Experiment: Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1687 and Pf1N1B4_1688 are separated by 170 nucleotides Pf1N1B4_1688 and Pf1N1B4_1689 are separated by 26 nucleotides Pf1N1B4_1689 and Pf1N1B4_1690 are separated by 424 nucleotides
Pf1N1B4_1687 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 1,933,956 to 1,934,696
_1687
Pf1N1B4_1688 - hypothetical protein, at 1,934,867 to 1,935,127
_1688
Pf1N1B4_1689 - High-affinity choline uptake protein BetT, at 1,935,154 to 1,937,109
_1689
Pf1N1B4_1690 - Serine acetyltransferase (EC 2.3.1.30), at 1,937,534 to 1,938,460
_1690
Position (kb)
1935
1936
1937
1938 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1934.167 kb on + strand, within Pf1N1B4_1687 at 1934.167 kb on + strand, within Pf1N1B4_1687 at 1934.167 kb on + strand, within Pf1N1B4_1687 at 1934.167 kb on + strand, within Pf1N1B4_1687 at 1934.167 kb on + strand, within Pf1N1B4_1687 at 1934.167 kb on + strand, within Pf1N1B4_1687 at 1934.167 kb on + strand, within Pf1N1B4_1687 at 1934.168 kb on - strand, within Pf1N1B4_1687 at 1934.168 kb on - strand, within Pf1N1B4_1687 at 1934.168 kb on - strand, within Pf1N1B4_1687 at 1934.168 kb on - strand, within Pf1N1B4_1687 at 1934.168 kb on - strand, within Pf1N1B4_1687 at 1934.168 kb on - strand, within Pf1N1B4_1687 at 1934.168 kb on - strand, within Pf1N1B4_1687 at 1934.168 kb on - strand, within Pf1N1B4_1687 at 1934.168 kb on - strand, within Pf1N1B4_1687 at 1934.168 kb on - strand, within Pf1N1B4_1687 at 1934.168 kb on - strand, within Pf1N1B4_1687 at 1934.415 kb on + strand, within Pf1N1B4_1687 at 1934.415 kb on + strand, within Pf1N1B4_1687 at 1934.415 kb on + strand, within Pf1N1B4_1687 at 1934.415 kb on + strand, within Pf1N1B4_1687 at 1934.416 kb on - strand, within Pf1N1B4_1687 at 1934.416 kb on - strand, within Pf1N1B4_1687 at 1934.416 kb on - strand, within Pf1N1B4_1687 at 1934.416 kb on - strand at 1934.694 kb on + strand at 1934.694 kb on + strand at 1934.695 kb on - strand at 1934.695 kb on - strand at 1934.698 kb on + strand at 1934.698 kb on + strand at 1934.698 kb on + strand at 1934.699 kb on - strand at 1934.699 kb on - strand at 1934.699 kb on - strand at 1934.699 kb on - strand at 1934.699 kb on - strand at 1934.699 kb on - strand at 1934.709 kb on + strand at 1934.709 kb on + strand at 1934.709 kb on + strand at 1934.709 kb on + strand at 1934.710 kb on - strand at 1934.710 kb on - strand at 1934.710 kb on - strand at 1934.963 kb on + strand at 1934.963 kb on + strand, within Pf1N1B4_1688 at 1934.963 kb on + strand, within Pf1N1B4_1688 at 1934.964 kb on - strand, within Pf1N1B4_1688 at 1935.246 kb on - strand at 1935.368 kb on + strand, within Pf1N1B4_1689 at 1935.368 kb on + strand, within Pf1N1B4_1689 at 1935.369 kb on - strand, within Pf1N1B4_1689 at 1935.369 kb on - strand, within Pf1N1B4_1689 at 1935.369 kb on - strand, within Pf1N1B4_1689 at 1935.369 kb on - strand, within Pf1N1B4_1689 at 1935.641 kb on + strand, within Pf1N1B4_1689 at 1935.954 kb on - strand, within Pf1N1B4_1689 at 1935.954 kb on - strand, within Pf1N1B4_1689 at 1935.987 kb on + strand, within Pf1N1B4_1689 at 1935.987 kb on + strand, within Pf1N1B4_1689 at 1935.988 kb on - strand, within Pf1N1B4_1689 at 1935.988 kb on - strand, within Pf1N1B4_1689 at 1936.178 kb on + strand, within Pf1N1B4_1689 at 1936.178 kb on + strand, within Pf1N1B4_1689 at 1936.179 kb on - strand, within Pf1N1B4_1689 at 1936.179 kb on - strand, within Pf1N1B4_1689 at 1936.220 kb on + strand, within Pf1N1B4_1689 at 1936.221 kb on - strand, within Pf1N1B4_1689 at 1936.227 kb on - strand, within Pf1N1B4_1689 at 1936.479 kb on - strand, within Pf1N1B4_1689 at 1936.479 kb on - strand, within Pf1N1B4_1689 at 1936.641 kb on + strand, within Pf1N1B4_1689 at 1936.641 kb on + strand, within Pf1N1B4_1689 at 1936.642 kb on - strand, within Pf1N1B4_1689 at 1936.642 kb on - strand, within Pf1N1B4_1689 at 1936.658 kb on + strand, within Pf1N1B4_1689 at 1936.659 kb on - strand, within Pf1N1B4_1689 at 1936.869 kb on - strand, within Pf1N1B4_1689 at 1936.869 kb on - strand, within Pf1N1B4_1689 at 1936.952 kb on + strand at 1936.952 kb on + strand at 1936.952 kb on + strand at 1936.953 kb on - strand at 1936.953 kb on - strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.973 kb on + strand at 1936.974 kb on - strand at 1936.974 kb on - strand at 1936.974 kb on - strand at 1936.974 kb on - strand at 1936.974 kb on - strand at 1936.974 kb on - strand at 1936.974 kb on - strand at 1936.976 kb on + strand at 1936.977 kb on - strand at 1936.977 kb on - strand at 1937.090 kb on + strand at 1937.090 kb on + strand at 1937.244 kb on - strand at 1937.265 kb on + strand at 1937.291 kb on - strand at 1937.309 kb on + strand at 1937.323 kb on + strand at 1937.323 kb on + strand at 1937.376 kb on + strand at 1937.380 kb on + strand at 1937.381 kb on - strand at 1937.381 kb on - strand at 1937.381 kb on - strand at 1937.458 kb on + strand at 1937.459 kb on - strand at 1937.459 kb on - strand at 1937.459 kb on - strand at 1937.535 kb on + strand at 1937.535 kb on + strand at 1937.536 kb on - strand at 1937.536 kb on - strand at 1937.536 kb on - strand at 1937.676 kb on + strand, within Pf1N1B4_1690 at 1937.676 kb on + strand, within Pf1N1B4_1690 at 1937.677 kb on - strand, within Pf1N1B4_1690 at 1937.677 kb on - strand, within Pf1N1B4_1690 at 1937.782 kb on - strand, within Pf1N1B4_1690 at 1937.782 kb on - strand, within Pf1N1B4_1690 at 1937.782 kb on - strand, within Pf1N1B4_1690 at 1938.056 kb on + strand, within Pf1N1B4_1690 at 1938.057 kb on - strand, within Pf1N1B4_1690
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 300 mM remove 1,934,167 + Pf1N1B4_1687 0.28 +0.8 1,934,167 + Pf1N1B4_1687 0.28 +0.3 1,934,167 + Pf1N1B4_1687 0.28 -0.7 1,934,167 + Pf1N1B4_1687 0.28 +0.1 1,934,167 + Pf1N1B4_1687 0.28 +0.0 1,934,167 + Pf1N1B4_1687 0.28 +0.1 1,934,167 + Pf1N1B4_1687 0.28 -0.5 1,934,168 - Pf1N1B4_1687 0.29 -1.0 1,934,168 - Pf1N1B4_1687 0.29 +0.0 1,934,168 - Pf1N1B4_1687 0.29 +0.2 1,934,168 - Pf1N1B4_1687 0.29 +0.9 1,934,168 - Pf1N1B4_1687 0.29 +1.6 1,934,168 - Pf1N1B4_1687 0.29 -0.4 1,934,168 - Pf1N1B4_1687 0.29 +0.0 1,934,168 - Pf1N1B4_1687 0.29 -0.2 1,934,168 - Pf1N1B4_1687 0.29 -2.5 1,934,168 - Pf1N1B4_1687 0.29 +1.9 1,934,168 - Pf1N1B4_1687 0.29 -1.2 1,934,415 + Pf1N1B4_1687 0.62 +1.3 1,934,415 + Pf1N1B4_1687 0.62 -1.0 1,934,415 + Pf1N1B4_1687 0.62 +1.1 1,934,415 + Pf1N1B4_1687 0.62 +0.3 1,934,416 - Pf1N1B4_1687 0.62 +0.2 1,934,416 - Pf1N1B4_1687 0.62 +0.9 1,934,416 - Pf1N1B4_1687 0.62 -1.1 1,934,416 - -0.2 1,934,694 + -0.2 1,934,694 + -1.7 1,934,695 - +0.3 1,934,695 - +1.3 1,934,698 + +0.3 1,934,698 + +1.3 1,934,698 + -0.5 1,934,699 - +2.3 1,934,699 - +0.3 1,934,699 - +1.3 1,934,699 - -1.7 1,934,699 - -1.7 1,934,699 - +2.3 1,934,709 + -1.0 1,934,709 + -1.4 1,934,709 + -1.0 1,934,709 + -1.0 1,934,710 - -0.2 1,934,710 - -1.0 1,934,710 - -0.7 1,934,963 + +0.3 1,934,963 + Pf1N1B4_1688 0.37 -2.2 1,934,963 + Pf1N1B4_1688 0.37 +0.9 1,934,964 - Pf1N1B4_1688 0.37 -0.7 1,935,246 - +0.3 1,935,368 + Pf1N1B4_1689 0.11 -0.8 1,935,368 + Pf1N1B4_1689 0.11 -0.2 1,935,369 - Pf1N1B4_1689 0.11 -1.0 1,935,369 - Pf1N1B4_1689 0.11 +1.3 1,935,369 - Pf1N1B4_1689 0.11 -1.1 1,935,369 - Pf1N1B4_1689 0.11 +0.3 1,935,641 + Pf1N1B4_1689 0.25 -1.8 1,935,954 - Pf1N1B4_1689 0.41 -0.9 1,935,954 - Pf1N1B4_1689 0.41 +0.3 1,935,987 + Pf1N1B4_1689 0.43 +0.0 1,935,987 + Pf1N1B4_1689 0.43 -1.2 1,935,988 - Pf1N1B4_1689 0.43 -1.8 1,935,988 - Pf1N1B4_1689 0.43 -1.4 1,936,178 + Pf1N1B4_1689 0.52 -1.7 1,936,178 + Pf1N1B4_1689 0.52 +0.1 1,936,179 - Pf1N1B4_1689 0.52 -2.0 1,936,179 - Pf1N1B4_1689 0.52 -1.7 1,936,220 + Pf1N1B4_1689 0.54 -1.5 1,936,221 - Pf1N1B4_1689 0.55 -0.8 1,936,227 - Pf1N1B4_1689 0.55 +0.0 1,936,479 - Pf1N1B4_1689 0.68 -0.7 1,936,479 - Pf1N1B4_1689 0.68 +0.3 1,936,641 + Pf1N1B4_1689 0.76 -0.8 1,936,641 + Pf1N1B4_1689 0.76 +0.1 1,936,642 - Pf1N1B4_1689 0.76 -1.3 1,936,642 - Pf1N1B4_1689 0.76 +1.9 1,936,658 + Pf1N1B4_1689 0.77 +0.1 1,936,659 - Pf1N1B4_1689 0.77 -0.9 1,936,869 - Pf1N1B4_1689 0.88 -0.7 1,936,869 - Pf1N1B4_1689 0.88 +0.8 1,936,952 + -0.7 1,936,952 + -0.9 1,936,952 + -1.5 1,936,953 - +0.3 1,936,953 - +1.0 1,936,973 + -1.8 1,936,973 + -1.0 1,936,973 + -1.8 1,936,973 + -2.0 1,936,973 + -1.5 1,936,973 + -1.3 1,936,973 + -1.8 1,936,973 + -0.2 1,936,974 - -1.1 1,936,974 - -1.7 1,936,974 - +0.7 1,936,974 - -1.0 1,936,974 - -0.7 1,936,974 - +0.3 1,936,974 - -2.0 1,936,976 + -0.2 1,936,977 - -1.0 1,936,977 - -0.7 1,937,090 + -0.8 1,937,090 + +0.5 1,937,244 - +0.1 1,937,265 + +0.5 1,937,291 - -0.8 1,937,309 + +1.3 1,937,323 + +1.3 1,937,323 + +1.3 1,937,376 + -0.5 1,937,380 + -0.6 1,937,381 - +1.2 1,937,381 - -0.8 1,937,381 - -0.4 1,937,458 + +1.8 1,937,459 - +2.6 1,937,459 - -0.5 1,937,459 - -0.2 1,937,535 + -0.0 1,937,535 + -0.2 1,937,536 - -1.4 1,937,536 - +0.3 1,937,536 - -2.3 1,937,676 + Pf1N1B4_1690 0.15 +0.0 1,937,676 + Pf1N1B4_1690 0.15 -0.8 1,937,677 - Pf1N1B4_1690 0.15 -0.5 1,937,677 - Pf1N1B4_1690 0.15 -0.1 1,937,782 - Pf1N1B4_1690 0.27 -0.8 1,937,782 - Pf1N1B4_1690 0.27 -1.8 1,937,782 - Pf1N1B4_1690 0.27 +0.9 1,938,056 + Pf1N1B4_1690 0.56 -0.2 1,938,057 - Pf1N1B4_1690 0.56 -0.5
Or see this region's nucleotide sequence