Experiment: Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2311 and Pf1N1B4_2312 are separated by 148 nucleotides Pf1N1B4_2312 and Pf1N1B4_2313 are separated by 115 nucleotides Pf1N1B4_2313 and Pf1N1B4_2314 are separated by 154 nucleotides Pf1N1B4_2314 and Pf1N1B4_2315 are separated by 28 nucleotides
Pf1N1B4_2311 - 4Fe-4S ferredoxin, iron-sulfur binding, at 2,631,681 to 2,631,932
_2311
Pf1N1B4_2312 - Phosphopantetheine adenylyltransferase (EC 2.7.7.3), at 2,632,081 to 2,632,560
_2312
Pf1N1B4_2313 - FIG022869: Oxidoreductase, GMC family, at 2,632,676 to 2,634,271
_2313
Pf1N1B4_2314 - FIG028932: hypothetical protein, at 2,634,426 to 2,634,971
_2314
Pf1N1B4_2315 - Aldehyde dehydrogenase (EC 1.2.1.3); Probable coniferyl aldehyde dehydrogenase (EC 1.2.1.68), at 2,635,000 to 2,636,430
_2315
Position (kb)
2632
2633
2634
2635 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2631.689 kb on + strand at 2631.707 kb on - strand, within Pf1N1B4_2311 at 2631.707 kb on - strand, within Pf1N1B4_2311 at 2631.936 kb on - strand at 2631.969 kb on - strand at 2632.056 kb on - strand at 2632.082 kb on + strand at 2632.082 kb on + strand at 2632.089 kb on + strand at 2632.649 kb on + strand at 2632.649 kb on + strand at 2632.650 kb on - strand at 2632.651 kb on + strand at 2632.651 kb on + strand at 2632.651 kb on + strand at 2632.651 kb on + strand at 2632.652 kb on - strand at 2632.652 kb on - strand at 2632.664 kb on + strand at 2632.665 kb on - strand at 2632.665 kb on - strand at 2632.902 kb on + strand, within Pf1N1B4_2313 at 2633.058 kb on + strand, within Pf1N1B4_2313 at 2633.073 kb on + strand, within Pf1N1B4_2313 at 2633.074 kb on - strand, within Pf1N1B4_2313 at 2633.269 kb on - strand, within Pf1N1B4_2313 at 2633.269 kb on - strand, within Pf1N1B4_2313 at 2633.316 kb on + strand, within Pf1N1B4_2313 at 2633.316 kb on + strand, within Pf1N1B4_2313 at 2633.316 kb on + strand, within Pf1N1B4_2313 at 2633.317 kb on - strand, within Pf1N1B4_2313 at 2633.317 kb on - strand at 2633.317 kb on - strand, within Pf1N1B4_2313 at 2633.317 kb on - strand, within Pf1N1B4_2313 at 2633.317 kb on - strand, within Pf1N1B4_2313 at 2633.317 kb on - strand, within Pf1N1B4_2313 at 2633.338 kb on - strand, within Pf1N1B4_2313 at 2633.381 kb on + strand, within Pf1N1B4_2313 at 2633.480 kb on + strand, within Pf1N1B4_2313 at 2633.480 kb on + strand, within Pf1N1B4_2313 at 2633.481 kb on - strand, within Pf1N1B4_2313 at 2633.481 kb on - strand, within Pf1N1B4_2313 at 2633.481 kb on - strand, within Pf1N1B4_2313 at 2633.484 kb on + strand, within Pf1N1B4_2313 at 2633.484 kb on + strand, within Pf1N1B4_2313 at 2633.484 kb on + strand, within Pf1N1B4_2313 at 2633.485 kb on - strand, within Pf1N1B4_2313 at 2633.503 kb on + strand, within Pf1N1B4_2313 at 2633.504 kb on - strand, within Pf1N1B4_2313 at 2633.607 kb on + strand, within Pf1N1B4_2313 at 2633.607 kb on + strand, within Pf1N1B4_2313 at 2633.607 kb on + strand, within Pf1N1B4_2313 at 2633.607 kb on + strand, within Pf1N1B4_2313 at 2633.986 kb on + strand, within Pf1N1B4_2313 at 2633.986 kb on + strand, within Pf1N1B4_2313 at 2633.986 kb on + strand, within Pf1N1B4_2313 at 2633.986 kb on + strand, within Pf1N1B4_2313 at 2633.986 kb on + strand, within Pf1N1B4_2313 at 2633.986 kb on + strand, within Pf1N1B4_2313 at 2633.987 kb on - strand, within Pf1N1B4_2313 at 2633.987 kb on - strand, within Pf1N1B4_2313 at 2633.987 kb on - strand, within Pf1N1B4_2313 at 2633.987 kb on - strand, within Pf1N1B4_2313 at 2633.987 kb on - strand, within Pf1N1B4_2313 at 2634.027 kb on + strand, within Pf1N1B4_2313 at 2634.027 kb on + strand, within Pf1N1B4_2313 at 2634.028 kb on - strand, within Pf1N1B4_2313 at 2634.039 kb on + strand, within Pf1N1B4_2313 at 2634.039 kb on + strand, within Pf1N1B4_2313 at 2634.040 kb on - strand, within Pf1N1B4_2313 at 2634.040 kb on - strand, within Pf1N1B4_2313 at 2634.040 kb on - strand, within Pf1N1B4_2313 at 2634.104 kb on + strand, within Pf1N1B4_2313 at 2634.104 kb on + strand, within Pf1N1B4_2313 at 2634.104 kb on + strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.105 kb on - strand, within Pf1N1B4_2313 at 2634.111 kb on + strand, within Pf1N1B4_2313 at 2634.112 kb on - strand at 2634.112 kb on - strand at 2634.219 kb on + strand at 2634.220 kb on - strand at 2634.220 kb on - strand at 2634.220 kb on - strand at 2634.285 kb on + strand at 2634.285 kb on + strand at 2634.285 kb on + strand at 2634.288 kb on + strand at 2634.288 kb on + strand at 2634.288 kb on + strand at 2634.288 kb on + strand at 2634.289 kb on - strand at 2634.482 kb on - strand, within Pf1N1B4_2314 at 2634.845 kb on + strand, within Pf1N1B4_2314 at 2634.845 kb on + strand, within Pf1N1B4_2314 at 2634.846 kb on - strand, within Pf1N1B4_2314 at 2634.876 kb on + strand, within Pf1N1B4_2314 at 2634.920 kb on + strand at 2634.920 kb on + strand at 2634.921 kb on - strand at 2634.921 kb on - strand at 2634.921 kb on - strand at 2634.921 kb on - strand at 2634.972 kb on + strand at 2634.972 kb on + strand at 2634.973 kb on - strand at 2634.978 kb on + strand at 2634.979 kb on - strand at 2634.979 kb on - strand at 2635.042 kb on - strand at 2635.042 kb on - strand at 2635.055 kb on + strand at 2635.055 kb on + strand at 2635.055 kb on + strand at 2635.056 kb on - strand at 2635.139 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 300 mM remove 2,631,689 + +0.3 2,631,707 - Pf1N1B4_2311 0.10 -0.3 2,631,707 - Pf1N1B4_2311 0.10 +1.7 2,631,936 - -0.2 2,631,969 - +0.3 2,632,056 - +0.7 2,632,082 + +0.2 2,632,082 + +1.3 2,632,089 + -0.7 2,632,649 + -0.7 2,632,649 + +0.3 2,632,650 - -1.5 2,632,651 + +0.4 2,632,651 + -0.3 2,632,651 + -1.5 2,632,651 + -2.2 2,632,652 - +0.3 2,632,652 - -0.8 2,632,664 + -2.2 2,632,665 - +0.1 2,632,665 - -1.0 2,632,902 + Pf1N1B4_2313 0.14 +1.7 2,633,058 + Pf1N1B4_2313 0.24 +1.9 2,633,073 + Pf1N1B4_2313 0.25 -0.7 2,633,074 - Pf1N1B4_2313 0.25 -0.8 2,633,269 - Pf1N1B4_2313 0.37 +0.7 2,633,269 - Pf1N1B4_2313 0.37 +0.7 2,633,316 + Pf1N1B4_2313 0.40 +0.3 2,633,316 + Pf1N1B4_2313 0.40 -2.0 2,633,316 + Pf1N1B4_2313 0.40 -0.5 2,633,317 - Pf1N1B4_2313 0.40 -1.0 2,633,317 - +2.3 2,633,317 - Pf1N1B4_2313 0.40 -0.6 2,633,317 - Pf1N1B4_2313 0.40 -0.1 2,633,317 - Pf1N1B4_2313 0.40 -1.7 2,633,317 - Pf1N1B4_2313 0.40 +1.3 2,633,338 - Pf1N1B4_2313 0.41 +1.4 2,633,381 + Pf1N1B4_2313 0.44 +2.0 2,633,480 + Pf1N1B4_2313 0.50 +0.9 2,633,480 + Pf1N1B4_2313 0.50 -1.4 2,633,481 - Pf1N1B4_2313 0.50 +0.1 2,633,481 - Pf1N1B4_2313 0.50 +1.9 2,633,481 - Pf1N1B4_2313 0.50 -0.2 2,633,484 + Pf1N1B4_2313 0.51 -1.0 2,633,484 + Pf1N1B4_2313 0.51 +0.7 2,633,484 + Pf1N1B4_2313 0.51 -0.9 2,633,485 - Pf1N1B4_2313 0.51 +0.1 2,633,503 + Pf1N1B4_2313 0.52 -2.0 2,633,504 - Pf1N1B4_2313 0.52 +1.9 2,633,607 + Pf1N1B4_2313 0.58 -1.2 2,633,607 + Pf1N1B4_2313 0.58 -0.3 2,633,607 + Pf1N1B4_2313 0.58 -0.2 2,633,607 + Pf1N1B4_2313 0.58 -1.0 2,633,986 + Pf1N1B4_2313 0.82 +0.3 2,633,986 + Pf1N1B4_2313 0.82 +0.2 2,633,986 + Pf1N1B4_2313 0.82 +0.6 2,633,986 + Pf1N1B4_2313 0.82 -2.2 2,633,986 + Pf1N1B4_2313 0.82 -1.5 2,633,986 + Pf1N1B4_2313 0.82 +0.6 2,633,987 - Pf1N1B4_2313 0.82 +1.9 2,633,987 - Pf1N1B4_2313 0.82 -0.2 2,633,987 - Pf1N1B4_2313 0.82 -1.5 2,633,987 - Pf1N1B4_2313 0.82 -1.5 2,633,987 - Pf1N1B4_2313 0.82 -0.3 2,634,027 + Pf1N1B4_2313 0.85 +1.2 2,634,027 + Pf1N1B4_2313 0.85 +0.3 2,634,028 - Pf1N1B4_2313 0.85 -0.4 2,634,039 + Pf1N1B4_2313 0.85 -0.7 2,634,039 + Pf1N1B4_2313 0.85 +0.9 2,634,040 - Pf1N1B4_2313 0.85 -1.0 2,634,040 - Pf1N1B4_2313 0.85 -2.0 2,634,040 - Pf1N1B4_2313 0.85 -1.0 2,634,104 + Pf1N1B4_2313 0.89 +0.2 2,634,104 + Pf1N1B4_2313 0.89 +0.1 2,634,104 + Pf1N1B4_2313 0.89 -0.0 2,634,105 - Pf1N1B4_2313 0.90 -0.7 2,634,105 - Pf1N1B4_2313 0.90 +1.3 2,634,105 - Pf1N1B4_2313 0.90 -0.6 2,634,105 - Pf1N1B4_2313 0.90 -0.8 2,634,105 - Pf1N1B4_2313 0.90 -1.5 2,634,105 - Pf1N1B4_2313 0.90 +0.8 2,634,105 - Pf1N1B4_2313 0.90 +0.7 2,634,105 - Pf1N1B4_2313 0.90 +0.4 2,634,111 + Pf1N1B4_2313 0.90 -0.5 2,634,112 - +1.3 2,634,112 - -0.0 2,634,219 + +0.6 2,634,220 - +1.3 2,634,220 - -1.3 2,634,220 - +0.1 2,634,285 + +1.3 2,634,285 + -2.7 2,634,285 + -0.5 2,634,288 + -0.1 2,634,288 + -2.9 2,634,288 + +1.7 2,634,288 + +0.4 2,634,289 - -0.4 2,634,482 - Pf1N1B4_2314 0.10 +1.3 2,634,845 + Pf1N1B4_2314 0.77 -0.5 2,634,845 + Pf1N1B4_2314 0.77 +0.2 2,634,846 - Pf1N1B4_2314 0.77 -1.3 2,634,876 + Pf1N1B4_2314 0.82 -0.2 2,634,920 + -0.3 2,634,920 + -0.7 2,634,921 - -0.0 2,634,921 - -0.2 2,634,921 - +0.8 2,634,921 - -0.0 2,634,972 + +0.3 2,634,972 + +0.9 2,634,973 - +0.3 2,634,978 + -0.3 2,634,979 - +0.1 2,634,979 - +0.4 2,635,042 - -1.4 2,635,042 - +0.0 2,635,055 + +1.3 2,635,055 + +0.3 2,635,055 + +1.3 2,635,056 - -0.1 2,635,139 + +0.3
Or see this region's nucleotide sequence