Experiment: Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2415 and Pf1N1B4_2416 are separated by 281 nucleotides Pf1N1B4_2416 and Pf1N1B4_2417 are separated by 170 nucleotides
Pf1N1B4_2415 - TldD family protein, Beta/Gamma-proteobacterial subgroup, at 2,736,823 to 2,738,265
_2415
Pf1N1B4_2416 - Choline dehydrogenase (EC 1.1.99.1), at 2,738,547 to 2,740,250
_2416
Pf1N1B4_2417 - Betaine aldehyde dehydrogenase (EC 1.2.1.8), at 2,740,421 to 2,741,893
_2417
Position (kb)
2738
2739
2740
2741 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2737.725 kb on - strand, within Pf1N1B4_2415 at 2737.725 kb on - strand, within Pf1N1B4_2415 at 2737.796 kb on + strand, within Pf1N1B4_2415 at 2737.892 kb on + strand, within Pf1N1B4_2415 at 2737.892 kb on + strand, within Pf1N1B4_2415 at 2737.892 kb on + strand, within Pf1N1B4_2415 at 2737.892 kb on + strand, within Pf1N1B4_2415 at 2737.940 kb on + strand, within Pf1N1B4_2415 at 2737.940 kb on + strand, within Pf1N1B4_2415 at 2737.940 kb on + strand, within Pf1N1B4_2415 at 2738.075 kb on + strand, within Pf1N1B4_2415 at 2738.189 kb on + strand at 2738.297 kb on + strand at 2738.297 kb on + strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.335 kb on + strand at 2738.340 kb on + strand at 2738.340 kb on + strand at 2738.341 kb on - strand at 2738.358 kb on - strand at 2738.378 kb on + strand at 2738.540 kb on - strand at 2738.540 kb on - strand at 2738.540 kb on - strand at 2738.548 kb on + strand at 2738.611 kb on + strand at 2738.612 kb on - strand at 2738.612 kb on - strand at 2738.612 kb on - strand at 2738.612 kb on - strand at 2738.612 kb on - strand at 2738.614 kb on + strand at 2738.614 kb on + strand at 2738.615 kb on - strand at 2738.615 kb on - strand at 2739.004 kb on + strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.133 kb on + strand, within Pf1N1B4_2416 at 2739.133 kb on + strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.138 kb on - strand, within Pf1N1B4_2416 at 2739.138 kb on - strand, within Pf1N1B4_2416 at 2739.329 kb on - strand, within Pf1N1B4_2416 at 2739.329 kb on - strand, within Pf1N1B4_2416 at 2739.329 kb on - strand, within Pf1N1B4_2416 at 2739.329 kb on - strand, within Pf1N1B4_2416 at 2739.337 kb on + strand, within Pf1N1B4_2416 at 2739.337 kb on + strand, within Pf1N1B4_2416 at 2739.536 kb on - strand, within Pf1N1B4_2416 at 2739.875 kb on - strand, within Pf1N1B4_2416 at 2739.958 kb on + strand, within Pf1N1B4_2416 at 2739.958 kb on + strand, within Pf1N1B4_2416 at 2739.958 kb on + strand, within Pf1N1B4_2416 at 2739.959 kb on - strand, within Pf1N1B4_2416 at 2739.959 kb on - strand, within Pf1N1B4_2416 at 2739.959 kb on - strand, within Pf1N1B4_2416 at 2739.959 kb on - strand, within Pf1N1B4_2416 at 2739.959 kb on - strand, within Pf1N1B4_2416 at 2739.959 kb on - strand, within Pf1N1B4_2416 at 2740.055 kb on - strand, within Pf1N1B4_2416 at 2740.231 kb on + strand at 2740.231 kb on + strand at 2740.231 kb on + strand at 2740.232 kb on - strand at 2740.414 kb on + strand at 2740.414 kb on + strand at 2740.414 kb on + strand at 2740.415 kb on - strand at 2740.422 kb on + strand at 2740.423 kb on - strand at 2740.423 kb on - strand at 2740.702 kb on - strand, within Pf1N1B4_2417 at 2740.876 kb on - strand, within Pf1N1B4_2417 at 2741.055 kb on + strand, within Pf1N1B4_2417 at 2741.055 kb on + strand, within Pf1N1B4_2417
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 300 mM remove 2,737,725 - Pf1N1B4_2415 0.63 -0.5 2,737,725 - Pf1N1B4_2415 0.63 +0.3 2,737,796 + Pf1N1B4_2415 0.67 -0.5 2,737,892 + Pf1N1B4_2415 0.74 -0.6 2,737,892 + Pf1N1B4_2415 0.74 +2.0 2,737,892 + Pf1N1B4_2415 0.74 +0.4 2,737,892 + Pf1N1B4_2415 0.74 -1.0 2,737,940 + Pf1N1B4_2415 0.77 +0.7 2,737,940 + Pf1N1B4_2415 0.77 -2.8 2,737,940 + Pf1N1B4_2415 0.77 -0.3 2,738,075 + Pf1N1B4_2415 0.87 -1.1 2,738,189 + -1.0 2,738,297 + -0.5 2,738,297 + +0.3 2,738,298 - +1.3 2,738,298 - +0.3 2,738,298 - +0.3 2,738,298 - -0.1 2,738,298 - -0.2 2,738,298 - +1.3 2,738,335 + +2.3 2,738,340 + -0.1 2,738,340 + -0.8 2,738,341 - +0.2 2,738,358 - +0.3 2,738,378 + -0.8 2,738,540 - -1.3 2,738,540 - -1.5 2,738,540 - -1.7 2,738,548 + +1.6 2,738,611 + +0.3 2,738,612 - -1.0 2,738,612 - -1.3 2,738,612 - -1.3 2,738,612 - -1.0 2,738,612 - -1.6 2,738,614 + -0.7 2,738,614 + -0.3 2,738,615 - -2.0 2,738,615 - -1.7 2,739,004 + Pf1N1B4_2416 0.27 -1.0 2,739,005 - Pf1N1B4_2416 0.27 -2.0 2,739,005 - Pf1N1B4_2416 0.27 -1.8 2,739,005 - Pf1N1B4_2416 0.27 -2.0 2,739,005 - Pf1N1B4_2416 0.27 -0.9 2,739,005 - Pf1N1B4_2416 0.27 -0.1 2,739,133 + Pf1N1B4_2416 0.34 -0.7 2,739,133 + Pf1N1B4_2416 0.34 -2.4 2,739,134 - Pf1N1B4_2416 0.34 -3.9 2,739,134 - Pf1N1B4_2416 0.34 -0.9 2,739,134 - Pf1N1B4_2416 0.34 -1.0 2,739,134 - Pf1N1B4_2416 0.34 -2.0 2,739,134 - Pf1N1B4_2416 0.34 +0.7 2,739,134 - Pf1N1B4_2416 0.34 -0.7 2,739,138 - Pf1N1B4_2416 0.35 -1.3 2,739,138 - Pf1N1B4_2416 0.35 +0.3 2,739,329 - Pf1N1B4_2416 0.46 -2.3 2,739,329 - Pf1N1B4_2416 0.46 -1.4 2,739,329 - Pf1N1B4_2416 0.46 -1.4 2,739,329 - Pf1N1B4_2416 0.46 -2.6 2,739,337 + Pf1N1B4_2416 0.46 -2.6 2,739,337 + Pf1N1B4_2416 0.46 -1.5 2,739,536 - Pf1N1B4_2416 0.58 -2.4 2,739,875 - Pf1N1B4_2416 0.78 +0.3 2,739,958 + Pf1N1B4_2416 0.83 -1.5 2,739,958 + Pf1N1B4_2416 0.83 -0.9 2,739,958 + Pf1N1B4_2416 0.83 -1.5 2,739,959 - Pf1N1B4_2416 0.83 -0.7 2,739,959 - Pf1N1B4_2416 0.83 -2.0 2,739,959 - Pf1N1B4_2416 0.83 -2.0 2,739,959 - Pf1N1B4_2416 0.83 -1.9 2,739,959 - Pf1N1B4_2416 0.83 -2.0 2,739,959 - Pf1N1B4_2416 0.83 -1.0 2,740,055 - Pf1N1B4_2416 0.88 -1.5 2,740,231 + -3.0 2,740,231 + +0.3 2,740,231 + -1.5 2,740,232 - -2.5 2,740,414 + -2.4 2,740,414 + -0.7 2,740,414 + -1.9 2,740,415 - -0.9 2,740,422 + -1.7 2,740,423 - -0.5 2,740,423 - +0.7 2,740,702 - Pf1N1B4_2417 0.19 -1.5 2,740,876 - Pf1N1B4_2417 0.31 -1.9 2,741,055 + Pf1N1B4_2417 0.43 -2.2 2,741,055 + Pf1N1B4_2417 0.43 -0.5
Or see this region's nucleotide sequence