Experiment: Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1127 and Pf1N1B4_1128 are separated by 169 nucleotides Pf1N1B4_1128 and Pf1N1B4_1129 are separated by 351 nucleotides Pf1N1B4_1129 and Pf1N1B4_1130 overlap by 19 nucleotides Pf1N1B4_1130 and Pf1N1B4_1131 are separated by 502 nucleotides
Pf1N1B4_1127 - Dipeptide transport ATP-binding protein DppF (TC 3.A.1.5.2), at 1,265,723 to 1,266,703
_1127
Pf1N1B4_1128 - hypothetical protein, at 1,266,873 to 1,267,088
_1128
Pf1N1B4_1129 - Peptide chain release factor 3, at 1,267,440 to 1,267,691
_1129
Pf1N1B4_1130 - Peptide chain release factor 3, at 1,267,673 to 1,269,022
_1130
Pf1N1B4_1131 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,269,525 to 1,270,241
_1131
Position (kb)
1267
1268
1269
1270 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1266.701 kb on + strand at 1266.874 kb on + strand at 1266.874 kb on + strand at 1266.875 kb on - strand at 1266.875 kb on - strand at 1266.875 kb on - strand at 1267.055 kb on + strand, within Pf1N1B4_1128 at 1267.055 kb on + strand, within Pf1N1B4_1128 at 1267.055 kb on + strand, within Pf1N1B4_1128 at 1267.056 kb on - strand, within Pf1N1B4_1128 at 1267.056 kb on - strand, within Pf1N1B4_1128 at 1267.078 kb on + strand at 1267.078 kb on + strand at 1267.078 kb on + strand at 1267.078 kb on + strand at 1267.079 kb on - strand at 1267.079 kb on - strand at 1267.079 kb on - strand at 1267.082 kb on + strand at 1267.082 kb on + strand at 1267.082 kb on + strand at 1267.082 kb on + strand at 1267.082 kb on + strand at 1267.082 kb on + strand at 1267.082 kb on + strand at 1267.083 kb on - strand at 1267.083 kb on - strand at 1267.083 kb on - strand at 1267.083 kb on - strand at 1267.088 kb on + strand at 1267.088 kb on + strand at 1267.088 kb on + strand at 1267.088 kb on + strand at 1267.088 kb on + strand at 1267.088 kb on + strand at 1267.089 kb on - strand at 1267.134 kb on + strand at 1267.148 kb on + strand at 1267.148 kb on + strand at 1267.149 kb on - strand at 1267.235 kb on + strand at 1267.235 kb on + strand at 1267.236 kb on - strand at 1267.237 kb on + strand at 1267.407 kb on - strand at 1267.407 kb on - strand at 1267.407 kb on - strand at 1267.428 kb on - strand at 1269.150 kb on + strand at 1269.150 kb on + strand at 1269.170 kb on + strand at 1269.170 kb on + strand at 1269.170 kb on + strand at 1269.170 kb on + strand at 1269.197 kb on + strand at 1269.258 kb on + strand at 1269.258 kb on + strand at 1269.258 kb on + strand at 1269.259 kb on - strand at 1269.284 kb on - strand at 1269.284 kb on - strand at 1269.287 kb on + strand at 1269.287 kb on + strand at 1269.287 kb on + strand at 1269.288 kb on - strand at 1269.288 kb on - strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.301 kb on + strand at 1269.301 kb on + strand at 1269.301 kb on + strand at 1269.301 kb on + strand at 1269.302 kb on - strand at 1269.313 kb on + strand at 1269.313 kb on + strand at 1269.313 kb on + strand at 1269.313 kb on + strand at 1269.314 kb on - strand at 1269.314 kb on - strand at 1269.352 kb on + strand at 1269.352 kb on + strand at 1269.353 kb on - strand at 1269.399 kb on - strand at 1269.422 kb on - strand at 1269.537 kb on + strand at 1269.553 kb on + strand at 1269.553 kb on + strand at 1269.554 kb on - strand at 1269.645 kb on + strand, within Pf1N1B4_1131 at 1269.697 kb on + strand, within Pf1N1B4_1131 at 1269.697 kb on + strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.806 kb on - strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.901 kb on - strand, within Pf1N1B4_1131 at 1269.901 kb on - strand, within Pf1N1B4_1131 at 1269.977 kb on - strand, within Pf1N1B4_1131
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 300 mM remove 1,266,701 + -1.1 1,266,874 + +0.0 1,266,874 + -0.4 1,266,875 - +0.2 1,266,875 - +0.6 1,266,875 - -1.1 1,267,055 + Pf1N1B4_1128 0.84 +0.3 1,267,055 + Pf1N1B4_1128 0.84 +0.3 1,267,055 + Pf1N1B4_1128 0.84 +0.8 1,267,056 - Pf1N1B4_1128 0.85 -1.3 1,267,056 - Pf1N1B4_1128 0.85 -1.2 1,267,078 + +0.8 1,267,078 + -0.6 1,267,078 + +0.6 1,267,078 + -1.0 1,267,079 - -0.4 1,267,079 - -0.4 1,267,079 - +0.8 1,267,082 + +1.0 1,267,082 + +1.4 1,267,082 + +0.8 1,267,082 + +0.2 1,267,082 + -2.7 1,267,082 + +0.6 1,267,082 + +1.2 1,267,083 - -0.6 1,267,083 - +0.4 1,267,083 - -0.4 1,267,083 - -1.4 1,267,088 + +0.8 1,267,088 + -1.3 1,267,088 + -0.1 1,267,088 + +1.2 1,267,088 + +0.0 1,267,088 + -0.3 1,267,089 - -0.2 1,267,134 + +0.6 1,267,148 + +0.4 1,267,148 + +0.9 1,267,149 - +0.6 1,267,235 + +0.6 1,267,235 + -1.4 1,267,236 - -1.1 1,267,237 + -0.6 1,267,407 - +1.6 1,267,407 - -1.6 1,267,407 - -1.1 1,267,428 - -1.1 1,269,150 + +0.2 1,269,150 + +0.0 1,269,170 + -0.4 1,269,170 + -0.0 1,269,170 + +0.9 1,269,170 + +1.6 1,269,197 + +0.6 1,269,258 + +2.8 1,269,258 + +1.2 1,269,258 + -0.2 1,269,259 - +0.2 1,269,284 - -1.1 1,269,284 - +0.2 1,269,287 + +0.0 1,269,287 + -0.2 1,269,287 + +0.2 1,269,288 - +0.6 1,269,288 - -1.4 1,269,297 + +0.4 1,269,297 + +0.8 1,269,297 + +1.2 1,269,297 + +0.9 1,269,297 + +0.6 1,269,297 + +0.3 1,269,301 + -0.1 1,269,301 + +1.1 1,269,301 + +0.1 1,269,301 + +0.5 1,269,302 - +0.2 1,269,313 + +1.2 1,269,313 + +1.1 1,269,313 + +1.7 1,269,313 + +2.2 1,269,314 - -1.6 1,269,314 - +1.2 1,269,352 + -0.4 1,269,352 + -0.1 1,269,353 - -1.4 1,269,399 - -0.1 1,269,422 - -2.0 1,269,537 + +1.9 1,269,553 + -0.4 1,269,553 + -2.6 1,269,554 - -1.3 1,269,645 + Pf1N1B4_1131 0.17 -0.0 1,269,697 + Pf1N1B4_1131 0.24 -0.1 1,269,697 + Pf1N1B4_1131 0.24 -1.8 1,269,698 - Pf1N1B4_1131 0.24 +2.5 1,269,698 - Pf1N1B4_1131 0.24 -1.2 1,269,698 - Pf1N1B4_1131 0.24 +0.2 1,269,698 - Pf1N1B4_1131 0.24 +0.2 1,269,698 - Pf1N1B4_1131 0.24 -2.0 1,269,806 - Pf1N1B4_1131 0.39 -1.6 1,269,900 + Pf1N1B4_1131 0.52 -2.1 1,269,900 + Pf1N1B4_1131 0.52 -0.3 1,269,900 + Pf1N1B4_1131 0.52 -0.8 1,269,900 + Pf1N1B4_1131 0.52 -2.3 1,269,900 + Pf1N1B4_1131 0.52 +0.0 1,269,901 - Pf1N1B4_1131 0.52 -1.1 1,269,901 - Pf1N1B4_1131 0.52 -1.5 1,269,977 - Pf1N1B4_1131 0.63 -1.3
Or see this region's nucleotide sequence