Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00117

Experiment: PYE with Benzalkonium Chloride 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00116 and CCNA_00117 are separated by 161 nucleotidesCCNA_00117 and CCNA_00118 are separated by 10 nucleotides CCNA_00116: CCNA_00116 - phosphoglucosamine mutase, at 126,407 to 127,753 _00116 CCNA_00117: CCNA_00117 - glucosamine-fructose-6-phosphate aminotransferase, isomerizing, at 127,915 to 129,735 _00117 CCNA_00118: CCNA_00118 - porphyrin biosynthesis protein, at 129,746 to 131,989 _00118 Position (kb) 127 128 129 130Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 127.770 kb on + strandat 127.778 kb on - strandat 127.778 kb on - strandat 127.837 kb on + strandat 127.846 kb on - strandat 127.883 kb on - strandat 127.883 kb on - strandat 129.727 kb on + strandat 129.773 kb on + strandat 129.780 kb on - strandat 129.795 kb on + strandat 129.836 kb on + strandat 129.836 kb on + strandat 129.844 kb on - strandat 129.954 kb on - strandat 129.970 kb on - strandat 129.976 kb on - strand, within CCNA_00118at 130.004 kb on + strand, within CCNA_00118at 130.029 kb on + strand, within CCNA_00118at 130.029 kb on + strand, within CCNA_00118at 130.045 kb on + strand, within CCNA_00118at 130.121 kb on + strand, within CCNA_00118at 130.121 kb on + strand, within CCNA_00118at 130.152 kb on - strand, within CCNA_00118at 130.152 kb on - strand, within CCNA_00118at 130.152 kb on - strand, within CCNA_00118at 130.166 kb on - strand, within CCNA_00118at 130.169 kb on + strand, within CCNA_00118at 130.169 kb on + strand, within CCNA_00118at 130.181 kb on + strand, within CCNA_00118at 130.182 kb on + strand, within CCNA_00118at 130.182 kb on + strand, within CCNA_00118at 130.183 kb on - strand, within CCNA_00118at 130.189 kb on - strand, within CCNA_00118at 130.209 kb on + strand, within CCNA_00118at 130.221 kb on + strand, within CCNA_00118at 130.249 kb on - strand, within CCNA_00118at 130.260 kb on + strand, within CCNA_00118at 130.300 kb on - strand, within CCNA_00118at 130.301 kb on - strand, within CCNA_00118at 130.307 kb on - strand, within CCNA_00118at 130.338 kb on + strand, within CCNA_00118at 130.355 kb on + strand, within CCNA_00118at 130.363 kb on + strand, within CCNA_00118at 130.363 kb on + strand, within CCNA_00118at 130.399 kb on + strand, within CCNA_00118at 130.406 kb on + strand, within CCNA_00118at 130.407 kb on + strand, within CCNA_00118at 130.414 kb on - strand, within CCNA_00118at 130.414 kb on - strand, within CCNA_00118at 130.418 kb on + strand, within CCNA_00118at 130.424 kb on - strand, within CCNA_00118at 130.426 kb on - strand, within CCNA_00118at 130.428 kb on + strand, within CCNA_00118at 130.428 kb on + strand, within CCNA_00118at 130.428 kb on + strand, within CCNA_00118at 130.428 kb on + strand, within CCNA_00118at 130.436 kb on - strand, within CCNA_00118at 130.436 kb on - strand, within CCNA_00118at 130.436 kb on - strand, within CCNA_00118at 130.436 kb on - strand, within CCNA_00118at 130.442 kb on - strand, within CCNA_00118at 130.445 kb on + strand, within CCNA_00118at 130.453 kb on - strand, within CCNA_00118at 130.453 kb on - strand, within CCNA_00118at 130.493 kb on - strand, within CCNA_00118at 130.526 kb on - strand, within CCNA_00118at 130.526 kb on - strand, within CCNA_00118at 130.558 kb on + strand, within CCNA_00118at 130.558 kb on - strand, within CCNA_00118at 130.558 kb on - strand, within CCNA_00118at 130.573 kb on - strand, within CCNA_00118at 130.595 kb on - strand, within CCNA_00118at 130.633 kb on + strand, within CCNA_00118at 130.659 kb on - strand, within CCNA_00118at 130.693 kb on - strand, within CCNA_00118at 130.693 kb on - strand, within CCNA_00118at 130.723 kb on + strand, within CCNA_00118

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Benzalkonium Chloride 0.0001 mg/ml
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127,770 + -0.9
127,778 - +0.1
127,778 - -0.1
127,837 + -0.0
127,846 - +1.9
127,883 - -0.9
127,883 - +0.3
129,727 + +0.9
129,773 + -1.1
129,780 - -1.5
129,795 + -2.0
129,836 + -0.2
129,836 + -1.3
129,844 - -1.1
129,954 - +0.5
129,970 - -1.2
129,976 - CCNA_00118 0.10 -0.6
130,004 + CCNA_00118 0.11 -0.6
130,029 + CCNA_00118 0.13 -1.0
130,029 + CCNA_00118 0.13 +0.5
130,045 + CCNA_00118 0.13 -2.4
130,121 + CCNA_00118 0.17 -1.4
130,121 + CCNA_00118 0.17 -0.7
130,152 - CCNA_00118 0.18 -1.9
130,152 - CCNA_00118 0.18 -1.8
130,152 - CCNA_00118 0.18 -1.4
130,166 - CCNA_00118 0.19 -2.2
130,169 + CCNA_00118 0.19 -1.5
130,169 + CCNA_00118 0.19 +0.0
130,181 + CCNA_00118 0.19 +0.8
130,182 + CCNA_00118 0.19 -0.2
130,182 + CCNA_00118 0.19 -1.6
130,183 - CCNA_00118 0.19 -1.5
130,189 - CCNA_00118 0.20 -0.9
130,209 + CCNA_00118 0.21 -0.1
130,221 + CCNA_00118 0.21 +3.4
130,249 - CCNA_00118 0.22 -0.6
130,260 + CCNA_00118 0.23 -1.1
130,300 - CCNA_00118 0.25 +0.3
130,301 - CCNA_00118 0.25 +1.7
130,307 - CCNA_00118 0.25 -2.8
130,338 + CCNA_00118 0.26 -1.1
130,355 + CCNA_00118 0.27 +1.2
130,363 + CCNA_00118 0.27 -1.0
130,363 + CCNA_00118 0.27 +0.9
130,399 + CCNA_00118 0.29 -1.3
130,406 + CCNA_00118 0.29 -0.9
130,407 + CCNA_00118 0.29 -1.6
130,414 - CCNA_00118 0.30 -3.2
130,414 - CCNA_00118 0.30 -1.9
130,418 + CCNA_00118 0.30 -2.3
130,424 - CCNA_00118 0.30 +1.0
130,426 - CCNA_00118 0.30 -1.1
130,428 + CCNA_00118 0.30 -0.3
130,428 + CCNA_00118 0.30 -4.1
130,428 + CCNA_00118 0.30 -0.8
130,428 + CCNA_00118 0.30 +0.7
130,436 - CCNA_00118 0.31 -0.4
130,436 - CCNA_00118 0.31 +0.0
130,436 - CCNA_00118 0.31 -0.9
130,436 - CCNA_00118 0.31 -0.2
130,442 - CCNA_00118 0.31 -3.0
130,445 + CCNA_00118 0.31 -0.7
130,453 - CCNA_00118 0.32 +0.3
130,453 - CCNA_00118 0.32 -0.9
130,493 - CCNA_00118 0.33 -0.9
130,526 - CCNA_00118 0.35 +3.1
130,526 - CCNA_00118 0.35 +2.9
130,558 + CCNA_00118 0.36 -0.4
130,558 - CCNA_00118 0.36 -2.7
130,558 - CCNA_00118 0.36 -1.5
130,573 - CCNA_00118 0.37 -0.8
130,595 - CCNA_00118 0.38 +0.0
130,633 + CCNA_00118 0.40 -1.3
130,659 - CCNA_00118 0.41 -1.0
130,693 - CCNA_00118 0.42 +0.2
130,693 - CCNA_00118 0.42 -1.2
130,723 + CCNA_00118 0.44 +0.8

Or see this region's nucleotide sequence