Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0637 and SMa0638 overlap by 4 nucleotides SMa0638 and SMa0639 are separated by 404 nucleotides SMa0639 and SMa0640 are separated by 90 nucleotides
SMa0637: SMa0637 - hypothetical protein, at 342,592 to 342,981
SMa0637
SMa0638: SMa0638 - hypothetical protein, at 342,978 to 343,235
SMa0638
SMa0639: SMa0639 - hypothetical protein, at 343,640 to 344,044
SMa0639
SMa0640: SMa0640 - hypothetical protein, at 344,135 to 344,554
SMa0640
Position (kb)
343
344
345 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 342.726 kb on - strand, within SMa0637 at 342.727 kb on + strand, within SMa0637 at 342.727 kb on + strand, within SMa0637 at 342.727 kb on + strand, within SMa0637 at 342.727 kb on + strand, within SMa0637 at 342.727 kb on + strand, within SMa0637 at 342.727 kb on + strand, within SMa0637 at 342.728 kb on - strand, within SMa0637 at 342.728 kb on - strand, within SMa0637 at 342.728 kb on - strand, within SMa0637 at 342.873 kb on + strand, within SMa0637 at 342.874 kb on - strand, within SMa0637 at 342.932 kb on + strand, within SMa0637 at 343.036 kb on - strand, within SMa0638 at 343.180 kb on - strand, within SMa0638 at 343.363 kb on - strand at 343.446 kb on + strand at 343.446 kb on + strand at 343.447 kb on - strand at 343.447 kb on - strand at 343.519 kb on + strand at 343.519 kb on + strand at 343.571 kb on + strand at 343.579 kb on - strand at 343.583 kb on - strand at 343.596 kb on - strand at 343.683 kb on + strand, within SMa0639 at 343.683 kb on + strand, within SMa0639 at 343.684 kb on - strand, within SMa0639 at 343.687 kb on + strand, within SMa0639 at 343.687 kb on + strand, within SMa0639 at 343.688 kb on - strand, within SMa0639 at 343.688 kb on - strand, within SMa0639 at 343.688 kb on - strand, within SMa0639 at 343.688 kb on - strand at 343.688 kb on - strand, within SMa0639 at 343.716 kb on + strand, within SMa0639 at 343.756 kb on + strand, within SMa0639 at 343.757 kb on - strand, within SMa0639 at 343.757 kb on - strand, within SMa0639 at 343.757 kb on - strand, within SMa0639 at 343.811 kb on + strand, within SMa0639 at 343.852 kb on - strand, within SMa0639 at 343.965 kb on - strand, within SMa0639 at 343.965 kb on - strand, within SMa0639 at 344.036 kb on + strand at 344.036 kb on + strand at 344.036 kb on + strand at 344.037 kb on - strand at 344.037 kb on - strand at 344.180 kb on - strand, within SMa0640 at 344.268 kb on + strand, within SMa0640 at 344.269 kb on - strand, within SMa0640 at 344.277 kb on + strand, within SMa0640 at 344.277 kb on + strand, within SMa0640 at 344.292 kb on - strand, within SMa0640 at 344.352 kb on + strand, within SMa0640 at 344.503 kb on - strand, within SMa0640 at 344.673 kb on + strand at 344.704 kb on + strand at 344.704 kb on + strand at 344.705 kb on - strand at 344.705 kb on - strand at 344.705 kb on - strand at 344.705 kb on - strand at 344.840 kb on + strand at 344.853 kb on - strand at 344.853 kb on - strand at 344.920 kb on + strand at 344.921 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 342,726 - SMa0637 0.34 -0.3 342,727 + SMa0637 0.35 -0.5 342,727 + SMa0637 0.35 +0.1 342,727 + SMa0637 0.35 -0.3 342,727 + SMa0637 0.35 +0.1 342,727 + SMa0637 0.35 -1.5 342,727 + SMa0637 0.35 +0.5 342,728 - SMa0637 0.35 -0.6 342,728 - SMa0637 0.35 -0.2 342,728 - SMa0637 0.35 +0.7 342,873 + SMa0637 0.72 +0.1 342,874 - SMa0637 0.72 +2.2 342,932 + SMa0637 0.87 -0.2 343,036 - SMa0638 0.22 -0.3 343,180 - SMa0638 0.78 -0.8 343,363 - +0.9 343,446 + +1.4 343,446 + -0.5 343,447 - -0.4 343,447 - -2.5 343,519 + -0.4 343,519 + +0.5 343,571 + +3.6 343,579 - +0.2 343,583 - +0.7 343,596 - +0.8 343,683 + SMa0639 0.11 -0.3 343,683 + SMa0639 0.11 -0.1 343,684 - SMa0639 0.11 +0.1 343,687 + SMa0639 0.12 +0.5 343,687 + SMa0639 0.12 -1.2 343,688 - SMa0639 0.12 +0.4 343,688 - SMa0639 0.12 -0.3 343,688 - SMa0639 0.12 -0.0 343,688 - +1.7 343,688 - SMa0639 0.12 +0.7 343,716 + SMa0639 0.19 +1.6 343,756 + SMa0639 0.29 +0.2 343,757 - SMa0639 0.29 -0.3 343,757 - SMa0639 0.29 +1.7 343,757 - SMa0639 0.29 +0.4 343,811 + SMa0639 0.42 +0.5 343,852 - SMa0639 0.52 +2.0 343,965 - SMa0639 0.80 +0.1 343,965 - SMa0639 0.80 +0.4 344,036 + -0.2 344,036 + -1.6 344,036 + -0.2 344,037 - +0.2 344,037 - -0.6 344,180 - SMa0640 0.11 -0.1 344,268 + SMa0640 0.32 +0.1 344,269 - SMa0640 0.32 -0.7 344,277 + SMa0640 0.34 +0.7 344,277 + SMa0640 0.34 +0.5 344,292 - SMa0640 0.37 -0.5 344,352 + SMa0640 0.52 +1.0 344,503 - SMa0640 0.88 +0.4 344,673 + +0.6 344,704 + -0.9 344,704 + -1.7 344,705 - +0.5 344,705 - +0.1 344,705 - -0.9 344,705 - +0.6 344,840 + -0.3 344,853 - +0.2 344,853 - -2.3 344,920 + -0.1 344,921 - +0.2
Or see this region's nucleotide sequence