Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02252 and SMc02253 are separated by 18 nucleotides SMc02253 and SMc02254 are separated by 234 nucleotides SMc02254 and SMc02255 overlap by 11 nucleotides
SMc02252: SMc02252 - UDP-glucose 4-epimerase, at 617,107 to 618,135
SMc02252
SMc02253: SMc02253 - salicylate biosynthesis protein, at 618,154 to 618,459
SMc02253
SMc02254: SMc02254 - quinol oxidase subunit II transmembrane protein, at 618,694 to 619,698
SMc02254
SMc02255: SMc02255 - quinol oxidase subunit I transmembrane protein, at 619,688 to 621,169
SMc02255
Position (kb) 618
619
620 Strain fitness (log2 ratio) -2
-1
0
1
2 at 617.743 kb on + strand, within SMc02252 at 617.776 kb on + strand, within SMc02252 at 617.776 kb on + strand, within SMc02252 at 617.776 kb on + strand, within SMc02252 at 617.777 kb on - strand, within SMc02252 at 617.777 kb on - strand, within SMc02252 at 617.778 kb on + strand, within SMc02252 at 617.779 kb on - strand, within SMc02252 at 617.824 kb on + strand, within SMc02252 at 617.824 kb on + strand, within SMc02252 at 617.824 kb on + strand, within SMc02252 at 617.824 kb on + strand, within SMc02252 at 617.828 kb on + strand, within SMc02252 at 617.829 kb on - strand, within SMc02252 at 617.941 kb on + strand, within SMc02252 at 617.975 kb on + strand, within SMc02252 at 617.975 kb on + strand, within SMc02252 at 618.043 kb on - strand at 618.230 kb on + strand, within SMc02253 at 618.230 kb on + strand, within SMc02253 at 618.354 kb on + strand, within SMc02253 at 618.356 kb on + strand, within SMc02253 at 618.357 kb on - strand, within SMc02253 at 618.418 kb on + strand, within SMc02253 at 618.418 kb on + strand, within SMc02253 at 618.419 kb on - strand, within SMc02253 at 618.419 kb on - strand, within SMc02253 at 618.511 kb on - strand at 618.701 kb on + strand at 618.747 kb on - strand at 619.640 kb on + strand at 619.781 kb on - strand at 619.816 kb on - strand at 619.816 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 617,743 + SMc02252 0.62 -0.6 617,776 + SMc02252 0.65 +0.1 617,776 + SMc02252 0.65 +0.3 617,776 + SMc02252 0.65 +0.6 617,777 - SMc02252 0.65 -0.6 617,777 - SMc02252 0.65 +0.8 617,778 + SMc02252 0.65 +1.0 617,779 - SMc02252 0.65 +1.5 617,824 + SMc02252 0.70 -2.5 617,824 + SMc02252 0.70 -0.5 617,824 + SMc02252 0.70 +2.0 617,824 + SMc02252 0.70 -0.5 617,828 + SMc02252 0.70 -0.6 617,829 - SMc02252 0.70 -0.1 617,941 + SMc02252 0.81 +0.2 617,975 + SMc02252 0.84 -0.3 617,975 + SMc02252 0.84 +0.2 618,043 - -0.0 618,230 + SMc02253 0.25 +0.6 618,230 + SMc02253 0.25 +0.2 618,354 + SMc02253 0.65 -1.0 618,356 + SMc02253 0.66 -1.0 618,357 - SMc02253 0.66 -1.5 618,418 + SMc02253 0.86 -0.0 618,418 + SMc02253 0.86 +0.9 618,419 - SMc02253 0.87 -0.4 618,419 - SMc02253 0.87 +0.9 618,511 - -0.1 618,701 + +0.7 618,747 - -0.7 619,640 + +0.6 619,781 - +1.2 619,816 - +0.6 619,816 - +0.9
Or see this region's nucleotide sequence