Experiment: Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2633 and Pf1N1B4_2634 are separated by 453 nucleotides Pf1N1B4_2634 and Pf1N1B4_2635 are separated by 69 nucleotides Pf1N1B4_2635 and Pf1N1B4_2636 are separated by 175 nucleotides Pf1N1B4_2636 and Pf1N1B4_2637 are separated by 32 nucleotides
Pf1N1B4_2633 - Putative drug efflux protein, at 2,970,973 to 2,972,184
_2633
Pf1N1B4_2634 - Lhr-like helicases, at 2,972,638 to 2,976,840
_2634
Pf1N1B4_2635 - hypothetical protein, at 2,976,910 to 2,977,119
_2635
Pf1N1B4_2636 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 2,977,295 to 2,977,411
_2636
Pf1N1B4_2637 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 2,977,444 to 2,978,163
_2637
Position (kb)
2972
2973
2974
2975
2976
2977 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2971.655 kb on + strand, within Pf1N1B4_2633 at 2971.655 kb on + strand, within Pf1N1B4_2633 at 2971.655 kb on + strand, within Pf1N1B4_2633 at 2971.656 kb on - strand, within Pf1N1B4_2633 at 2971.656 kb on - strand, within Pf1N1B4_2633 at 2971.656 kb on - strand, within Pf1N1B4_2633 at 2971.656 kb on - strand, within Pf1N1B4_2633 at 2971.913 kb on + strand, within Pf1N1B4_2633 at 2971.913 kb on + strand, within Pf1N1B4_2633 at 2971.913 kb on + strand, within Pf1N1B4_2633 at 2971.914 kb on - strand, within Pf1N1B4_2633 at 2972.045 kb on + strand, within Pf1N1B4_2633 at 2972.046 kb on - strand, within Pf1N1B4_2633 at 2972.046 kb on - strand, within Pf1N1B4_2633 at 2972.103 kb on + strand at 2972.103 kb on + strand at 2972.103 kb on + strand at 2972.103 kb on + strand at 2972.103 kb on + strand at 2972.103 kb on + strand at 2972.103 kb on + strand at 2972.103 kb on + strand at 2972.104 kb on - strand at 2972.104 kb on - strand at 2972.104 kb on - strand at 2972.104 kb on - strand at 2972.104 kb on - strand at 2972.108 kb on - strand at 2972.108 kb on - strand at 2972.108 kb on - strand at 2972.108 kb on - strand at 2972.108 kb on - strand at 2972.248 kb on + strand at 2972.249 kb on - strand at 2972.350 kb on + strand at 2972.443 kb on + strand at 2972.443 kb on + strand at 2972.460 kb on + strand at 2972.460 kb on + strand at 2972.460 kb on + strand at 2972.460 kb on + strand at 2972.460 kb on + strand at 2972.461 kb on - strand at 2972.461 kb on - strand at 2972.461 kb on - strand at 2972.461 kb on - strand at 2972.461 kb on - strand at 2972.463 kb on + strand at 2972.463 kb on + strand at 2972.463 kb on + strand at 2972.463 kb on + strand at 2972.464 kb on - strand at 2972.464 kb on - strand at 2972.573 kb on + strand at 2972.748 kb on - strand at 2973.115 kb on - strand, within Pf1N1B4_2634 at 2973.478 kb on + strand, within Pf1N1B4_2634 at 2973.478 kb on + strand, within Pf1N1B4_2634 at 2973.478 kb on + strand, within Pf1N1B4_2634 at 2973.479 kb on - strand, within Pf1N1B4_2634 at 2973.479 kb on - strand, within Pf1N1B4_2634 at 2973.479 kb on - strand, within Pf1N1B4_2634 at 2973.479 kb on - strand, within Pf1N1B4_2634 at 2973.562 kb on + strand, within Pf1N1B4_2634 at 2973.562 kb on + strand, within Pf1N1B4_2634 at 2973.562 kb on + strand at 2973.562 kb on + strand, within Pf1N1B4_2634 at 2973.563 kb on - strand, within Pf1N1B4_2634 at 2973.563 kb on - strand, within Pf1N1B4_2634 at 2973.563 kb on - strand, within Pf1N1B4_2634 at 2973.588 kb on - strand, within Pf1N1B4_2634 at 2973.588 kb on - strand, within Pf1N1B4_2634 at 2973.588 kb on - strand, within Pf1N1B4_2634 at 2973.588 kb on - strand, within Pf1N1B4_2634 at 2973.791 kb on + strand, within Pf1N1B4_2634 at 2973.872 kb on + strand, within Pf1N1B4_2634 at 2973.872 kb on + strand, within Pf1N1B4_2634 at 2973.872 kb on + strand, within Pf1N1B4_2634 at 2973.872 kb on + strand, within Pf1N1B4_2634 at 2973.872 kb on + strand, within Pf1N1B4_2634 at 2973.872 kb on + strand, within Pf1N1B4_2634 at 2973.873 kb on - strand, within Pf1N1B4_2634 at 2973.873 kb on - strand, within Pf1N1B4_2634 at 2973.873 kb on - strand, within Pf1N1B4_2634 at 2973.873 kb on - strand, within Pf1N1B4_2634 at 2973.923 kb on - strand, within Pf1N1B4_2634 at 2974.073 kb on + strand, within Pf1N1B4_2634 at 2974.416 kb on - strand, within Pf1N1B4_2634 at 2974.416 kb on - strand, within Pf1N1B4_2634 at 2974.688 kb on - strand, within Pf1N1B4_2634 at 2974.688 kb on - strand, within Pf1N1B4_2634 at 2974.707 kb on - strand, within Pf1N1B4_2634 at 2974.791 kb on + strand, within Pf1N1B4_2634 at 2974.791 kb on + strand, within Pf1N1B4_2634 at 2974.791 kb on + strand, within Pf1N1B4_2634 at 2974.791 kb on + strand, within Pf1N1B4_2634 at 2974.791 kb on + strand, within Pf1N1B4_2634 at 2974.791 kb on + strand, within Pf1N1B4_2634 at 2974.792 kb on - strand, within Pf1N1B4_2634 at 2974.792 kb on - strand, within Pf1N1B4_2634 at 2974.792 kb on - strand, within Pf1N1B4_2634 at 2974.792 kb on - strand, within Pf1N1B4_2634 at 2975.048 kb on - strand, within Pf1N1B4_2634 at 2975.048 kb on - strand, within Pf1N1B4_2634 at 2975.081 kb on + strand, within Pf1N1B4_2634 at 2975.122 kb on + strand, within Pf1N1B4_2634 at 2975.123 kb on - strand, within Pf1N1B4_2634 at 2975.327 kb on + strand, within Pf1N1B4_2634 at 2975.334 kb on + strand, within Pf1N1B4_2634 at 2975.334 kb on + strand, within Pf1N1B4_2634 at 2975.335 kb on - strand, within Pf1N1B4_2634 at 2975.335 kb on - strand, within Pf1N1B4_2634 at 2975.335 kb on - strand, within Pf1N1B4_2634 at 2975.364 kb on + strand, within Pf1N1B4_2634 at 2975.364 kb on + strand, within Pf1N1B4_2634 at 2975.432 kb on + strand, within Pf1N1B4_2634 at 2975.432 kb on + strand, within Pf1N1B4_2634 at 2975.532 kb on - strand, within Pf1N1B4_2634 at 2975.562 kb on - strand, within Pf1N1B4_2634 at 2975.562 kb on - strand, within Pf1N1B4_2634 at 2976.156 kb on - strand, within Pf1N1B4_2634 at 2976.156 kb on - strand, within Pf1N1B4_2634 at 2976.156 kb on - strand, within Pf1N1B4_2634 at 2976.156 kb on - strand, within Pf1N1B4_2634 at 2976.156 kb on - strand, within Pf1N1B4_2634 at 2976.156 kb on - strand, within Pf1N1B4_2634 at 2976.214 kb on + strand, within Pf1N1B4_2634 at 2976.214 kb on + strand, within Pf1N1B4_2634 at 2976.214 kb on + strand, within Pf1N1B4_2634 at 2976.214 kb on + strand, within Pf1N1B4_2634 at 2976.214 kb on + strand at 2976.214 kb on + strand, within Pf1N1B4_2634 at 2976.214 kb on + strand, within Pf1N1B4_2634 at 2976.214 kb on + strand, within Pf1N1B4_2634 at 2976.214 kb on + strand, within Pf1N1B4_2634 at 2976.214 kb on + strand, within Pf1N1B4_2634 at 2976.214 kb on + strand, within Pf1N1B4_2634 at 2976.214 kb on + strand, within Pf1N1B4_2634 at 2976.214 kb on + strand, within Pf1N1B4_2634 at 2976.215 kb on - strand, within Pf1N1B4_2634 at 2976.215 kb on - strand at 2976.215 kb on - strand, within Pf1N1B4_2634 at 2976.215 kb on - strand, within Pf1N1B4_2634 at 2976.215 kb on - strand, within Pf1N1B4_2634 at 2976.215 kb on - strand, within Pf1N1B4_2634 at 2976.215 kb on - strand, within Pf1N1B4_2634 at 2976.215 kb on - strand, within Pf1N1B4_2634 at 2976.215 kb on - strand, within Pf1N1B4_2634 at 2976.215 kb on - strand, within Pf1N1B4_2634 at 2976.215 kb on - strand, within Pf1N1B4_2634 at 2976.215 kb on - strand, within Pf1N1B4_2634 at 2976.215 kb on - strand, within Pf1N1B4_2634 at 2976.488 kb on + strand at 2976.488 kb on + strand at 2976.489 kb on - strand at 2976.489 kb on - strand at 2976.489 kb on - strand at 2976.489 kb on - strand at 2976.489 kb on - strand at 2976.882 kb on + strand at 2976.965 kb on - strand, within Pf1N1B4_2635 at 2976.965 kb on - strand, within Pf1N1B4_2635 at 2976.965 kb on - strand, within Pf1N1B4_2635 at 2976.965 kb on - strand, within Pf1N1B4_2635 at 2976.992 kb on + strand, within Pf1N1B4_2635 at 2977.073 kb on - strand, within Pf1N1B4_2635 at 2977.073 kb on - strand, within Pf1N1B4_2635 at 2977.127 kb on - strand at 2977.152 kb on + strand at 2977.153 kb on - strand at 2977.153 kb on - strand at 2977.153 kb on - strand at 2977.153 kb on - strand at 2977.153 kb on - strand at 2977.153 kb on - strand at 2977.153 kb on - strand at 2977.153 kb on - strand at 2977.153 kb on - strand at 2977.160 kb on - strand at 2977.210 kb on + strand at 2977.279 kb on + strand at 2977.279 kb on + strand at 2977.279 kb on + strand at 2977.279 kb on + strand at 2977.279 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.294 kb on + strand at 2977.299 kb on + strand at 2977.299 kb on + strand at 2977.299 kb on + strand at 2977.459 kb on + strand at 2977.459 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.462 kb on + strand at 2977.585 kb on + strand, within Pf1N1B4_2637 at 2977.618 kb on + strand, within Pf1N1B4_2637 at 2977.618 kb on + strand, within Pf1N1B4_2637
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 300 mM remove 2,971,655 + Pf1N1B4_2633 0.56 +1.2 2,971,655 + Pf1N1B4_2633 0.56 +1.7 2,971,655 + Pf1N1B4_2633 0.56 +0.2 2,971,656 - Pf1N1B4_2633 0.56 -0.2 2,971,656 - Pf1N1B4_2633 0.56 +0.3 2,971,656 - Pf1N1B4_2633 0.56 +0.2 2,971,656 - Pf1N1B4_2633 0.56 +0.6 2,971,913 + Pf1N1B4_2633 0.78 -0.8 2,971,913 + Pf1N1B4_2633 0.78 -0.1 2,971,913 + Pf1N1B4_2633 0.78 -0.1 2,971,914 - Pf1N1B4_2633 0.78 -0.4 2,972,045 + Pf1N1B4_2633 0.88 +0.6 2,972,046 - Pf1N1B4_2633 0.89 -1.2 2,972,046 - Pf1N1B4_2633 0.89 -2.0 2,972,103 + +0.4 2,972,103 + +0.0 2,972,103 + -2.4 2,972,103 + +0.2 2,972,103 + +0.6 2,972,103 + +0.2 2,972,103 + +0.3 2,972,103 + +0.2 2,972,104 - +0.6 2,972,104 - -0.8 2,972,104 - -1.4 2,972,104 - +0.3 2,972,104 - -1.8 2,972,108 - +0.6 2,972,108 - +1.9 2,972,108 - -1.4 2,972,108 - -0.0 2,972,108 - -0.8 2,972,248 + -2.0 2,972,249 - -0.4 2,972,350 + -0.6 2,972,443 + +1.4 2,972,443 + +0.4 2,972,460 + -0.4 2,972,460 + +0.2 2,972,460 + -0.9 2,972,460 + +0.8 2,972,460 + +1.2 2,972,461 - +0.6 2,972,461 - +1.0 2,972,461 - -1.4 2,972,461 - -2.0 2,972,461 - +0.4 2,972,463 + -0.1 2,972,463 + +0.2 2,972,463 + -0.4 2,972,463 + +0.5 2,972,464 - +0.2 2,972,464 - -1.6 2,972,573 + -1.6 2,972,748 - +1.0 2,973,115 - Pf1N1B4_2634 0.11 +2.2 2,973,478 + Pf1N1B4_2634 0.20 +0.8 2,973,478 + Pf1N1B4_2634 0.20 -0.0 2,973,478 + Pf1N1B4_2634 0.20 +1.5 2,973,479 - Pf1N1B4_2634 0.20 +1.6 2,973,479 - Pf1N1B4_2634 0.20 +0.2 2,973,479 - Pf1N1B4_2634 0.20 +0.0 2,973,479 - Pf1N1B4_2634 0.20 -0.4 2,973,562 + Pf1N1B4_2634 0.22 +0.6 2,973,562 + Pf1N1B4_2634 0.22 +2.6 2,973,562 + -0.4 2,973,562 + Pf1N1B4_2634 0.22 -0.8 2,973,563 - Pf1N1B4_2634 0.22 -1.8 2,973,563 - Pf1N1B4_2634 0.22 +0.2 2,973,563 - Pf1N1B4_2634 0.22 -1.6 2,973,588 - Pf1N1B4_2634 0.23 +0.2 2,973,588 - Pf1N1B4_2634 0.23 +1.5 2,973,588 - Pf1N1B4_2634 0.23 +0.2 2,973,588 - Pf1N1B4_2634 0.23 -0.5 2,973,791 + Pf1N1B4_2634 0.27 -1.1 2,973,872 + Pf1N1B4_2634 0.29 +2.0 2,973,872 + Pf1N1B4_2634 0.29 +0.2 2,973,872 + Pf1N1B4_2634 0.29 -0.0 2,973,872 + Pf1N1B4_2634 0.29 -1.0 2,973,872 + Pf1N1B4_2634 0.29 -1.1 2,973,872 + Pf1N1B4_2634 0.29 -1.0 2,973,873 - Pf1N1B4_2634 0.29 -1.0 2,973,873 - Pf1N1B4_2634 0.29 -1.1 2,973,873 - Pf1N1B4_2634 0.29 -1.0 2,973,873 - Pf1N1B4_2634 0.29 -0.1 2,973,923 - Pf1N1B4_2634 0.31 -2.0 2,974,073 + Pf1N1B4_2634 0.34 -1.2 2,974,416 - Pf1N1B4_2634 0.42 -0.2 2,974,416 - Pf1N1B4_2634 0.42 +0.0 2,974,688 - Pf1N1B4_2634 0.49 +0.4 2,974,688 - Pf1N1B4_2634 0.49 -0.1 2,974,707 - Pf1N1B4_2634 0.49 -0.2 2,974,791 + Pf1N1B4_2634 0.51 -1.1 2,974,791 + Pf1N1B4_2634 0.51 +0.5 2,974,791 + Pf1N1B4_2634 0.51 +1.0 2,974,791 + Pf1N1B4_2634 0.51 -0.6 2,974,791 + Pf1N1B4_2634 0.51 +1.2 2,974,791 + Pf1N1B4_2634 0.51 +0.6 2,974,792 - Pf1N1B4_2634 0.51 +1.1 2,974,792 - Pf1N1B4_2634 0.51 +0.5 2,974,792 - Pf1N1B4_2634 0.51 -0.3 2,974,792 - Pf1N1B4_2634 0.51 +0.5 2,975,048 - Pf1N1B4_2634 0.57 -0.4 2,975,048 - Pf1N1B4_2634 0.57 -0.0 2,975,081 + Pf1N1B4_2634 0.58 -0.8 2,975,122 + Pf1N1B4_2634 0.59 +1.2 2,975,123 - Pf1N1B4_2634 0.59 +3.2 2,975,327 + Pf1N1B4_2634 0.64 +2.5 2,975,334 + Pf1N1B4_2634 0.64 -0.2 2,975,334 + Pf1N1B4_2634 0.64 -1.1 2,975,335 - Pf1N1B4_2634 0.64 +0.2 2,975,335 - Pf1N1B4_2634 0.64 +1.0 2,975,335 - Pf1N1B4_2634 0.64 +0.2 2,975,364 + Pf1N1B4_2634 0.65 -1.6 2,975,364 + Pf1N1B4_2634 0.65 -0.4 2,975,432 + Pf1N1B4_2634 0.66 -1.8 2,975,432 + Pf1N1B4_2634 0.66 -1.4 2,975,532 - Pf1N1B4_2634 0.69 +0.0 2,975,562 - Pf1N1B4_2634 0.70 -2.0 2,975,562 - Pf1N1B4_2634 0.70 -1.8 2,976,156 - Pf1N1B4_2634 0.84 +0.0 2,976,156 - Pf1N1B4_2634 0.84 +2.2 2,976,156 - Pf1N1B4_2634 0.84 -0.4 2,976,156 - Pf1N1B4_2634 0.84 -1.0 2,976,156 - Pf1N1B4_2634 0.84 +1.8 2,976,156 - Pf1N1B4_2634 0.84 +1.8 2,976,214 + Pf1N1B4_2634 0.85 -2.2 2,976,214 + Pf1N1B4_2634 0.85 -0.6 2,976,214 + Pf1N1B4_2634 0.85 -0.6 2,976,214 + Pf1N1B4_2634 0.85 -0.9 2,976,214 + +1.2 2,976,214 + Pf1N1B4_2634 0.85 +0.3 2,976,214 + Pf1N1B4_2634 0.85 -0.4 2,976,214 + Pf1N1B4_2634 0.85 -1.1 2,976,214 + Pf1N1B4_2634 0.85 -0.8 2,976,214 + Pf1N1B4_2634 0.85 -0.4 2,976,214 + Pf1N1B4_2634 0.85 +0.1 2,976,214 + Pf1N1B4_2634 0.85 -0.6 2,976,214 + Pf1N1B4_2634 0.85 +0.6 2,976,215 - Pf1N1B4_2634 0.85 -1.4 2,976,215 - -0.8 2,976,215 - Pf1N1B4_2634 0.85 -2.1 2,976,215 - Pf1N1B4_2634 0.85 -0.2 2,976,215 - Pf1N1B4_2634 0.85 -0.8 2,976,215 - Pf1N1B4_2634 0.85 +1.2 2,976,215 - Pf1N1B4_2634 0.85 +1.6 2,976,215 - Pf1N1B4_2634 0.85 -0.8 2,976,215 - Pf1N1B4_2634 0.85 +0.6 2,976,215 - Pf1N1B4_2634 0.85 -0.1 2,976,215 - Pf1N1B4_2634 0.85 +0.6 2,976,215 - Pf1N1B4_2634 0.85 -0.2 2,976,215 - Pf1N1B4_2634 0.85 -0.9 2,976,488 + +0.8 2,976,488 + +0.2 2,976,489 - -0.1 2,976,489 - +1.6 2,976,489 - +0.2 2,976,489 - -0.8 2,976,489 - -0.2 2,976,882 + +0.6 2,976,965 - Pf1N1B4_2635 0.26 +0.8 2,976,965 - Pf1N1B4_2635 0.26 +0.6 2,976,965 - Pf1N1B4_2635 0.26 +0.2 2,976,965 - Pf1N1B4_2635 0.26 +1.5 2,976,992 + Pf1N1B4_2635 0.39 +1.2 2,977,073 - Pf1N1B4_2635 0.78 -0.6 2,977,073 - Pf1N1B4_2635 0.78 +0.5 2,977,127 - +0.4 2,977,152 + -1.0 2,977,153 - -0.6 2,977,153 - +1.8 2,977,153 - -0.4 2,977,153 - +0.8 2,977,153 - +0.8 2,977,153 - +0.4 2,977,153 - +0.1 2,977,153 - +0.1 2,977,153 - +0.4 2,977,160 - +0.2 2,977,210 + +0.2 2,977,279 + +0.0 2,977,279 + +0.9 2,977,279 + -2.1 2,977,279 + +0.3 2,977,279 + -1.2 2,977,294 + -0.5 2,977,294 + +0.1 2,977,294 + +0.4 2,977,294 + -1.8 2,977,294 + +0.8 2,977,294 + +0.5 2,977,294 + +1.0 2,977,294 + -0.4 2,977,294 + +0.7 2,977,294 + -0.5 2,977,294 + -0.2 2,977,294 + +0.7 2,977,294 + +0.9 2,977,294 + -0.6 2,977,294 + -1.0 2,977,294 + +1.0 2,977,294 + +0.8 2,977,294 + +0.2 2,977,294 + +0.2 2,977,294 + -2.5 2,977,294 + +0.3 2,977,294 + +1.5 2,977,294 + +1.5 2,977,294 + +0.5 2,977,294 + -0.5 2,977,294 + -1.1 2,977,294 + +0.8 2,977,294 + +0.4 2,977,294 + -0.4 2,977,294 + +0.2 2,977,299 + -0.5 2,977,299 + -0.1 2,977,299 + +1.0 2,977,459 + +0.2 2,977,459 + +0.8 2,977,462 + +0.2 2,977,462 + -0.6 2,977,462 + -0.2 2,977,462 + +0.7 2,977,462 + +2.0 2,977,462 + -1.1 2,977,462 + +0.9 2,977,462 + +1.8 2,977,462 + +0.6 2,977,462 + +0.7 2,977,462 + +0.6 2,977,462 + +0.3 2,977,462 + +0.4 2,977,462 + +1.1 2,977,462 + -1.0 2,977,462 + -1.6 2,977,462 + +0.2 2,977,462 + +0.5 2,977,585 + Pf1N1B4_2637 0.20 -1.4 2,977,618 + Pf1N1B4_2637 0.24 +0.6 2,977,618 + Pf1N1B4_2637 0.24 -1.1
Or see this region's nucleotide sequence