Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1321 and SMa1322 are separated by 3 nucleotides SMa1322 and SMa1323 are separated by 96 nucleotides SMa1323 and SMa1325 are separated by 260 nucleotides SMa1325 and SMa1326 are separated by 73 nucleotides
SMa1321: SMa1321 - VirB1 type IV secretion protein, at 720,146 to 720,817
SMa1321
SMa1322: SMa1322 - hypothetical protein, at 720,821 to 721,339
SMa1322
SMa1323: SMa1323 - Transcription regulator, at 721,436 to 721,804
SMa1323
SMa1325: SMa1325 - hypothetical protein, at 722,065 to 722,316
SMa1325
SMa1326: SMa1326 - hypothetical protein, at 722,390 to 723,037
SMa1326
Position (kb)
721
722 Strain fitness (log2 ratio)
-2
-1
0
1 at 720.499 kb on - strand, within SMa1321 at 720.499 kb on - strand, within SMa1321 at 720.773 kb on + strand at 720.773 kb on + strand at 720.774 kb on - strand at 720.774 kb on - strand at 720.775 kb on + strand at 720.775 kb on + strand at 720.870 kb on + strand at 720.871 kb on - strand at 720.910 kb on - strand, within SMa1322 at 720.910 kb on - strand, within SMa1322 at 721.024 kb on + strand, within SMa1322 at 721.024 kb on + strand, within SMa1322 at 721.080 kb on + strand, within SMa1322 at 721.081 kb on - strand, within SMa1322 at 721.081 kb on - strand, within SMa1322 at 721.083 kb on + strand, within SMa1322 at 721.093 kb on - strand, within SMa1322 at 721.234 kb on + strand, within SMa1322 at 721.268 kb on - strand, within SMa1322 at 721.284 kb on + strand, within SMa1322 at 721.285 kb on - strand, within SMa1322 at 721.295 kb on + strand at 721.296 kb on - strand at 721.474 kb on - strand, within SMa1323 at 721.572 kb on - strand, within SMa1323 at 721.687 kb on + strand, within SMa1323 at 721.687 kb on + strand, within SMa1323 at 721.768 kb on - strand at 721.823 kb on - strand at 721.918 kb on + strand at 721.918 kb on + strand at 721.918 kb on + strand at 721.919 kb on - strand at 721.922 kb on - strand at 722.206 kb on + strand, within SMa1325 at 722.253 kb on - strand, within SMa1325 at 722.299 kb on + strand at 722.300 kb on - strand at 722.300 kb on - strand at 722.300 kb on - strand at 722.300 kb on - strand at 722.300 kb on - strand at 722.324 kb on + strand at 722.325 kb on - strand at 722.335 kb on + strand at 722.336 kb on - strand at 722.336 kb on - strand at 722.363 kb on + strand at 722.364 kb on - strand at 722.378 kb on + strand at 722.439 kb on + strand at 722.439 kb on + strand at 722.527 kb on + strand, within SMa1326 at 722.527 kb on + strand, within SMa1326 at 722.540 kb on + strand, within SMa1326 at 722.541 kb on - strand, within SMa1326
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 720,499 - SMa1321 0.53 +0.5 720,499 - SMa1321 0.53 -0.3 720,773 + +0.2 720,773 + -1.1 720,774 - -0.5 720,774 - -0.1 720,775 + -0.9 720,775 + -1.0 720,870 + -1.5 720,871 - -1.1 720,910 - SMa1322 0.17 -2.3 720,910 - SMa1322 0.17 -0.4 721,024 + SMa1322 0.39 -0.2 721,024 + SMa1322 0.39 -0.0 721,080 + SMa1322 0.50 -0.8 721,081 - SMa1322 0.50 +1.6 721,081 - SMa1322 0.50 +0.6 721,083 + SMa1322 0.50 +1.7 721,093 - SMa1322 0.52 +0.4 721,234 + SMa1322 0.80 -1.5 721,268 - SMa1322 0.86 +0.7 721,284 + SMa1322 0.89 -0.3 721,285 - SMa1322 0.89 -0.0 721,295 + -0.1 721,296 - -2.0 721,474 - SMa1323 0.10 -0.0 721,572 - SMa1323 0.37 -2.0 721,687 + SMa1323 0.68 -1.4 721,687 + SMa1323 0.68 -2.3 721,768 - -0.4 721,823 - -0.5 721,918 + -2.1 721,918 + -0.3 721,918 + -2.0 721,919 - +0.4 721,922 - -0.7 722,206 + SMa1325 0.56 +0.1 722,253 - SMa1325 0.75 -0.2 722,299 + -1.2 722,300 - -1.6 722,300 - -2.1 722,300 - -2.1 722,300 - +0.6 722,300 - +0.8 722,324 + -2.1 722,325 - -0.1 722,335 + +1.1 722,336 - -0.4 722,336 - -1.5 722,363 + -1.2 722,364 - -0.4 722,378 + -0.0 722,439 + +0.3 722,439 + -0.4 722,527 + SMa1326 0.21 +0.6 722,527 + SMa1326 0.21 +0.0 722,540 + SMa1326 0.23 +0.4 722,541 - SMa1326 0.23 +0.6
Or see this region's nucleotide sequence