strand, within Pf1N1B4_2841 at 3185.497 kb on - strand, within Pf1N1B4_2841 at 3185.497 kb on - strand, within Pf1N1B4_2841 at 3185.497 kb on - strand, within Pf1N1B4_2841 at 3185.497 kb on - strand, within Pf1N1B4_2841 at 3185.497 kb on - strand, within Pf1N1B4_2841 at 3185.498 kb on + strand, within Pf1N1B4_2841 at 3185.498 kb on + strand, within Pf1N1B4_2841 at 3185.553 kb on + strand, within Pf1N1B4_2841 at 3185.553 kb on + strand, within Pf1N1B4_2841 at 3185.559 kb on + strand, within Pf1N1B4_2841 at 3185.559 kb on + strand, within Pf1N1B4_2841 at 3185.559 kb on + strand, within Pf1N1B4_2841 at 3185.559 kb on + strand at 3185.562 kb on + strand, within Pf1N1B4_2841 at 3185.562 kb on + strand, within Pf1N1B4_2841 at 3185.563 kb on - strand, within Pf1N1B4_2841 at 3185.686 kb on + strand, within Pf1N1B4_2841 at 3185.686 kb on + strand, within Pf1N1B4_2841 at 3185.687 kb on - strand, within Pf1N1B4_2841 at 3185.687 kb on - strand at 3185.687 kb on - strand, within Pf1N1B4_2841 at 3185.971 kb on + strand, within Pf1N1B4_2841 at 3185.971 kb on + strand, within Pf1N1B4_2841 at 3185.972 kb on - strand, within Pf1N1B4_2841 at 3186.022 kb on + strand, within Pf1N1B4_2841 at 3186.022 kb on + strand, within Pf1N1B4_2841 at 3186.022 kb on + strand, within Pf1N1B4_2841 at 3186.022 kb on + strand, within Pf1N1B4_2841 at 3186.023 kb on - strand, within Pf1N1B4_2841 at 3186.023 kb on - strand, within Pf1N1B4_2841 at 3186.023 kb on - strand, within Pf1N1B4_2841 at 3186.023 kb on - strand, within Pf1N1B4_2841 at 3186.023 kb on - strand, within Pf1N1B4_2841 at 3186.091 kb on + strand, within Pf1N1B4_2841 at 3186.091 kb on + strand, within Pf1N1B4_2841 at 3186.091 kb on + strand, within Pf1N1B4_2841 at 3186.091 kb on + strand at 3186.091 kb on + strand, within Pf1N1B4_2841 at 3186.106 kb on + strand, within Pf1N1B4_2841 at 3186.106 kb on + strand, within Pf1N1B4_2841 at 3186.107 kb on - strand, within Pf1N1B4_2841 at 3186.107 kb on - strand, within Pf1N1B4_2841 at 3186.166 kb on - strand, within Pf1N1B4_2841 at 3186.240 kb on + strand, within Pf1N1B4_2841 at 3186.324 kb on - strand, within Pf1N1B4_2841 at 3186.372 kb on + strand at 3186.457 kb on + strand at 3186.457 kb on + strand at 3186.457 kb on + strand at 3186.457 kb on + strand at 3186.458 kb on - strand at 3186.458 kb on - strand at 3186.458 kb on - strand at 3186.458 kb on - strand at 3186.458 kb on - strand at 3186.458 kb on - strand at 3186.459 kb on + strand at 3186.459 kb on + strand at 3186.459 kb on + strand at 3186.459 kb on + strand at 3186.459 kb on + strand at 3186.459 kb on + strand at 3186.459 kb on + strand at 3186.459 kb on + strand at 3186.459 kb on + strand at 3186.459 kb on + strand at 3186.460 kb on - strand at 3186.460 kb on - strand at 3186.460 kb on - strand at 3186.460 kb on - strand at 3186.460 kb on - strand at 3186.460 kb on - strand at 3186.460 kb on - strand at 3186.562 kb on + strand at 3186.660 kb on - strand at 3186.669 kb on + strand, within Pf1N1B4_2842 at 3186.669 kb on + strand, within Pf1N1B4_2842 at 3186.669 kb on + strand, within Pf1N1B4_2842 at 3186.669 kb on + strand, within Pf1N1B4_2842 at 3186.669 kb on + strand, within Pf1N1B4_2842 at 3186.670 kb on - strand, within Pf1N1B4_2842 at 3186.670 kb on - strand, within Pf1N1B4_2842 at 3186.670 kb on - strand, within Pf1N1B4_2842 at 3186.690 kb on + strand, within Pf1N1B4_2842 at 3186.690 kb on + strand, within Pf1N1B4_2842 at 3186.690 kb on + strand, within Pf1N1B4_2842 at 3186.726 kb on + strand, within Pf1N1B4_2842 at 3186.726 kb on + strand, within Pf1N1B4_2842 at 3186.726 kb on + strand, within Pf1N1B4_2842 at 3186.726 kb on + strand, within Pf1N1B4_2842 at 3186.726 kb on + strand, within Pf1N1B4_2842 at 3186.726 kb on + strand, within Pf1N1B4_2842 at 3186.726 kb on + strand, within Pf1N1B4_2842 at 3186.726 kb on + strand, within Pf1N1B4_2842 at 3186.726 kb on + strand, within Pf1N1B4_2842 at 3186.727 kb on - strand, within Pf1N1B4_2842 at 3186.727 kb on - strand at 3186.727 kb on - strand, within Pf1N1B4_2842 at 3186.727 kb on - strand, within Pf1N1B4_2842 at 3186.727 kb on - strand, within Pf1N1B4_2842 at 3186.727 kb on - strand, within Pf1N1B4_2842 at 3187.144 kb on + strand, within Pf1N1B4_2842 at 3187.144 kb on + strand, within Pf1N1B4_2842 at 3187.144 kb on + strand, within Pf1N1B4_2842 at 3187.144 kb on + strand, within Pf1N1B4_2842 at 3187.145 kb on - strand, within Pf1N1B4_2842 at 3187.182 kb on + strand, within Pf1N1B4_2842 at 3187.183 kb on - strand at 3187.183 kb on - strand at 3187.183 kb on - strand, within Pf1N1B4_2842 at 3187.183 kb on - strand, within Pf1N1B4_2842 at 3187.260 kb on + strand, within Pf1N1B4_2842 at 3187.260 kb on + strand, within Pf1N1B4_2842 at 3187.260 kb on + strand, within Pf1N1B4_2842 at 3187.260 kb on + strand, within Pf1N1B4_2842 at 3187.260 kb on + strand, within Pf1N1B4_2842 at 3187.260 kb on + strand at 3187.260 kb on + strand, within Pf1N1B4_2842 at 3187.260 kb on + strand, within Pf1N1B4_2842 at 3187.260 kb on + strand, within Pf1N1B4_2842 at 3187.261 kb on - strand, within Pf1N1B4_2842 at 3187.261 kb on - strand, within Pf1N1B4_2842 at 3187.261 kb on - strand, within Pf1N1B4_2842 at 3187.261 kb on - strand, within Pf1N1B4_2842 at 3187.261 kb on - strand, within Pf1N1B4_2842 at 3187.261 kb on - strand, within Pf1N1B4_2842 at 3187.261 kb on - strand, within Pf1N1B4_2842 at 3187.261 kb on - strand, within Pf1N1B4_2842 at 3187.261 kb on - strand, within Pf1N1B4_2842 at 3187.261 kb on - strand, within Pf1N1B4_2842 at 3187.261 kb on - strand, within Pf1N1B4_2842 at 3187.334 kb on + strand, within Pf1N1B4_2842 at 3187.334 kb on + strand, within Pf1N1B4_2842 at 3187.334 kb on + strand, within Pf1N1B4_2842 at 3187.334 kb on + strand at 3187.334 kb on + strand, within Pf1N1B4_2842 at 3187.337 kb on + strand, within Pf1N1B4_2842 at 3187.338 kb on - strand, within Pf1N1B4_2842
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 300 mM remove 3,184,395 - -0.2 3,184,423 - -0.8 3,184,423 - -0.8 3,184,423 - -0.0 3,184,423 - +0.2 3,184,423 - +0.3 3,184,434 - +0.8 3,184,434 - -0.4 3,184,480 - +0.2 3,184,673 + Pf1N1B4_2840 0.32 -0.4 3,185,001 + Pf1N1B4_2840 0.69 +0.2 3,185,001 + Pf1N1B4_2840 0.69 -0.8 3,185,002 - +1.2 3,185,002 - Pf1N1B4_2840 0.69 -1.6 3,185,002 - Pf1N1B4_2840 0.69 +1.3 3,185,002 - Pf1N1B4_2840 0.69 +1.2 3,185,002 - Pf1N1B4_2840 0.69 +0.5 3,185,073 - Pf1N1B4_2840 0.77 +1.2 3,185,082 + Pf1N1B4_2840 0.78 +0.6 3,185,082 + Pf1N1B4_2840 0.78 +0.3 3,185,082 + Pf1N1B4_2840 0.78 -0.4 3,185,083 - Pf1N1B4_2840 0.79 -0.4 3,185,083 - Pf1N1B4_2840 0.79 +0.4 3,185,083 - Pf1N1B4_2840 0.79 +0.2 3,185,083 - Pf1N1B4_2840 0.79 -1.3 3,185,083 - Pf1N1B4_2840 0.79 +0.2 3,185,269 + +0.8 3,185,269 + -0.4 3,185,270 - -0.4 3,185,270 - -1.0 3,185,270 - -1.4 3,185,270 - -1.7 3,185,270 - +0.8 3,185,270 - -2.6 3,185,305 + -1.2 3,185,305 + -1.4 3,185,306 - -1.8 3,185,343 + -0.0 3,185,344 - -0.8 3,185,346 + -0.6 3,185,346 + +0.7 3,185,347 - +0.2 3,185,347 - -0.4 3,185,410 + Pf1N1B4_2841 0.11 -1.0 3,185,410 + Pf1N1B4_2841 0.11 +0.5 3,185,411 - Pf1N1B4_2841 0.11 +0.2 3,185,411 - Pf1N1B4_2841 0.11 -0.1 3,185,496 + Pf1N1B4_2841 0.18 -1.2 3,185,496 + Pf1N1B4_2841 0.18 -0.6 3,185,496 + -0.8 3,185,496 + Pf1N1B4_2841 0.18 +0.2 3,185,497 - Pf1N1B4_2841 0.19 +0.4 3,185,497 - Pf1N1B4_2841 0.19 -1.9 3,185,497 - Pf1N1B4_2841 0.19 -1.2 3,185,497 - Pf1N1B4_2841 0.19 -1.2 3,185,497 - Pf1N1B4_2841 0.19 -0.4 3,185,497 - Pf1N1B4_2841 0.19 +1.3 3,185,498 + Pf1N1B4_2841 0.19 -0.1 3,185,498 + Pf1N1B4_2841 0.19 -0.8 3,185,553 + Pf1N1B4_2841 0.23 -1.2 3,185,553 + Pf1N1B4_2841 0.23 +0.5 3,185,559 + Pf1N1B4_2841 0.24 -0.3 3,185,559 + Pf1N1B4_2841 0.24 +0.8 3,185,559 + Pf1N1B4_2841 0.24 +0.3 3,185,559 + +2.8 3,185,562 + Pf1N1B4_2841 0.24 +2.8 3,185,562 + Pf1N1B4_2841 0.24 +1.2 3,185,563 - Pf1N1B4_2841 0.24 +0.2 3,185,686 + Pf1N1B4_2841 0.34 +2.0 3,185,686 + Pf1N1B4_2841 0.34 +0.2 3,185,687 - Pf1N1B4_2841 0.34 -0.7 3,185,687 - +1.2 3,185,687 - Pf1N1B4_2841 0.34 +1.5 3,185,971 + Pf1N1B4_2841 0.57 +0.2 3,185,971 + Pf1N1B4_2841 0.57 +1.7 3,185,972 - Pf1N1B4_2841 0.58 -2.4 3,186,022 + Pf1N1B4_2841 0.62 +0.8 3,186,022 + Pf1N1B4_2841 0.62 -0.1 3,186,022 + Pf1N1B4_2841 0.62 +0.5 3,186,022 + Pf1N1B4_2841 0.62 -0.5 3,186,023 - Pf1N1B4_2841 0.62 +1.2 3,186,023 - Pf1N1B4_2841 0.62 -1.2 3,186,023 - Pf1N1B4_2841 0.62 +0.7 3,186,023 - Pf1N1B4_2841 0.62 +0.6 3,186,023 - Pf1N1B4_2841 0.62 +0.5 3,186,091 + Pf1N1B4_2841 0.67 -0.4 3,186,091 + Pf1N1B4_2841 0.67 -0.4 3,186,091 + Pf1N1B4_2841 0.67 +1.3 3,186,091 + +2.8 3,186,091 + Pf1N1B4_2841 0.67 -2.0 3,186,106 + Pf1N1B4_2841 0.69 -0.4 3,186,106 + Pf1N1B4_2841 0.69 +0.4 3,186,107 - Pf1N1B4_2841 0.69 -0.5 3,186,107 - Pf1N1B4_2841 0.69 -0.4 3,186,166 - Pf1N1B4_2841 0.73 +0.5 3,186,240 + Pf1N1B4_2841 0.80 -0.2 3,186,324 - Pf1N1B4_2841 0.86 +1.4 3,186,372 + -1.4 3,186,457 + -0.5 3,186,457 + +0.8 3,186,457 + -0.3 3,186,457 + -1.2 3,186,458 - -0.1 3,186,458 - -0.2 3,186,458 - -1.4 3,186,458 - +1.2 3,186,458 - -0.8 3,186,458 - -0.6 3,186,459 + -1.6 3,186,459 + -0.6 3,186,459 + +0.2 3,186,459 + +0.6 3,186,459 + +0.2 3,186,459 + +1.2 3,186,459 + -0.8 3,186,459 + -2.0 3,186,459 + +0.5 3,186,459 + +0.6 3,186,460 - +0.6 3,186,460 - -0.0 3,186,460 - +1.8 3,186,460 - -1.2 3,186,460 - -0.1 3,186,460 - +0.3 3,186,460 - -1.2 3,186,562 + -0.1 3,186,660 - -1.2 3,186,669 + Pf1N1B4_2842 0.10 +1.2 3,186,669 + Pf1N1B4_2842 0.10 -0.1 3,186,669 + Pf1N1B4_2842 0.10 -0.4 3,186,669 + Pf1N1B4_2842 0.10 -1.1 3,186,669 + Pf1N1B4_2842 0.10 -0.1 3,186,670 - Pf1N1B4_2842 0.11 -0.5 3,186,670 - Pf1N1B4_2842 0.11 -2.2 3,186,670 - Pf1N1B4_2842 0.11 -0.5 3,186,690 + Pf1N1B4_2842 0.12 +0.2 3,186,690 + Pf1N1B4_2842 0.12 +3.2 3,186,690 + Pf1N1B4_2842 0.12 -2.0 3,186,726 + Pf1N1B4_2842 0.14 -1.8 3,186,726 + Pf1N1B4_2842 0.14 -0.1 3,186,726 + Pf1N1B4_2842 0.14 +1.2 3,186,726 + Pf1N1B4_2842 0.14 +0.8 3,186,726 + Pf1N1B4_2842 0.14 +1.2 3,186,726 + Pf1N1B4_2842 0.14 +0.2 3,186,726 + Pf1N1B4_2842 0.14 +3.5 3,186,726 + Pf1N1B4_2842 0.14 +0.8 3,186,726 + Pf1N1B4_2842 0.14 -1.2 3,186,727 - Pf1N1B4_2842 0.14 +0.4 3,186,727 - -1.2 3,186,727 - Pf1N1B4_2842 0.14 +1.2 3,186,727 - Pf1N1B4_2842 0.14 -0.8 3,186,727 - Pf1N1B4_2842 0.14 +0.3 3,186,727 - Pf1N1B4_2842 0.14 -0.4 3,187,144 + Pf1N1B4_2842 0.38 +0.2 3,187,144 + Pf1N1B4_2842 0.38 -1.7 3,187,144 + Pf1N1B4_2842 0.38 +0.8 3,187,144 + Pf1N1B4_2842 0.38 -0.8 3,187,145 - Pf1N1B4_2842 0.38 -0.6 3,187,182 + Pf1N1B4_2842 0.41 -0.6 3,187,183 - +0.6 3,187,183 - +1.2 3,187,183 - Pf1N1B4_2842 0.41 +0.2 3,187,183 - Pf1N1B4_2842 0.41 -1.4 3,187,260 + Pf1N1B4_2842 0.45 +0.8 3,187,260 + Pf1N1B4_2842 0.45 +0.4 3,187,260 + Pf1N1B4_2842 0.45 +1.6 3,187,260 + Pf1N1B4_2842 0.45 -0.5 3,187,260 + Pf1N1B4_2842 0.45 -1.6 3,187,260 + -1.4 3,187,260 + Pf1N1B4_2842 0.45 +1.2 3,187,260 + Pf1N1B4_2842 0.45 +1.2 3,187,260 + Pf1N1B4_2842 0.45 +0.0 3,187,261 - Pf1N1B4_2842 0.45 -1.6 3,187,261 - Pf1N1B4_2842 0.45 -0.3 3,187,261 - Pf1N1B4_2842 0.45 +0.4 3,187,261 - Pf1N1B4_2842 0.45 +0.6 3,187,261 - Pf1N1B4_2842 0.45 -0.6 3,187,261 - Pf1N1B4_2842 0.45 -0.0 3,187,261 - Pf1N1B4_2842 0.45 -0.1 3,187,261 - Pf1N1B4_2842 0.45 -0.3 3,187,261 - Pf1N1B4_2842 0.45 -0.3 3,187,261 - Pf1N1B4_2842 0.45 +0.2 3,187,261 - Pf1N1B4_2842 0.45 -0.4 3,187,334 + Pf1N1B4_2842 0.50 -1.2 3,187,334 + Pf1N1B4_2842 0.50 -0.7 3,187,334 + Pf1N1B4_2842 0.50 +0.2 3,187,334 + +1.2 3,187,334 + Pf1N1B4_2842 0.50 -0.8 3,187,337 + Pf1N1B4_2842 0.50 -0.2 3,187,338 - Pf1N1B4_2842 0.50 -0.8
Or see this region's nucleotide sequence