Experiment: PYE with Benzalkonium Chloride 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01957 and CCNA_01958 are separated by 144 nucleotides CCNA_01958 and CCNA_01959 are separated by 78 nucleotides CCNA_01959 and CCNA_01960 are separated by 60 nucleotides CCNA_01960 and CCNA_01961 are separated by 5 nucleotides
CCNA_01957: CCNA_01957 - thiazole biosynthesis protein ThiG, at 2,102,616 to 2,103,425
_01957
CCNA_01958: CCNA_01958 - ThiaminS ubiquitin-like sulfur carrier protein ThiS, at 2,103,570 to 2,103,770
_01958
CCNA_01959: CCNA_01959 - 3-dehydroquinate dehydratase, at 2,103,849 to 2,104,289
_01959
CCNA_01960: CCNA_01960 - acetyl-CoA carboxylase biotin carboxyl carrier protein subunit, at 2,104,350 to 2,104,862
_01960
CCNA_01961: CCNA_01961 - biotin carboxylase, at 2,104,868 to 2,106,214
_01961
Position (kb)
2103
2104
2105 Strain fitness (log2 ratio)
-1
0
1
2 at 2103.440 kb on - strand at 2103.496 kb on + strand at 2103.496 kb on + strand at 2103.504 kb on - strand at 2103.514 kb on + strand at 2103.537 kb on - strand at 2103.550 kb on + strand at 2103.568 kb on + strand at 2103.776 kb on - strand at 2103.793 kb on - strand at 2103.816 kb on + strand at 2103.816 kb on + strand at 2103.953 kb on + strand, within CCNA_01959 at 2104.139 kb on + strand, within CCNA_01959 at 2104.149 kb on + strand, within CCNA_01959 at 2104.154 kb on + strand, within CCNA_01959 at 2104.154 kb on + strand, within CCNA_01959 at 2104.221 kb on + strand, within CCNA_01959 at 2104.267 kb on + strand at 2104.293 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Benzalkonium Chloride 0.0001 mg/ml remove 2,103,440 - +1.2 2,103,496 + +0.4 2,103,496 + +0.2 2,103,504 - +0.6 2,103,514 + -1.2 2,103,537 - -0.8 2,103,550 + -0.7 2,103,568 + +1.5 2,103,776 - +0.4 2,103,793 - -0.4 2,103,816 + -1.2 2,103,816 + +0.6 2,103,953 + CCNA_01959 0.24 -0.1 2,104,139 + CCNA_01959 0.66 +2.5 2,104,149 + CCNA_01959 0.68 +2.6 2,104,154 + CCNA_01959 0.69 +1.5 2,104,154 + CCNA_01959 0.69 +2.3 2,104,221 + CCNA_01959 0.84 +0.5 2,104,267 + -1.0 2,104,293 + +0.2
Or see this region's nucleotide sequence