Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1525 and SMa1526 are separated by 3 nucleotides SMa1526 and SMa1529 are separated by 18 nucleotides SMa1529 and SMa1531 are separated by 16 nucleotides SMa1531 and SMa1532 overlap by 4 nucleotides
SMa1525: SMa1525 - NuoF2 NADH I chain F, at 841,280 to 842,545
SMa1525
SMa1526: SMa1526 - NuoE2 NADH I chain E, at 842,549 to 843,055
SMa1526
SMa1529: SMa1529 - NuoD2 NADH I chain D, at 843,074 to 844,288
SMa1529
SMa1531: SMa1531 - NuoC2 NADH I chain C, at 844,305 to 844,853
SMa1531
SMa1532: SMa1532 - NADH dehydrogenase subunit B, at 844,850 to 845,353
SMa1532
Position (kb) 843
844
845 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 842.153 kb on + strand, within SMa1525 at 842.166 kb on + strand, within SMa1525 at 842.167 kb on - strand, within SMa1525 at 842.167 kb on - strand, within SMa1525 at 842.182 kb on - strand, within SMa1525 at 842.182 kb on - strand, within SMa1525 at 842.187 kb on + strand, within SMa1525 at 842.188 kb on - strand, within SMa1525 at 842.188 kb on - strand, within SMa1525 at 842.188 kb on - strand, within SMa1525 at 842.188 kb on - strand, within SMa1525 at 842.211 kb on + strand, within SMa1525 at 842.211 kb on + strand, within SMa1525 at 842.316 kb on + strand, within SMa1525 at 842.317 kb on - strand, within SMa1525 at 842.317 kb on - strand, within SMa1525 at 842.434 kb on + strand at 842.434 kb on + strand at 842.550 kb on + strand at 842.550 kb on + strand at 842.675 kb on - strand, within SMa1526 at 842.874 kb on + strand, within SMa1526 at 842.892 kb on + strand, within SMa1526 at 843.075 kb on + strand at 843.138 kb on + strand at 843.139 kb on - strand at 843.238 kb on - strand, within SMa1529 at 843.238 kb on - strand, within SMa1529 at 843.238 kb on - strand, within SMa1529 at 843.238 kb on - strand, within SMa1529 at 843.277 kb on - strand, within SMa1529 at 843.391 kb on + strand, within SMa1529 at 843.538 kb on - strand, within SMa1529 at 843.538 kb on - strand, within SMa1529 at 843.655 kb on + strand, within SMa1529 at 843.684 kb on - strand, within SMa1529 at 843.696 kb on + strand, within SMa1529 at 843.719 kb on + strand, within SMa1529 at 843.793 kb on - strand, within SMa1529 at 843.865 kb on - strand, within SMa1529 at 843.895 kb on + strand, within SMa1529 at 843.961 kb on + strand, within SMa1529 at 843.963 kb on + strand, within SMa1529 at 843.964 kb on - strand, within SMa1529 at 843.987 kb on - strand, within SMa1529 at 844.047 kb on + strand, within SMa1529 at 844.125 kb on + strand, within SMa1529 at 844.133 kb on - strand, within SMa1529 at 844.156 kb on - strand, within SMa1529 at 844.156 kb on - strand, within SMa1529 at 844.323 kb on + strand at 844.324 kb on - strand at 844.325 kb on + strand at 844.326 kb on - strand at 844.378 kb on - strand, within SMa1531 at 844.397 kb on - strand, within SMa1531 at 844.453 kb on + strand, within SMa1531 at 844.453 kb on + strand, within SMa1531 at 844.453 kb on + strand, within SMa1531 at 844.495 kb on + strand, within SMa1531 at 844.495 kb on + strand, within SMa1531 at 844.495 kb on + strand, within SMa1531 at 844.495 kb on + strand, within SMa1531 at 844.496 kb on - strand, within SMa1531 at 844.496 kb on - strand, within SMa1531 at 844.500 kb on - strand, within SMa1531 at 844.500 kb on - strand, within SMa1531 at 844.618 kb on + strand, within SMa1531 at 844.618 kb on + strand, within SMa1531 at 844.618 kb on + strand, within SMa1531 at 844.627 kb on + strand, within SMa1531 at 844.627 kb on + strand, within SMa1531 at 844.628 kb on - strand, within SMa1531 at 844.674 kb on + strand, within SMa1531 at 844.674 kb on + strand, within SMa1531 at 844.675 kb on - strand, within SMa1531 at 844.675 kb on - strand, within SMa1531 at 844.765 kb on + strand, within SMa1531 at 844.765 kb on + strand, within SMa1531 at 844.765 kb on + strand, within SMa1531 at 844.766 kb on - strand, within SMa1531 at 844.766 kb on - strand, within SMa1531 at 844.859 kb on + strand at 844.859 kb on + strand at 844.859 kb on + strand at 844.859 kb on + strand at 844.859 kb on + strand at 844.859 kb on + strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.860 kb on - strand at 844.861 kb on + strand at 845.016 kb on + strand, within SMa1532 at 845.016 kb on + strand, within SMa1532 at 845.016 kb on + strand, within SMa1532 at 845.017 kb on - strand, within SMa1532 at 845.017 kb on - strand, within SMa1532 at 845.017 kb on - strand, within SMa1532 at 845.025 kb on + strand, within SMa1532 at 845.025 kb on + strand, within SMa1532 at 845.026 kb on - strand, within SMa1532 at 845.202 kb on + strand, within SMa1532
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 842,153 + SMa1525 0.69 +1.5 842,166 + SMa1525 0.70 -1.2 842,167 - SMa1525 0.70 +0.4 842,167 - SMa1525 0.70 -0.2 842,182 - SMa1525 0.71 -0.5 842,182 - SMa1525 0.71 +0.9 842,187 + SMa1525 0.72 +0.1 842,188 - SMa1525 0.72 -0.1 842,188 - SMa1525 0.72 +0.1 842,188 - SMa1525 0.72 +2.5 842,188 - SMa1525 0.72 +0.7 842,211 + SMa1525 0.74 +0.4 842,211 + SMa1525 0.74 -1.3 842,316 + SMa1525 0.82 -0.1 842,317 - SMa1525 0.82 +0.7 842,317 - SMa1525 0.82 -0.8 842,434 + -0.4 842,434 + +4.4 842,550 + -0.5 842,550 + +0.7 842,675 - SMa1526 0.25 +0.8 842,874 + SMa1526 0.64 +0.0 842,892 + SMa1526 0.68 -0.7 843,075 + +0.5 843,138 + -0.2 843,139 - +0.2 843,238 - SMa1529 0.13 -1.1 843,238 - SMa1529 0.13 -0.3 843,238 - SMa1529 0.13 +0.0 843,238 - SMa1529 0.13 -0.4 843,277 - SMa1529 0.17 -3.5 843,391 + SMa1529 0.26 -0.1 843,538 - SMa1529 0.38 +0.5 843,538 - SMa1529 0.38 -0.9 843,655 + SMa1529 0.48 +0.1 843,684 - SMa1529 0.50 +0.2 843,696 + SMa1529 0.51 -0.4 843,719 + SMa1529 0.53 +0.1 843,793 - SMa1529 0.59 +0.3 843,865 - SMa1529 0.65 -0.5 843,895 + SMa1529 0.68 -0.9 843,961 + SMa1529 0.73 -1.9 843,963 + SMa1529 0.73 -0.7 843,964 - SMa1529 0.73 +0.3 843,987 - SMa1529 0.75 -0.2 844,047 + SMa1529 0.80 +0.4 844,125 + SMa1529 0.87 -0.6 844,133 - SMa1529 0.87 -2.3 844,156 - SMa1529 0.89 -0.5 844,156 - SMa1529 0.89 -0.4 844,323 + +1.2 844,324 - -0.0 844,325 + -0.6 844,326 - -0.1 844,378 - SMa1531 0.13 -3.8 844,397 - SMa1531 0.17 +0.6 844,453 + SMa1531 0.27 +0.1 844,453 + SMa1531 0.27 -2.3 844,453 + SMa1531 0.27 -1.0 844,495 + SMa1531 0.35 +3.7 844,495 + SMa1531 0.35 -0.5 844,495 + SMa1531 0.35 -0.2 844,495 + SMa1531 0.35 -1.1 844,496 - SMa1531 0.35 -0.7 844,496 - SMa1531 0.35 -0.3 844,500 - SMa1531 0.36 -1.9 844,500 - SMa1531 0.36 -1.0 844,618 + SMa1531 0.57 -0.4 844,618 + SMa1531 0.57 +0.0 844,618 + SMa1531 0.57 +0.2 844,627 + SMa1531 0.59 -0.1 844,627 + SMa1531 0.59 +0.4 844,628 - SMa1531 0.59 -2.5 844,674 + SMa1531 0.67 +1.8 844,674 + SMa1531 0.67 +0.7 844,675 - SMa1531 0.67 +0.3 844,675 - SMa1531 0.67 +1.1 844,765 + SMa1531 0.84 +3.7 844,765 + SMa1531 0.84 -0.2 844,765 + SMa1531 0.84 -0.8 844,766 - SMa1531 0.84 +0.1 844,766 - SMa1531 0.84 +2.7 844,859 + -1.1 844,859 + -0.4 844,859 + +0.2 844,859 + -0.2 844,859 + -3.1 844,859 + +0.7 844,860 - -2.2 844,860 - +1.9 844,860 - +0.6 844,860 - +1.4 844,860 - +0.0 844,860 - +0.2 844,860 - -0.6 844,860 - +0.4 844,860 - +0.1 844,861 + +0.1 845,016 + SMa1532 0.33 +1.0 845,016 + SMa1532 0.33 +0.3 845,016 + SMa1532 0.33 +1.0 845,017 - SMa1532 0.33 -0.6 845,017 - SMa1532 0.33 +2.8 845,017 - SMa1532 0.33 +0.5 845,025 + SMa1532 0.35 -1.1 845,025 + SMa1532 0.35 +0.2 845,026 - SMa1532 0.35 +0.3 845,202 + SMa1532 0.70 -1.6
Or see this region's nucleotide sequence