Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01802 and SMc01803 are separated by 154 nucleotides SMc01803 and SMc01804 are separated by 2 nucleotides SMc01804 and SMc01805 are separated by 218 nucleotides SMc01805 and SMc01806 are separated by 168 nucleotides
SMc01802: SMc01802 - agmatinase, at 1,349,168 to 1,350,124
SMc01802
SMc01803: SMc01803 - 30S ribosomal protein S9, at 1,350,279 to 1,350,746
SMc01803
SMc01804: SMc01804 - 50S ribosomal protein L13, at 1,350,749 to 1,351,213
SMc01804
SMc01805: SMc01805 - hypothetical protein, at 1,351,432 to 1,351,863
SMc01805
SMc01806: SMc01806 - enoyl-CoA hydratase, at 1,352,032 to 1,352,853
SMc01806
Position (kb)
1350
1351
1352 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1349.749 kb on - strand, within SMc01802 at 1349.818 kb on - strand, within SMc01802 at 1350.021 kb on + strand, within SMc01802 at 1351.498 kb on + strand, within SMc01805 at 1351.551 kb on + strand, within SMc01805 at 1351.551 kb on + strand, within SMc01805 at 1351.551 kb on + strand, within SMc01805 at 1351.552 kb on - strand, within SMc01805 at 1351.633 kb on - strand, within SMc01805 at 1351.651 kb on - strand, within SMc01805 at 1351.653 kb on - strand, within SMc01805 at 1351.879 kb on + strand at 1351.879 kb on + strand at 1351.879 kb on + strand at 1351.879 kb on + strand at 1351.880 kb on - strand at 1351.901 kb on + strand at 1351.943 kb on + strand at 1351.943 kb on + strand at 1351.943 kb on + strand at 1351.944 kb on - strand at 1351.944 kb on - strand at 1351.944 kb on - strand at 1351.944 kb on - strand at 1352.113 kb on - strand at 1352.113 kb on - strand at 1352.113 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 1,349,749 - SMc01802 0.61 -1.6 1,349,818 - SMc01802 0.68 -0.7 1,350,021 + SMc01802 0.89 -1.8 1,351,498 + SMc01805 0.15 +0.3 1,351,551 + SMc01805 0.28 +0.0 1,351,551 + SMc01805 0.28 +0.3 1,351,551 + SMc01805 0.28 +0.1 1,351,552 - SMc01805 0.28 +0.3 1,351,633 - SMc01805 0.47 +2.0 1,351,651 - SMc01805 0.51 +2.4 1,351,653 - SMc01805 0.51 +0.4 1,351,879 + +0.2 1,351,879 + +0.9 1,351,879 + +1.2 1,351,879 + +0.7 1,351,880 - -3.0 1,351,901 + -2.4 1,351,943 + +1.1 1,351,943 + -0.1 1,351,943 + +0.1 1,351,944 - -0.1 1,351,944 - +0.7 1,351,944 - +0.2 1,351,944 - +0.8 1,352,113 - -0.4 1,352,113 - -1.2 1,352,113 - -0.1
Or see this region's nucleotide sequence