Experiment: Amylose from potato
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2615 and BT2616 are separated by 323 nucleotides BT2616 and BT2617 overlap by 25 nucleotides
BT2615: BT2615 - reverse transcriptase (NCBI ptt file), at 3,254,829 to 3,256,121
BT2615
BT2616: BT2616 - hypothetical protein (NCBI ptt file), at 3,256,445 to 3,256,720
BT2616
BT2617: BT2617 - reverse transcriptase (NCBI ptt file), at 3,256,696 to 3,258,102
BT2617
Position (kb)
3256
3257 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3255.496 kb on + strand, within BT2615 at 3255.497 kb on - strand, within BT2615 at 3255.528 kb on + strand at 3255.529 kb on - strand, within BT2615 at 3255.612 kb on + strand, within BT2615 at 3255.612 kb on + strand, within BT2615 at 3255.612 kb on + strand, within BT2615 at 3255.616 kb on + strand, within BT2615 at 3255.616 kb on + strand, within BT2615 at 3255.617 kb on - strand, within BT2615 at 3255.667 kb on - strand, within BT2615 at 3255.667 kb on - strand, within BT2615 at 3255.679 kb on - strand, within BT2615 at 3255.679 kb on - strand, within BT2615 at 3255.791 kb on - strand, within BT2615 at 3255.818 kb on + strand, within BT2615 at 3255.840 kb on + strand, within BT2615 at 3255.894 kb on - strand, within BT2615 at 3255.975 kb on - strand, within BT2615 at 3256.095 kb on + strand at 3256.127 kb on + strand at 3256.144 kb on + strand at 3256.203 kb on + strand at 3256.204 kb on - strand at 3256.208 kb on + strand at 3256.211 kb on + strand at 3256.233 kb on - strand at 3256.234 kb on + strand at 3256.235 kb on - strand at 3256.293 kb on + strand at 3256.295 kb on + strand at 3256.295 kb on + strand at 3256.317 kb on + strand at 3256.317 kb on + strand at 3256.318 kb on - strand at 3256.371 kb on - strand at 3256.371 kb on - strand at 3256.431 kb on + strand at 3256.431 kb on + strand at 3256.432 kb on - strand at 3256.480 kb on - strand, within BT2616 at 3256.483 kb on - strand, within BT2616 at 3256.483 kb on - strand, within BT2616 at 3256.487 kb on + strand, within BT2616 at 3256.488 kb on - strand, within BT2616 at 3256.518 kb on + strand, within BT2616 at 3256.555 kb on + strand, within BT2616 at 3256.555 kb on + strand, within BT2616 at 3256.601 kb on - strand, within BT2616 at 3256.674 kb on - strand, within BT2616 at 3256.697 kb on + strand at 3256.739 kb on + strand at 3256.740 kb on - strand at 3256.781 kb on - strand at 3256.784 kb on - strand at 3256.784 kb on - strand at 3256.835 kb on + strand at 3256.835 kb on + strand at 3256.836 kb on - strand at 3256.877 kb on - strand, within BT2617 at 3256.912 kb on - strand, within BT2617 at 3256.924 kb on - strand, within BT2617 at 3256.953 kb on + strand, within BT2617 at 3256.985 kb on + strand, within BT2617 at 3256.986 kb on - strand, within BT2617 at 3257.006 kb on + strand, within BT2617 at 3257.006 kb on + strand, within BT2617 at 3257.107 kb on + strand, within BT2617 at 3257.108 kb on - strand, within BT2617 at 3257.113 kb on + strand, within BT2617 at 3257.138 kb on + strand, within BT2617 at 3257.161 kb on + strand, within BT2617 at 3257.174 kb on + strand, within BT2617 at 3257.175 kb on - strand, within BT2617 at 3257.179 kb on + strand, within BT2617 at 3257.224 kb on + strand, within BT2617 at 3257.225 kb on - strand, within BT2617 at 3257.236 kb on + strand, within BT2617 at 3257.281 kb on + strand, within BT2617 at 3257.282 kb on - strand, within BT2617 at 3257.359 kb on - strand, within BT2617 at 3257.365 kb on + strand, within BT2617 at 3257.450 kb on + strand, within BT2617 at 3257.459 kb on - strand, within BT2617 at 3257.471 kb on - strand, within BT2617 at 3257.484 kb on - strand, within BT2617 at 3257.486 kb on - strand, within BT2617 at 3257.500 kb on + strand, within BT2617 at 3257.500 kb on + strand, within BT2617 at 3257.517 kb on + strand, within BT2617 at 3257.560 kb on + strand, within BT2617 at 3257.564 kb on + strand, within BT2617 at 3257.564 kb on + strand, within BT2617 at 3257.566 kb on + strand, within BT2617 at 3257.566 kb on + strand, within BT2617 at 3257.567 kb on - strand, within BT2617 at 3257.567 kb on - strand, within BT2617 at 3257.567 kb on - strand, within BT2617 at 3257.568 kb on + strand, within BT2617 at 3257.568 kb on + strand, within BT2617 at 3257.568 kb on + strand, within BT2617 at 3257.569 kb on - strand, within BT2617 at 3257.569 kb on - strand, within BT2617 at 3257.569 kb on - strand, within BT2617 at 3257.570 kb on + strand, within BT2617 at 3257.571 kb on - strand, within BT2617 at 3257.571 kb on - strand, within BT2617 at 3257.667 kb on - strand, within BT2617 at 3257.667 kb on - strand, within BT2617
Per-strain Table
Position Strand Gene LocusTag Fraction Amylose from potato remove 3,255,496 + BT2615 0.52 -1.4 3,255,497 - BT2615 0.52 -1.0 3,255,528 + -0.0 3,255,529 - BT2615 0.54 -2.0 3,255,612 + BT2615 0.61 -1.0 3,255,612 + BT2615 0.61 -1.9 3,255,612 + BT2615 0.61 -1.2 3,255,616 + BT2615 0.61 -1.5 3,255,616 + BT2615 0.61 -2.5 3,255,617 - BT2615 0.61 -1.0 3,255,667 - BT2615 0.65 -0.8 3,255,667 - BT2615 0.65 -1.0 3,255,679 - BT2615 0.66 -0.5 3,255,679 - BT2615 0.66 -3.5 3,255,791 - BT2615 0.74 -1.0 3,255,818 + BT2615 0.76 -1.3 3,255,840 + BT2615 0.78 -0.0 3,255,894 - BT2615 0.82 -1.6 3,255,975 - BT2615 0.89 -0.0 3,256,095 + +1.0 3,256,127 + -2.0 3,256,144 + -0.5 3,256,203 + -1.5 3,256,204 - -0.9 3,256,208 + -0.9 3,256,211 + -0.2 3,256,233 - -1.8 3,256,234 + -1.4 3,256,235 - +0.0 3,256,293 + -0.5 3,256,295 + -2.9 3,256,295 + -0.1 3,256,317 + -1.6 3,256,317 + -0.5 3,256,318 - -1.0 3,256,371 - +0.8 3,256,371 - -1.1 3,256,431 + -1.1 3,256,431 + -1.7 3,256,432 - +0.1 3,256,480 - BT2616 0.13 -1.2 3,256,483 - BT2616 0.14 +0.6 3,256,483 - BT2616 0.14 -0.8 3,256,487 + BT2616 0.15 -1.5 3,256,488 - BT2616 0.16 -1.1 3,256,518 + BT2616 0.26 -1.1 3,256,555 + BT2616 0.40 -0.2 3,256,555 + BT2616 0.40 +0.5 3,256,601 - BT2616 0.57 +0.5 3,256,674 - BT2616 0.83 -0.9 3,256,697 + -0.8 3,256,739 + -1.0 3,256,740 - -0.3 3,256,781 - +2.0 3,256,784 - -1.1 3,256,784 - -0.8 3,256,835 + -0.8 3,256,835 + +1.1 3,256,836 - -0.9 3,256,877 - BT2617 0.13 -1.2 3,256,912 - BT2617 0.15 +0.1 3,256,924 - BT2617 0.16 -1.3 3,256,953 + BT2617 0.18 -0.5 3,256,985 + BT2617 0.21 -0.7 3,256,986 - BT2617 0.21 -2.0 3,257,006 + BT2617 0.22 -3.0 3,257,006 + BT2617 0.22 -0.0 3,257,107 + BT2617 0.29 -3.3 3,257,108 - BT2617 0.29 -0.2 3,257,113 + BT2617 0.30 -1.0 3,257,138 + BT2617 0.31 -1.6 3,257,161 + BT2617 0.33 -3.0 3,257,174 + BT2617 0.34 +0.5 3,257,175 - BT2617 0.34 -1.6 3,257,179 + BT2617 0.34 +0.1 3,257,224 + BT2617 0.38 -1.7 3,257,225 - BT2617 0.38 -2.1 3,257,236 + BT2617 0.38 -2.6 3,257,281 + BT2617 0.42 -0.8 3,257,282 - BT2617 0.42 -1.4 3,257,359 - BT2617 0.47 -3.0 3,257,365 + BT2617 0.48 -1.3 3,257,450 + BT2617 0.54 -0.9 3,257,459 - BT2617 0.54 -0.9 3,257,471 - BT2617 0.55 -0.0 3,257,484 - BT2617 0.56 -0.3 3,257,486 - BT2617 0.56 -2.9 3,257,500 + BT2617 0.57 -1.6 3,257,500 + BT2617 0.57 +0.5 3,257,517 + BT2617 0.58 -0.0 3,257,560 + BT2617 0.61 -1.8 3,257,564 + BT2617 0.62 -1.2 3,257,564 + BT2617 0.62 -1.4 3,257,566 + BT2617 0.62 -0.9 3,257,566 + BT2617 0.62 -0.8 3,257,567 - BT2617 0.62 -0.3 3,257,567 - BT2617 0.62 -0.2 3,257,567 - BT2617 0.62 -0.2 3,257,568 + BT2617 0.62 -1.0 3,257,568 + BT2617 0.62 -1.8 3,257,568 + BT2617 0.62 +0.3 3,257,569 - BT2617 0.62 +0.1 3,257,569 - BT2617 0.62 -1.5 3,257,569 - BT2617 0.62 -0.2 3,257,570 + BT2617 0.62 -1.2 3,257,571 - BT2617 0.62 -1.2 3,257,571 - BT2617 0.62 +0.5 3,257,667 - BT2617 0.69 -1.0 3,257,667 - BT2617 0.69 -0.1
Or see this region's nucleotide sequence