Experiment: Amylose from potato
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1445 and BT1446 overlap by 4 nucleotides BT1446 and BT1447 are separated by 24 nucleotides BT1447 and BT1448 are separated by 91 nucleotides BT1448 and BT1449 are separated by 65 nucleotides
BT1445: BT1445 - putative biotin synthesis protein bioC (NCBI ptt file), at 1,779,703 to 1,780,488
BT1445
BT1446: BT1446 - dethiobiotin synthetase (NCBI ptt file), at 1,780,485 to 1,781,132
BT1446
BT1447: BT1447 - two-component system sensor histidine kinase (NCBI ptt file), at 1,781,157 to 1,783,514
BT1447
BT1448: BT1448 - biotin carboxyl carrier protein (NCBI ptt file), at 1,783,606 to 1,784,130
BT1448
BT1449: BT1449 - biotin carboxylase (NCBI ptt file), at 1,784,196 to 1,785,707
BT1449
Position (kb)
1781
1782
1783
1784 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1780.187 kb on - strand, within BT1445 at 1780.234 kb on - strand, within BT1445 at 1780.234 kb on - strand, within BT1445 at 1780.235 kb on + strand, within BT1445 at 1780.447 kb on + strand at 1780.448 kb on - strand at 1780.448 kb on - strand at 1780.448 kb on - strand at 1780.460 kb on - strand at 1780.460 kb on - strand at 1780.467 kb on - strand at 1780.467 kb on - strand at 1780.485 kb on - strand at 1780.639 kb on + strand, within BT1446 at 1780.639 kb on + strand, within BT1446 at 1780.640 kb on - strand, within BT1446 at 1780.640 kb on - strand, within BT1446 at 1780.791 kb on - strand, within BT1446 at 1780.884 kb on + strand, within BT1446 at 1780.885 kb on - strand, within BT1446 at 1780.933 kb on + strand, within BT1446 at 1780.933 kb on + strand, within BT1446 at 1780.934 kb on - strand, within BT1446 at 1780.997 kb on + strand, within BT1446 at 1780.997 kb on + strand, within BT1446 at 1781.003 kb on - strand, within BT1446 at 1781.009 kb on + strand, within BT1446 at 1781.034 kb on - strand, within BT1446 at 1781.051 kb on + strand, within BT1446 at 1781.064 kb on - strand, within BT1446 at 1781.064 kb on - strand, within BT1446 at 1781.139 kb on + strand at 1781.139 kb on + strand at 1781.140 kb on - strand at 1781.141 kb on + strand at 1781.142 kb on - strand at 1781.156 kb on - strand at 1781.454 kb on - strand, within BT1447 at 1781.467 kb on - strand, within BT1447 at 1781.573 kb on + strand, within BT1447 at 1781.597 kb on - strand, within BT1447 at 1781.661 kb on + strand, within BT1447 at 1781.670 kb on + strand, within BT1447 at 1781.783 kb on + strand, within BT1447 at 1781.783 kb on + strand, within BT1447 at 1781.822 kb on + strand, within BT1447 at 1781.880 kb on - strand, within BT1447 at 1781.951 kb on - strand, within BT1447 at 1781.979 kb on - strand, within BT1447 at 1782.054 kb on + strand, within BT1447 at 1782.060 kb on - strand, within BT1447 at 1782.116 kb on + strand, within BT1447 at 1782.116 kb on + strand, within BT1447 at 1782.127 kb on + strand, within BT1447 at 1782.218 kb on + strand, within BT1447 at 1782.218 kb on + strand, within BT1447 at 1782.219 kb on - strand, within BT1447 at 1782.312 kb on - strand, within BT1447 at 1782.409 kb on + strand, within BT1447 at 1782.410 kb on - strand, within BT1447 at 1782.571 kb on + strand, within BT1447 at 1782.577 kb on + strand, within BT1447 at 1782.577 kb on + strand, within BT1447 at 1782.578 kb on - strand, within BT1447 at 1782.608 kb on - strand, within BT1447 at 1782.623 kb on + strand, within BT1447 at 1782.624 kb on - strand, within BT1447 at 1782.717 kb on - strand, within BT1447 at 1782.717 kb on - strand, within BT1447 at 1782.740 kb on + strand, within BT1447 at 1782.746 kb on - strand, within BT1447 at 1782.762 kb on + strand, within BT1447 at 1782.793 kb on + strand, within BT1447 at 1782.805 kb on + strand, within BT1447 at 1782.832 kb on - strand, within BT1447 at 1782.873 kb on - strand, within BT1447 at 1782.889 kb on + strand, within BT1447 at 1782.926 kb on - strand, within BT1447 at 1782.926 kb on - strand, within BT1447 at 1782.953 kb on - strand, within BT1447 at 1782.954 kb on + strand, within BT1447 at 1782.954 kb on + strand, within BT1447 at 1783.020 kb on + strand, within BT1447 at 1783.081 kb on + strand, within BT1447 at 1783.082 kb on - strand, within BT1447 at 1783.118 kb on - strand, within BT1447 at 1783.190 kb on + strand, within BT1447 at 1783.211 kb on - strand, within BT1447 at 1783.359 kb on + strand at 1783.372 kb on + strand at 1783.372 kb on + strand at 1783.412 kb on + strand at 1783.463 kb on + strand at 1783.514 kb on + strand at 1783.529 kb on + strand at 1783.533 kb on + strand at 1783.533 kb on + strand at 1783.534 kb on - strand at 1783.541 kb on + strand at 1783.772 kb on + strand, within BT1448 at 1783.773 kb on - strand, within BT1448 at 1783.849 kb on - strand, within BT1448 at 1783.852 kb on + strand, within BT1448 at 1783.873 kb on + strand, within BT1448 at 1783.874 kb on - strand, within BT1448 at 1783.874 kb on - strand, within BT1448 at 1784.015 kb on - strand, within BT1448 at 1784.070 kb on + strand, within BT1448 at 1784.070 kb on + strand, within BT1448 at 1784.073 kb on - strand, within BT1448 at 1784.089 kb on - strand at 1784.109 kb on - strand at 1784.124 kb on + strand at 1784.125 kb on - strand at 1784.160 kb on + strand at 1784.193 kb on + strand at 1784.204 kb on + strand at 1784.405 kb on - strand, within BT1449 at 1784.481 kb on - strand, within BT1449
Per-strain Table
Position Strand Gene LocusTag Fraction Amylose from potato remove 1,780,187 - BT1445 0.62 -1.0 1,780,234 - BT1445 0.68 -1.7 1,780,234 - BT1445 0.68 -1.7 1,780,235 + BT1445 0.68 -2.0 1,780,447 + -1.3 1,780,448 - -0.3 1,780,448 - -2.9 1,780,448 - -0.9 1,780,460 - -1.5 1,780,460 - -1.2 1,780,467 - -2.2 1,780,467 - -2.3 1,780,485 - -1.5 1,780,639 + BT1446 0.24 +0.4 1,780,639 + BT1446 0.24 -2.1 1,780,640 - BT1446 0.24 -1.6 1,780,640 - BT1446 0.24 -2.2 1,780,791 - BT1446 0.47 -1.9 1,780,884 + BT1446 0.62 -0.7 1,780,885 - BT1446 0.62 +0.1 1,780,933 + BT1446 0.69 -1.7 1,780,933 + BT1446 0.69 -0.2 1,780,934 - BT1446 0.69 -2.5 1,780,997 + BT1446 0.79 -2.0 1,780,997 + BT1446 0.79 +0.9 1,781,003 - BT1446 0.80 +2.2 1,781,009 + BT1446 0.81 -2.4 1,781,034 - BT1446 0.85 -1.7 1,781,051 + BT1446 0.87 -1.2 1,781,064 - BT1446 0.89 -1.6 1,781,064 - BT1446 0.89 -0.7 1,781,139 + +0.7 1,781,139 + +0.7 1,781,140 - -0.5 1,781,141 + +0.5 1,781,142 - -0.2 1,781,156 - -1.5 1,781,454 - BT1447 0.13 +0.3 1,781,467 - BT1447 0.13 -2.2 1,781,573 + BT1447 0.18 -0.9 1,781,597 - BT1447 0.19 -1.5 1,781,661 + BT1447 0.21 -1.8 1,781,670 + BT1447 0.22 -2.4 1,781,783 + BT1447 0.27 -0.6 1,781,783 + BT1447 0.27 -1.3 1,781,822 + BT1447 0.28 -1.2 1,781,880 - BT1447 0.31 -0.2 1,781,951 - BT1447 0.34 -0.6 1,781,979 - BT1447 0.35 -0.3 1,782,054 + BT1447 0.38 -1.2 1,782,060 - BT1447 0.38 -0.7 1,782,116 + BT1447 0.41 +0.4 1,782,116 + BT1447 0.41 -1.0 1,782,127 + BT1447 0.41 -1.2 1,782,218 + BT1447 0.45 -1.5 1,782,218 + BT1447 0.45 -0.7 1,782,219 - BT1447 0.45 -2.3 1,782,312 - BT1447 0.49 -0.2 1,782,409 + BT1447 0.53 -0.7 1,782,410 - BT1447 0.53 -2.3 1,782,571 + BT1447 0.60 -1.4 1,782,577 + BT1447 0.60 -1.9 1,782,577 + BT1447 0.60 -2.1 1,782,578 - BT1447 0.60 -0.2 1,782,608 - BT1447 0.62 -0.9 1,782,623 + BT1447 0.62 -0.7 1,782,624 - BT1447 0.62 +0.1 1,782,717 - BT1447 0.66 -0.3 1,782,717 - BT1447 0.66 +0.4 1,782,740 + BT1447 0.67 -0.0 1,782,746 - BT1447 0.67 -1.5 1,782,762 + BT1447 0.68 -1.5 1,782,793 + BT1447 0.69 -1.0 1,782,805 + BT1447 0.70 -0.5 1,782,832 - BT1447 0.71 +0.7 1,782,873 - BT1447 0.73 -0.7 1,782,889 + BT1447 0.73 -0.7 1,782,926 - BT1447 0.75 -1.5 1,782,926 - BT1447 0.75 -0.7 1,782,953 - BT1447 0.76 -2.1 1,782,954 + BT1447 0.76 -0.9 1,782,954 + BT1447 0.76 -1.5 1,783,020 + BT1447 0.79 -1.5 1,783,081 + BT1447 0.82 -1.5 1,783,082 - BT1447 0.82 -1.7 1,783,118 - BT1447 0.83 +0.9 1,783,190 + BT1447 0.86 -1.9 1,783,211 - BT1447 0.87 -0.3 1,783,359 + -2.3 1,783,372 + -2.0 1,783,372 + -0.9 1,783,412 + -0.7 1,783,463 + +1.2 1,783,514 + -1.1 1,783,529 + -0.3 1,783,533 + -0.7 1,783,533 + -0.3 1,783,534 - -0.7 1,783,541 + -0.5 1,783,772 + BT1448 0.32 -0.5 1,783,773 - BT1448 0.32 -1.0 1,783,849 - BT1448 0.46 -0.9 1,783,852 + BT1448 0.47 -0.9 1,783,873 + BT1448 0.51 -0.2 1,783,874 - BT1448 0.51 +0.5 1,783,874 - BT1448 0.51 -0.3 1,784,015 - BT1448 0.78 +1.7 1,784,070 + BT1448 0.88 -0.9 1,784,070 + BT1448 0.88 -0.7 1,784,073 - BT1448 0.89 -1.4 1,784,089 - -1.3 1,784,109 - -0.7 1,784,124 + -1.3 1,784,125 - -0.9 1,784,160 + -1.5 1,784,193 + -0.3 1,784,204 + -1.0 1,784,405 - BT1449 0.14 -0.3 1,784,481 - BT1449 0.19 -0.1
Or see this region's nucleotide sequence