Experiment: PYE with Benzalkonium Chloride 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02223 and CCNA_02224 overlap by 4 nucleotides CCNA_02224 and CCNA_02225 overlap by 4 nucleotides CCNA_02225 and CCNA_02226 are separated by 43 nucleotides CCNA_02226 and CCNA_02227 are separated by 31 nucleotides
CCNA_02223: CCNA_02223 - beta-lactamase, type II, at 2,371,873 to 2,372,742
_02223
CCNA_02224: CCNA_02224 - methylenetetrahydrofolate reductase, at 2,372,739 to 2,373,683
_02224
CCNA_02225: CCNA_02225 - ArsR family/SAM-dependent transcriptional regulator, at 2,373,680 to 2,374,657
_02225
CCNA_02226: CCNA_02226 - glyoxalase family protein, at 2,374,701 to 2,375,078
_02226
CCNA_02227: CCNA_02227 - LysR-family transcriptional regulator, at 2,375,110 to 2,375,982
_02227
Position (kb)
2373
2374
2375 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2372.690 kb on + strand at 2372.735 kb on - strand at 2372.750 kb on + strand at 2372.750 kb on + strand at 2372.771 kb on - strand at 2372.780 kb on + strand at 2372.781 kb on + strand at 2372.786 kb on + strand at 2372.794 kb on - strand at 2372.820 kb on + strand at 2372.827 kb on - strand at 2372.833 kb on + strand at 2372.853 kb on - strand, within CCNA_02224 at 2372.991 kb on + strand, within CCNA_02224 at 2373.024 kb on - strand, within CCNA_02224 at 2373.097 kb on + strand, within CCNA_02224 at 2373.196 kb on + strand, within CCNA_02224 at 2373.199 kb on - strand, within CCNA_02224 at 2373.199 kb on - strand, within CCNA_02224 at 2373.202 kb on + strand, within CCNA_02224 at 2373.202 kb on + strand, within CCNA_02224 at 2373.210 kb on - strand, within CCNA_02224 at 2373.214 kb on + strand, within CCNA_02224 at 2373.224 kb on + strand, within CCNA_02224 at 2373.241 kb on + strand, within CCNA_02224 at 2373.241 kb on + strand, within CCNA_02224 at 2373.249 kb on - strand, within CCNA_02224 at 2373.249 kb on - strand, within CCNA_02224 at 2373.254 kb on - strand, within CCNA_02224 at 2373.272 kb on - strand, within CCNA_02224 at 2373.273 kb on - strand, within CCNA_02224 at 2373.284 kb on + strand, within CCNA_02224 at 2373.414 kb on + strand, within CCNA_02224 at 2373.414 kb on + strand, within CCNA_02224 at 2373.422 kb on - strand, within CCNA_02224 at 2373.422 kb on - strand, within CCNA_02224 at 2373.439 kb on - strand, within CCNA_02224 at 2373.450 kb on - strand, within CCNA_02224 at 2373.500 kb on + strand, within CCNA_02224 at 2373.500 kb on + strand, within CCNA_02224 at 2373.508 kb on - strand, within CCNA_02224 at 2373.508 kb on - strand, within CCNA_02224 at 2373.508 kb on - strand, within CCNA_02224 at 2373.508 kb on - strand, within CCNA_02224 at 2373.510 kb on - strand, within CCNA_02224 at 2373.539 kb on + strand, within CCNA_02224 at 2373.543 kb on + strand, within CCNA_02224 at 2373.575 kb on - strand, within CCNA_02224 at 2373.583 kb on + strand, within CCNA_02224 at 2373.583 kb on + strand, within CCNA_02224 at 2373.583 kb on + strand, within CCNA_02224 at 2373.591 kb on - strand at 2373.667 kb on - strand at 2373.671 kb on + strand at 2373.692 kb on + strand at 2373.694 kb on + strand at 2373.700 kb on - strand at 2373.700 kb on - strand at 2373.734 kb on - strand at 2373.734 kb on - strand at 2373.787 kb on + strand, within CCNA_02225 at 2373.818 kb on + strand, within CCNA_02225 at 2373.856 kb on - strand, within CCNA_02225 at 2373.944 kb on - strand, within CCNA_02225 at 2373.971 kb on + strand, within CCNA_02225 at 2373.995 kb on - strand, within CCNA_02225 at 2374.100 kb on - strand, within CCNA_02225 at 2374.122 kb on - strand, within CCNA_02225 at 2374.249 kb on + strand, within CCNA_02225 at 2374.257 kb on - strand, within CCNA_02225 at 2374.299 kb on + strand, within CCNA_02225 at 2374.307 kb on - strand, within CCNA_02225 at 2374.317 kb on + strand, within CCNA_02225 at 2374.324 kb on - strand, within CCNA_02225 at 2374.353 kb on + strand, within CCNA_02225 at 2374.458 kb on - strand, within CCNA_02225 at 2374.458 kb on - strand, within CCNA_02225 at 2374.458 kb on - strand, within CCNA_02225 at 2374.458 kb on - strand, within CCNA_02225 at 2374.499 kb on - strand, within CCNA_02225 at 2374.539 kb on - strand, within CCNA_02225 at 2374.558 kb on - strand, within CCNA_02225 at 2374.738 kb on - strand at 2374.741 kb on + strand, within CCNA_02226 at 2374.768 kb on - strand, within CCNA_02226 at 2374.768 kb on - strand, within CCNA_02226 at 2374.818 kb on - strand, within CCNA_02226 at 2374.831 kb on + strand, within CCNA_02226 at 2374.831 kb on + strand, within CCNA_02226 at 2374.839 kb on - strand, within CCNA_02226 at 2374.839 kb on - strand, within CCNA_02226 at 2374.844 kb on - strand, within CCNA_02226 at 2374.862 kb on - strand, within CCNA_02226 at 2374.902 kb on + strand, within CCNA_02226 at 2374.902 kb on + strand, within CCNA_02226 at 2374.910 kb on - strand, within CCNA_02226 at 2374.942 kb on - strand, within CCNA_02226 at 2374.945 kb on + strand, within CCNA_02226 at 2374.948 kb on - strand, within CCNA_02226 at 2374.977 kb on + strand, within CCNA_02226 at 2375.000 kb on - strand, within CCNA_02226 at 2375.003 kb on + strand, within CCNA_02226 at 2375.013 kb on + strand, within CCNA_02226 at 2375.021 kb on - strand, within CCNA_02226 at 2375.023 kb on + strand, within CCNA_02226 at 2375.023 kb on + strand, within CCNA_02226 at 2375.031 kb on - strand, within CCNA_02226 at 2375.031 kb on - strand, within CCNA_02226 at 2375.031 kb on - strand, within CCNA_02226 at 2375.031 kb on - strand, within CCNA_02226 at 2375.043 kb on + strand at 2375.043 kb on + strand at 2375.051 kb on - strand at 2375.061 kb on + strand at 2375.068 kb on + strand at 2375.069 kb on - strand at 2375.076 kb on - strand at 2375.076 kb on - strand at 2375.095 kb on - strand at 2375.095 kb on - strand at 2375.095 kb on - strand at 2375.101 kb on + strand at 2375.149 kb on - strand at 2375.194 kb on - strand at 2375.225 kb on + strand, within CCNA_02227 at 2375.256 kb on - strand, within CCNA_02227 at 2375.307 kb on + strand, within CCNA_02227 at 2375.315 kb on - strand, within CCNA_02227 at 2375.329 kb on - strand, within CCNA_02227 at 2375.380 kb on + strand, within CCNA_02227 at 2375.407 kb on - strand, within CCNA_02227 at 2375.430 kb on + strand, within CCNA_02227 at 2375.438 kb on - strand, within CCNA_02227 at 2375.444 kb on + strand, within CCNA_02227 at 2375.444 kb on + strand, within CCNA_02227 at 2375.452 kb on - strand, within CCNA_02227 at 2375.468 kb on + strand, within CCNA_02227 at 2375.475 kb on - strand, within CCNA_02227 at 2375.496 kb on - strand, within CCNA_02227 at 2375.497 kb on - strand, within CCNA_02227 at 2375.518 kb on + strand, within CCNA_02227 at 2375.526 kb on - strand, within CCNA_02227 at 2375.527 kb on - strand, within CCNA_02227 at 2375.537 kb on - strand, within CCNA_02227 at 2375.537 kb on - strand, within CCNA_02227 at 2375.612 kb on + strand, within CCNA_02227 at 2375.612 kb on + strand, within CCNA_02227
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Benzalkonium Chloride 0.0001 mg/ml remove 2,372,690 + -0.8 2,372,735 - +0.3 2,372,750 + -1.1 2,372,750 + +1.1 2,372,771 - -0.1 2,372,780 + -0.5 2,372,781 + -0.3 2,372,786 + -0.9 2,372,794 - +3.6 2,372,820 + -1.0 2,372,827 - +0.3 2,372,833 + -1.9 2,372,853 - CCNA_02224 0.12 +0.9 2,372,991 + CCNA_02224 0.27 +0.4 2,373,024 - CCNA_02224 0.30 +1.8 2,373,097 + CCNA_02224 0.38 -1.2 2,373,196 + CCNA_02224 0.48 +1.7 2,373,199 - CCNA_02224 0.49 +0.3 2,373,199 - CCNA_02224 0.49 +0.4 2,373,202 + CCNA_02224 0.49 +0.4 2,373,202 + CCNA_02224 0.49 -0.8 2,373,210 - CCNA_02224 0.50 -0.3 2,373,214 + CCNA_02224 0.50 -1.8 2,373,224 + CCNA_02224 0.51 -0.1 2,373,241 + CCNA_02224 0.53 -3.3 2,373,241 + CCNA_02224 0.53 -1.8 2,373,249 - CCNA_02224 0.54 -0.2 2,373,249 - CCNA_02224 0.54 -0.0 2,373,254 - CCNA_02224 0.54 +1.2 2,373,272 - CCNA_02224 0.56 -0.5 2,373,273 - CCNA_02224 0.57 +1.1 2,373,284 + CCNA_02224 0.58 -1.1 2,373,414 + CCNA_02224 0.71 -0.4 2,373,414 + CCNA_02224 0.71 -1.0 2,373,422 - CCNA_02224 0.72 -0.6 2,373,422 - CCNA_02224 0.72 -1.3 2,373,439 - CCNA_02224 0.74 +0.6 2,373,450 - CCNA_02224 0.75 +0.0 2,373,500 + CCNA_02224 0.81 -0.1 2,373,500 + CCNA_02224 0.81 -0.4 2,373,508 - CCNA_02224 0.81 +0.3 2,373,508 - CCNA_02224 0.81 +0.7 2,373,508 - CCNA_02224 0.81 -0.9 2,373,508 - CCNA_02224 0.81 -0.1 2,373,510 - CCNA_02224 0.82 -0.4 2,373,539 + CCNA_02224 0.85 -2.0 2,373,543 + CCNA_02224 0.85 -0.8 2,373,575 - CCNA_02224 0.88 -1.4 2,373,583 + CCNA_02224 0.89 -0.0 2,373,583 + CCNA_02224 0.89 -0.3 2,373,583 + CCNA_02224 0.89 -0.9 2,373,591 - -1.4 2,373,667 - -0.6 2,373,671 + -1.4 2,373,692 + -1.5 2,373,694 + -1.3 2,373,700 - +0.8 2,373,700 - +0.8 2,373,734 - +2.8 2,373,734 - +2.3 2,373,787 + CCNA_02225 0.11 +0.2 2,373,818 + CCNA_02225 0.14 +0.7 2,373,856 - CCNA_02225 0.18 +2.7 2,373,944 - CCNA_02225 0.27 +2.1 2,373,971 + CCNA_02225 0.30 -0.6 2,373,995 - CCNA_02225 0.32 +2.6 2,374,100 - CCNA_02225 0.43 +1.3 2,374,122 - CCNA_02225 0.45 +3.0 2,374,249 + CCNA_02225 0.58 -0.0 2,374,257 - CCNA_02225 0.59 +2.7 2,374,299 + CCNA_02225 0.63 +1.0 2,374,307 - CCNA_02225 0.64 +2.4 2,374,317 + CCNA_02225 0.65 -0.1 2,374,324 - CCNA_02225 0.66 +3.2 2,374,353 + CCNA_02225 0.69 +1.0 2,374,458 - CCNA_02225 0.80 +3.6 2,374,458 - CCNA_02225 0.80 +3.4 2,374,458 - CCNA_02225 0.80 +3.3 2,374,458 - CCNA_02225 0.80 +3.7 2,374,499 - CCNA_02225 0.84 +3.4 2,374,539 - CCNA_02225 0.88 +3.3 2,374,558 - CCNA_02225 0.90 +3.5 2,374,738 - -0.7 2,374,741 + CCNA_02226 0.11 +0.4 2,374,768 - CCNA_02226 0.18 -2.6 2,374,768 - CCNA_02226 0.18 -0.9 2,374,818 - CCNA_02226 0.31 +1.2 2,374,831 + CCNA_02226 0.34 +2.8 2,374,831 + CCNA_02226 0.34 -0.9 2,374,839 - CCNA_02226 0.37 +1.0 2,374,839 - CCNA_02226 0.37 +1.0 2,374,844 - CCNA_02226 0.38 +0.1 2,374,862 - CCNA_02226 0.43 +1.1 2,374,902 + CCNA_02226 0.53 +0.8 2,374,902 + CCNA_02226 0.53 -0.5 2,374,910 - CCNA_02226 0.55 -2.2 2,374,942 - CCNA_02226 0.64 -0.5 2,374,945 + CCNA_02226 0.65 -2.1 2,374,948 - CCNA_02226 0.65 -1.7 2,374,977 + CCNA_02226 0.73 -0.3 2,375,000 - CCNA_02226 0.79 -1.0 2,375,003 + CCNA_02226 0.80 -0.3 2,375,013 + CCNA_02226 0.83 -0.1 2,375,021 - CCNA_02226 0.85 -0.5 2,375,023 + CCNA_02226 0.85 -1.1 2,375,023 + CCNA_02226 0.85 -2.5 2,375,031 - CCNA_02226 0.87 +0.8 2,375,031 - CCNA_02226 0.87 -0.1 2,375,031 - CCNA_02226 0.87 +0.6 2,375,031 - CCNA_02226 0.87 -1.6 2,375,043 + +0.6 2,375,043 + -1.5 2,375,051 - +0.2 2,375,061 + -0.3 2,375,068 + -0.2 2,375,069 - -0.8 2,375,076 - -0.6 2,375,076 - -1.6 2,375,095 - -0.7 2,375,095 - -0.5 2,375,095 - -1.9 2,375,101 + -0.8 2,375,149 - +0.8 2,375,194 - +0.6 2,375,225 + CCNA_02227 0.13 -2.3 2,375,256 - CCNA_02227 0.17 +0.1 2,375,307 + CCNA_02227 0.23 -0.0 2,375,315 - CCNA_02227 0.23 +0.4 2,375,329 - CCNA_02227 0.25 +0.6 2,375,380 + CCNA_02227 0.31 +2.7 2,375,407 - CCNA_02227 0.34 +0.9 2,375,430 + CCNA_02227 0.37 -2.0 2,375,438 - CCNA_02227 0.38 -0.0 2,375,444 + CCNA_02227 0.38 -0.3 2,375,444 + CCNA_02227 0.38 -0.6 2,375,452 - CCNA_02227 0.39 +0.2 2,375,468 + CCNA_02227 0.41 +0.1 2,375,475 - CCNA_02227 0.42 +0.2 2,375,496 - CCNA_02227 0.44 +0.6 2,375,497 - CCNA_02227 0.44 +0.5 2,375,518 + CCNA_02227 0.47 +1.0 2,375,526 - CCNA_02227 0.48 -0.4 2,375,527 - CCNA_02227 0.48 -1.9 2,375,537 - CCNA_02227 0.49 -1.7 2,375,537 - CCNA_02227 0.49 -0.0 2,375,612 + CCNA_02227 0.58 -2.4 2,375,612 + CCNA_02227 0.58 -1.6
Or see this region's nucleotide sequence