Experiment: PYE with Benzalkonium Chloride 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00323 and CCNA_00324 are separated by 203 nucleotides CCNA_00324 and CCNA_00326 are separated by 243 nucleotides CCNA_00326 and CCNA_00327 are separated by 162 nucleotides CCNA_00327 and CCNA_00328 are separated by 84 nucleotides
CCNA_00323: CCNA_00323 - low-affinity zinc transport protein, at 336,631 to 337,728
_00323
CCNA_00324: CCNA_00324 - ExbD-family protein, at 337,932 to 338,390
_00324
CCNA_00326: CCNA_00326 - vegetatible incompatibility protein HET-E-1, at 338,634 to 339,665
_00326
CCNA_00327: CCNA_00327 - FMN-binding nitroreductase family enzyme, at 339,828 to 340,490
_00327
CCNA_00328: CCNA_00328 - monofunctional biosynthetic peptidoglycan transglycosylase MtgA, at 340,575 to 341,264
_00328
Position (kb) 338
339
340 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 337.644 kb on - strand at 337.663 kb on + strand at 337.671 kb on - strand at 337.688 kb on + strand at 337.731 kb on + strand at 337.732 kb on + strand at 337.747 kb on - strand at 337.759 kb on + strand at 337.779 kb on - strand at 337.795 kb on + strand at 337.796 kb on + strand at 337.803 kb on + strand at 337.803 kb on + strand at 337.811 kb on - strand at 337.816 kb on - strand at 337.830 kb on + strand at 337.837 kb on + strand at 337.842 kb on + strand at 337.842 kb on + strand at 337.842 kb on + strand at 337.842 kb on + strand at 337.850 kb on - strand at 337.850 kb on - strand at 337.850 kb on - strand at 337.891 kb on - strand at 337.961 kb on + strand at 337.963 kb on - strand at 337.963 kb on - strand at 337.999 kb on + strand, within CCNA_00324 at 337.999 kb on + strand, within CCNA_00324 at 338.011 kb on + strand, within CCNA_00324 at 338.228 kb on + strand, within CCNA_00324 at 338.244 kb on + strand, within CCNA_00324 at 338.252 kb on - strand, within CCNA_00324 at 338.261 kb on - strand, within CCNA_00324 at 338.309 kb on + strand, within CCNA_00324 at 338.382 kb on + strand at 338.385 kb on - strand at 338.385 kb on - strand at 338.390 kb on - strand at 338.390 kb on - strand at 338.400 kb on - strand at 338.452 kb on + strand at 338.455 kb on - strand at 338.460 kb on - strand at 338.603 kb on - strand at 338.604 kb on - strand at 338.604 kb on - strand at 338.618 kb on + strand at 338.618 kb on + strand at 338.618 kb on + strand at 338.618 kb on + strand at 338.618 kb on + strand at 338.626 kb on - strand at 338.626 kb on - strand at 338.626 kb on - strand at 338.731 kb on - strand at 338.741 kb on - strand, within CCNA_00326 at 338.759 kb on - strand, within CCNA_00326 at 338.784 kb on + strand, within CCNA_00326 at 338.792 kb on - strand, within CCNA_00326 at 338.804 kb on + strand, within CCNA_00326 at 338.850 kb on + strand, within CCNA_00326 at 338.881 kb on + strand, within CCNA_00326 at 338.941 kb on + strand, within CCNA_00326 at 338.946 kb on + strand, within CCNA_00326 at 338.997 kb on + strand, within CCNA_00326 at 339.005 kb on - strand, within CCNA_00326 at 339.016 kb on + strand, within CCNA_00326 at 339.042 kb on + strand, within CCNA_00326 at 339.046 kb on - strand, within CCNA_00326 at 339.050 kb on - strand, within CCNA_00326 at 339.096 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.146 kb on - strand, within CCNA_00326 at 339.146 kb on - strand, within CCNA_00326 at 339.146 kb on - strand, within CCNA_00326 at 339.191 kb on + strand, within CCNA_00326 at 339.289 kb on + strand, within CCNA_00326 at 339.308 kb on + strand, within CCNA_00326 at 339.308 kb on + strand, within CCNA_00326 at 339.317 kb on - strand, within CCNA_00326 at 339.327 kb on + strand, within CCNA_00326 at 339.347 kb on + strand, within CCNA_00326 at 339.347 kb on + strand, within CCNA_00326 at 339.355 kb on - strand, within CCNA_00326 at 339.415 kb on + strand, within CCNA_00326 at 339.423 kb on - strand, within CCNA_00326 at 339.468 kb on + strand, within CCNA_00326 at 339.468 kb on + strand, within CCNA_00326 at 339.485 kb on + strand, within CCNA_00326 at 339.485 kb on + strand, within CCNA_00326 at 339.485 kb on + strand, within CCNA_00326 at 339.488 kb on - strand, within CCNA_00326 at 339.493 kb on - strand, within CCNA_00326 at 339.493 kb on - strand, within CCNA_00326 at 339.501 kb on - strand, within CCNA_00326 at 339.524 kb on + strand, within CCNA_00326 at 339.531 kb on + strand, within CCNA_00326 at 339.532 kb on - strand, within CCNA_00326 at 339.532 kb on - strand, within CCNA_00326 at 339.539 kb on - strand, within CCNA_00326 at 339.590 kb on - strand at 339.626 kb on - strand at 339.629 kb on - strand at 339.660 kb on + strand at 339.661 kb on - strand at 339.681 kb on + strand at 339.691 kb on + strand at 339.699 kb on - strand at 339.735 kb on + strand at 339.769 kb on + strand at 339.769 kb on + strand at 339.791 kb on + strand at 339.806 kb on + strand at 339.806 kb on + strand at 339.806 kb on + strand at 339.814 kb on - strand at 339.816 kb on + strand at 339.875 kb on + strand at 339.915 kb on - strand, within CCNA_00327 at 339.925 kb on + strand, within CCNA_00327 at 339.941 kb on - strand, within CCNA_00327 at 339.953 kb on + strand, within CCNA_00327 at 339.982 kb on - strand, within CCNA_00327 at 339.982 kb on - strand, within CCNA_00327 at 339.982 kb on - strand, within CCNA_00327 at 339.997 kb on - strand, within CCNA_00327 at 340.006 kb on - strand, within CCNA_00327 at 340.008 kb on + strand, within CCNA_00327 at 340.008 kb on + strand, within CCNA_00327 at 340.008 kb on + strand, within CCNA_00327 at 340.044 kb on + strand, within CCNA_00327 at 340.045 kb on - strand, within CCNA_00327 at 340.053 kb on - strand, within CCNA_00327 at 340.053 kb on - strand, within CCNA_00327 at 340.066 kb on - strand, within CCNA_00327 at 340.074 kb on - strand, within CCNA_00327 at 340.079 kb on - strand, within CCNA_00327 at 340.131 kb on + strand, within CCNA_00327 at 340.169 kb on + strand, within CCNA_00327 at 340.177 kb on - strand, within CCNA_00327 at 340.177 kb on - strand, within CCNA_00327 at 340.177 kb on - strand, within CCNA_00327 at 340.194 kb on - strand, within CCNA_00327 at 340.198 kb on + strand, within CCNA_00327 at 340.200 kb on + strand, within CCNA_00327 at 340.200 kb on + strand, within CCNA_00327 at 340.206 kb on - strand, within CCNA_00327 at 340.206 kb on - strand, within CCNA_00327 at 340.206 kb on - strand, within CCNA_00327 at 340.225 kb on - strand, within CCNA_00327 at 340.243 kb on + strand, within CCNA_00327 at 340.251 kb on - strand, within CCNA_00327 at 340.251 kb on - strand, within CCNA_00327 at 340.272 kb on - strand, within CCNA_00327 at 340.274 kb on + strand, within CCNA_00327 at 340.280 kb on + strand, within CCNA_00327 at 340.288 kb on - strand, within CCNA_00327 at 340.291 kb on + strand, within CCNA_00327 at 340.292 kb on - strand, within CCNA_00327 at 340.338 kb on + strand, within CCNA_00327 at 340.353 kb on - strand, within CCNA_00327 at 340.353 kb on - strand, within CCNA_00327 at 340.353 kb on - strand, within CCNA_00327 at 340.359 kb on + strand, within CCNA_00327 at 340.396 kb on + strand, within CCNA_00327 at 340.396 kb on + strand, within CCNA_00327 at 340.418 kb on + strand, within CCNA_00327 at 340.426 kb on - strand at 340.475 kb on - strand at 340.486 kb on + strand at 340.507 kb on - strand at 340.521 kb on - strand at 340.521 kb on - strand at 340.545 kb on + strand at 340.554 kb on - strand at 340.572 kb on + strand at 340.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Benzalkonium Chloride 0.0001 mg/ml remove 337,644 - +0.7 337,663 + +2.2 337,671 - -0.1 337,688 + +2.9 337,731 + +1.7 337,732 + +2.0 337,747 - +0.2 337,759 + +1.8 337,779 - +1.7 337,795 + +1.8 337,796 + +2.9 337,803 + +2.3 337,803 + +2.2 337,811 - +1.0 337,816 - +0.8 337,830 + -0.4 337,837 + +2.1 337,842 + +2.2 337,842 + +2.0 337,842 + +2.0 337,842 + +1.8 337,850 - -0.8 337,850 - +0.8 337,850 - +1.1 337,891 - +3.2 337,961 + +0.4 337,963 - +3.8 337,963 - +3.8 337,999 + CCNA_00324 0.15 +1.6 337,999 + CCNA_00324 0.15 +2.1 338,011 + CCNA_00324 0.17 +0.1 338,228 + CCNA_00324 0.64 -0.6 338,244 + CCNA_00324 0.68 +0.1 338,252 - CCNA_00324 0.70 +3.1 338,261 - CCNA_00324 0.72 +3.6 338,309 + CCNA_00324 0.82 -0.4 338,382 + -1.2 338,385 - +2.3 338,385 - +2.3 338,390 - +1.7 338,390 - +2.1 338,400 - +5.8 338,452 + -2.5 338,455 - +3.0 338,460 - +2.1 338,603 - -1.0 338,604 - -1.8 338,604 - -1.3 338,618 + -1.5 338,618 + -1.2 338,618 + -2.1 338,618 + -0.4 338,618 + -1.6 338,626 - +0.2 338,626 - -0.0 338,626 - -1.1 338,731 - +1.6 338,741 - CCNA_00326 0.10 -1.0 338,759 - CCNA_00326 0.12 -0.9 338,784 + CCNA_00326 0.15 +0.9 338,792 - CCNA_00326 0.15 +0.2 338,804 + CCNA_00326 0.16 -0.3 338,850 + CCNA_00326 0.21 +1.8 338,881 + CCNA_00326 0.24 -0.1 338,941 + CCNA_00326 0.30 -1.3 338,946 + CCNA_00326 0.30 +0.0 338,997 + CCNA_00326 0.35 +0.9 339,005 - CCNA_00326 0.36 +0.4 339,016 + CCNA_00326 0.37 +2.5 339,042 + CCNA_00326 0.40 +0.5 339,046 - CCNA_00326 0.40 +0.4 339,050 - CCNA_00326 0.40 +2.1 339,096 + CCNA_00326 0.45 -0.0 339,138 + CCNA_00326 0.49 -0.3 339,138 + CCNA_00326 0.49 -0.2 339,138 + CCNA_00326 0.49 +1.5 339,138 + CCNA_00326 0.49 +1.0 339,138 + CCNA_00326 0.49 +0.9 339,138 + CCNA_00326 0.49 +1.4 339,138 + CCNA_00326 0.49 +2.4 339,146 - CCNA_00326 0.50 -0.1 339,146 - CCNA_00326 0.50 +0.2 339,146 - CCNA_00326 0.50 +0.6 339,191 + CCNA_00326 0.54 -0.3 339,289 + CCNA_00326 0.63 +0.8 339,308 + CCNA_00326 0.65 +0.5 339,308 + CCNA_00326 0.65 +0.8 339,317 - CCNA_00326 0.66 -1.5 339,327 + CCNA_00326 0.67 +1.0 339,347 + CCNA_00326 0.69 +1.2 339,347 + CCNA_00326 0.69 +0.9 339,355 - CCNA_00326 0.70 -0.7 339,415 + CCNA_00326 0.76 +0.6 339,423 - CCNA_00326 0.76 -1.0 339,468 + CCNA_00326 0.81 +1.0 339,468 + CCNA_00326 0.81 +1.2 339,485 + CCNA_00326 0.82 +1.4 339,485 + CCNA_00326 0.82 +1.6 339,485 + CCNA_00326 0.82 +1.7 339,488 - CCNA_00326 0.83 -2.5 339,493 - CCNA_00326 0.83 -0.8 339,493 - CCNA_00326 0.83 +0.9 339,501 - CCNA_00326 0.84 -0.8 339,524 + CCNA_00326 0.86 -0.4 339,531 + CCNA_00326 0.87 +1.2 339,532 - CCNA_00326 0.87 -1.7 339,532 - CCNA_00326 0.87 +0.6 339,539 - CCNA_00326 0.88 +0.1 339,590 - +0.8 339,626 - +0.2 339,629 - +0.2 339,660 + +0.8 339,661 - +0.0 339,681 + +0.5 339,691 + +1.0 339,699 - -2.1 339,735 + +1.2 339,769 + -1.1 339,769 + +1.2 339,791 + -0.6 339,806 + +0.3 339,806 + -0.1 339,806 + -1.0 339,814 - +0.5 339,816 + +0.8 339,875 + -1.2 339,915 - CCNA_00327 0.13 +0.1 339,925 + CCNA_00327 0.15 +1.2 339,941 - CCNA_00327 0.17 +0.2 339,953 + CCNA_00327 0.19 -1.0 339,982 - CCNA_00327 0.23 -0.8 339,982 - CCNA_00327 0.23 -2.5 339,982 - CCNA_00327 0.23 +0.6 339,997 - CCNA_00327 0.25 -0.8 340,006 - CCNA_00327 0.27 -1.2 340,008 + CCNA_00327 0.27 -1.4 340,008 + CCNA_00327 0.27 -1.3 340,008 + CCNA_00327 0.27 -2.2 340,044 + CCNA_00327 0.33 -0.5 340,045 - CCNA_00327 0.33 -1.6 340,053 - CCNA_00327 0.34 -0.1 340,053 - CCNA_00327 0.34 -1.3 340,066 - CCNA_00327 0.36 -1.4 340,074 - CCNA_00327 0.37 -0.8 340,079 - CCNA_00327 0.38 -0.4 340,131 + CCNA_00327 0.46 -0.3 340,169 + CCNA_00327 0.51 -1.5 340,177 - CCNA_00327 0.53 -0.5 340,177 - CCNA_00327 0.53 +0.9 340,177 - CCNA_00327 0.53 -0.0 340,194 - CCNA_00327 0.55 -0.9 340,198 + CCNA_00327 0.56 -0.8 340,200 + CCNA_00327 0.56 -1.2 340,200 + CCNA_00327 0.56 +0.2 340,206 - CCNA_00327 0.57 +1.3 340,206 - CCNA_00327 0.57 -0.0 340,206 - CCNA_00327 0.57 -0.3 340,225 - CCNA_00327 0.60 -0.0 340,243 + CCNA_00327 0.63 -1.2 340,251 - CCNA_00327 0.64 +0.8 340,251 - CCNA_00327 0.64 -0.3 340,272 - CCNA_00327 0.67 +2.4 340,274 + CCNA_00327 0.67 +0.2 340,280 + CCNA_00327 0.68 -1.7 340,288 - CCNA_00327 0.69 -0.4 340,291 + CCNA_00327 0.70 -0.7 340,292 - CCNA_00327 0.70 -0.6 340,338 + CCNA_00327 0.77 -1.9 340,353 - CCNA_00327 0.79 -1.1 340,353 - CCNA_00327 0.79 -2.3 340,353 - CCNA_00327 0.79 -3.0 340,359 + CCNA_00327 0.80 -0.8 340,396 + CCNA_00327 0.86 -0.4 340,396 + CCNA_00327 0.86 -0.9 340,418 + CCNA_00327 0.89 +1.0 340,426 - -0.4 340,475 - +1.6 340,486 + +0.1 340,507 - +0.1 340,521 - +1.4 340,521 - +0.5 340,545 + +0.8 340,554 - -0.6 340,572 + -0.5 340,614 - -0.5
Or see this region's nucleotide sequence